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Significant enzymatic activities in the residues hydrolysis of the sugar cane harvest
Actividades enzimáticas significativas en la hidrólisis de residuos de la cosecha de caña de azúcar
DOI:
https://doi.org/10.15446/dyna.v86n210.75286Palabras clave:
top cane, leaves, sugar cane, hemicellulose, cellulases, enzymatic hydrolysis (en)tallo, hojas, caña de azúcar, hemicelulasas., celulasas, hidrólisis enzimática (es)
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In the production of ethanol from agroindustrial crop residues, one of the critical stages in the process is the conversion of lignocellulosic material to simple sugars, which can be done chemically or enzymatically. In this research, the enzymatic activities of commercial enzymes were evaluated for their influence on the degradation of lignocellulosic materials from sugar cane harvest residues (leaves and top cane). Eight substrates were pretreated with different delignification methods. Likewise, five enzymatic preparations were configured. An analysis of the enzyme-substrate interactions was conducted through fuzzy system analysis. The results showed regions of maximum enzymatic activity for residues of the sugarcane harvest, between 20-30 Filter Paper Units (FPU) /mL values lower than 500 pNPG (p-Nitrofenol-α-D-Glucopyranoside) U / mL of activity beta-glucosidase and hemicellulase activity between 50 and 70 IU / mL, confirming that the use of large amounts of cellulolytic enzymes is not necessary.
En la producción de etanol a partir de residuos agroindustriales, una de las etapas críticas en el proceso es la conversión del material lignocelulósico a azúcares simples, que puede realizarse química o enzimáticamente. En esta investigación, se evaluó la influencia de las actividades enzimáticas de las enzimas comerciales para degradar materiales de residuos de cosecha de la caña de azúcar (hojas y cogollos). Ocho sustratos fueron pretratados con diferentes métodos de deslignificación, con cinco preparaciones enzimáticas. Se utilizó un análisis de las interacciones enzima-sustrato, a través del análisis del sistema difuso. Los resultados mostraron regiones de actividad enzimática entre 20-30 FPU / mL y valores inferiores a 500 pNPG U / mL de actividad beta-glucosidasa y para actividad hemicelulasa entre 50 y 70 IU / mL, confirmando que el uso de grandes cantidades de enzimas celulolíticas no es necesario.
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