Publicado

2020-07-01

Cutinases obtained from filamentous fungi: comparison of screening methods

Cutinasas obtenidas de hongos filamentos: comparación de métodos de screening

Palabras clave:

filamentous fungi, cutinases, cutinase activity, agroindustrial waste, flaxseed oil (en)
hongos filamentosos, cutinasas, actividad cutinasa, residuos agroindustriales, aceite de linasa (es)

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Cutinases are secreted by filamentous fungi that and hydrolyze polymers. However, few selection methods for cutinases are available. Here, we studied three screening methods using 33 strains of filamentous fungi isolated from banana rachis with high potential to produce cutinases. In the first method, strains were grown in Czapec-Dox mineral medium containing flaxseed oil. We note that six strains of the genera Fusarium, Penicillium, and Mucor had cutinase activity. The second method evaluated strains with triacetin in rhodamine B, which indicated what strains had esterase property. Finally, strains were subjected to fermentation with flaxseed oil; lipolytic and cutinolytic activity were determined. The species identified as the best producers of cutinases were Fusarium fujikuroi and Penicillium chrysogenum, and we obtained two extracellular cutinases with activities of 33.5 U/mL and 39.4 U/mL respectively. Cutinase was confirmed via degradation of tomato cutin through FTIR.

Las cutinasas son secretadas por hongos filamentosos que hidrolizan polímeros. Sin embargo, pocos métodos de selección están disponibles. En este trabajo, tres métodos fueron estudiados, usando 33 cepas de hongos filamentosos aislados de banana rachis con alto potencial para producir cutinasas. En el primer método, las cepas fueron crecidas en medio mineral Czapec-Dox que contenía aceite de linasa, encontrándose que seis cepas de los géneros Fusarium, Penicillium and Mucor podrían tener actividad cutinasa. El segundo método consistió en evaluar las cepas con triacetina en rodamina, indicando que cepas tenían propiedades esterasas. Finalmente, las cepas fueron sometidas a fermentación con aceite de linasa; actividad lipolítica y cutinolítica fue determinada. Las especies identificadas como las mejores productoras de cutinasas fueron Fusarium fujikuroi y Penicillium chrysogenum, obteniéndose 2 cutinasas extracelulares con actividades de 33.5 U/mL y 39.4 U/mL respectivamente. Las cutinasas fueron confirmadas por degradación de cutina de tomate a través de FTIR.

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