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Effect of photocatalytic pretreatment of potato starch for bioethanol production using Saccharomyces cerevisiae during simultaneous saccharification-fermentation (SSF)
Efecto del pretratamiento fotocatalítico de almidón de papa para la producción de bioetanol utilizando Saccharomyces cerevisiae durante fermentación-sacarificación simultánea
DOI:
https://doi.org/10.15446/dyna.v86n208.72695Palabras clave:
bioethanol, photocatalytic pretreatment, potato starch. (en)bioetanol, pretratamiento fotocatalítico, almidón de papa. (es)
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In this study, the effect of the photocatalytic (PC) pre-treatment with TiO2 during gelatinization (GE) stage in a simultaneous saccharification-fermentation process (SSF) of potato starch for bioethanol production was evaluated. Maximum amount of reducing sugars was 119.3, 114.6 and 104.8 g l-1 for PC→GE, GE→PC and reference (without PC), respectively while bioethanol concentration it increased gradually up to a maximum amount of 128.21, 106.74 and 85.91 g l-1 after 30 h, for PC→GE, GE→PC and reference (without PC), respectively. These results were consistent with the reducing sugars concentration, because bioethanol concentration increased slightly during 18–30 h of fermentation. Although enzymatic activity (ʋmax) for reactions was similar, in reference (without PC pre-treatment) it not promoted quick substrate conversion into ethanol, despite showing the higher affinity enzyme-substrate (Km). Considering traditional potato starch hydrolysis, PC pre-treatment shortened the reaction time of the biological reactions. Thus, the PC pre-treatment of potato starch for bioethanol production could be an environmentally feasible process without acid and alkali addition.
En este estudio, se evaluó el efecto del pretratamiento fotocatalítico (PC) con TiO2 durante la etapa de gelatinización (GE) en un proceso simultáneo de sacarificación-fermentación (SSF) del almidón de papa para la producción de bioetanol. La cantidad máxima de azúcares reductores fue 119.3, 114.6 y 104.8 g l-1 para PC → GE, GE → PC y referencia (sin PC), respectivamente mientras que la concentración de bioetanol aumentó gradualmente hasta una cantidad máxima de 128.21, 106.74 y 85.91 g l-1 después de 30 h, para PC → GE, GE → PC y referencia (sin PC). Estos resultados fueron consistentes con la concentración de azúcares reductores, debido a que la concentración de bioetanol aumentó ligeramente durante 18-30 h de fermentación. Aunque la actividad enzimática (ʋmax) para las reacciones fue similar, sin pretratamiento fotocatalítico (referencia) no se promovió la rápida conversión de sustrato en etanol, a pesar de mostrar mayor afinidad (Km) con el sustrato enzimático. Considerando la hidrólisis tradicional de almidón de papa, el pretratamiento fotocatalítico (PC) disminuyó el tiempo de las reacciones biológicas. De esta forma, el pretratamiento fotocatalítico de almidón de papa para la producción de bioetanol podría ser un proceso factible ambientalmente sin adición de ácido y álcali.
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