Publicado

2020-07-01

Cutinases obtained from filamentous fungi: comparison of screening methods

Cutinasas obtenidas de hongos filamentos: comparación de métodos de screening

DOI:

https://doi.org/10.15446/dyna.v87n214.83737

Palabras clave:

filamentous fungi, cutinases, cutinase activity, agroindustrial waste, flaxseed oil (en)
hongos filamentosos, cutinasas, actividad cutinasa, residuos agroindustriales, aceite de linasa (es)

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Cutinases are secreted by filamentous fungi that and hydrolyze polymers. However, few selection methods for cutinases are available. Here, we studied three screening methods using 33 strains of filamentous fungi isolated from banana rachis with high potential to produce cutinases. In the first method, strains were grown in Czapec-Dox mineral medium containing flaxseed oil. We note that six strains of the genera Fusarium, Penicillium, and Mucor had cutinase activity. The second method evaluated strains with triacetin in rhodamine B, which indicated what strains had esterase property. Finally, strains were subjected to fermentation with flaxseed oil; lipolytic and cutinolytic activity were determined. The species identified as the best producers of cutinases were Fusarium fujikuroi and Penicillium chrysogenum, and we obtained two extracellular cutinases with activities of 33.5 U/mL and 39.4 U/mL respectively. Cutinase was confirmed via degradation of tomato cutin through FTIR.

Las cutinasas son secretadas por hongos filamentosos que hidrolizan polímeros. Sin embargo, pocos métodos de selección están disponibles. En este trabajo, tres métodos fueron estudiados, usando 33 cepas de hongos filamentosos aislados de banana rachis con alto potencial para producir cutinasas. En el primer método, las cepas fueron crecidas en medio mineral Czapec-Dox que contenía aceite de linasa, encontrándose que seis cepas de los géneros Fusarium, Penicillium and Mucor podrían tener actividad cutinasa. El segundo método consistió en evaluar las cepas con triacetina en rodamina, indicando que cepas tenían propiedades esterasas. Finalmente, las cepas fueron sometidas a fermentación con aceite de linasa; actividad lipolítica y cutinolítica fue determinada. Las especies identificadas como las mejores productoras de cutinasas fueron Fusarium fujikuroi y Penicillium chrysogenum, obteniéndose 2 cutinasas extracelulares con actividades de 33.5 U/mL y 39.4 U/mL respectivamente. Las cutinasas fueron confirmadas por degradación de cutina de tomate a través de FTIR.

Referencias

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Cómo citar

IEEE

[1]
H. A. Rueda Rueda, carlos alberto jimenez-junca, y R. E. Prieto Correa, «Cutinases obtained from filamentous fungi: comparison of screening methods», DYNA, vol. 87, n.º 214, pp. 183–190, jul. 2020.

ACM

[1]
Rueda Rueda, H.A., jimenez-junca, carlos alberto y Prieto Correa, R.E. 2020. Cutinases obtained from filamentous fungi: comparison of screening methods. DYNA. 87, 214 (jul. 2020), 183–190. DOI:https://doi.org/10.15446/dyna.v87n214.83737.

ACS

(1)
Rueda Rueda, H. A.; jimenez-junca, carlos alberto; Prieto Correa, R. E. Cutinases obtained from filamentous fungi: comparison of screening methods. DYNA 2020, 87, 183-190.

APA

Rueda Rueda, H. A., jimenez-junca, carlos alberto & Prieto Correa, R. E. (2020). Cutinases obtained from filamentous fungi: comparison of screening methods. DYNA, 87(214), 183–190. https://doi.org/10.15446/dyna.v87n214.83737

ABNT

RUEDA RUEDA, H. A.; JIMENEZ-JUNCA, carlos alberto; PRIETO CORREA, R. E. Cutinases obtained from filamentous fungi: comparison of screening methods. DYNA, [S. l.], v. 87, n. 214, p. 183–190, 2020. DOI: 10.15446/dyna.v87n214.83737. Disponível em: https://revistas.unal.edu.co/index.php/dyna/article/view/83737. Acesso em: 16 mar. 2026.

Chicago

Rueda Rueda, Hugo Alfonso, carlos alberto jimenez-junca, y Rosa Erlide Prieto Correa. 2020. «Cutinases obtained from filamentous fungi: comparison of screening methods». DYNA 87 (214):183-90. https://doi.org/10.15446/dyna.v87n214.83737.

Harvard

Rueda Rueda, H. A., jimenez-junca, carlos alberto y Prieto Correa, R. E. (2020) «Cutinases obtained from filamentous fungi: comparison of screening methods», DYNA, 87(214), pp. 183–190. doi: 10.15446/dyna.v87n214.83737.

MLA

Rueda Rueda, H. A., carlos alberto jimenez-junca, y R. E. Prieto Correa. «Cutinases obtained from filamentous fungi: comparison of screening methods». DYNA, vol. 87, n.º 214, julio de 2020, pp. 183-90, doi:10.15446/dyna.v87n214.83737.

Turabian

Rueda Rueda, Hugo Alfonso, carlos alberto jimenez-junca, y Rosa Erlide Prieto Correa. «Cutinases obtained from filamentous fungi: comparison of screening methods». DYNA 87, no. 214 (julio 1, 2020): 183–190. Accedido marzo 16, 2026. https://revistas.unal.edu.co/index.php/dyna/article/view/83737.

Vancouver

1.
Rueda Rueda HA, jimenez-junca carlos alberto, Prieto Correa RE. Cutinases obtained from filamentous fungi: comparison of screening methods. DYNA [Internet]. 1 de julio de 2020 [citado 16 de marzo de 2026];87(214):183-90. Disponible en: https://revistas.unal.edu.co/index.php/dyna/article/view/83737

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CrossRef Cited-by

CrossRef citations8

1. Aruna Singh Parmar, Deepak K. Rahi. (2023). Cutinase production from Fusarium verticillioides using response surface methodology and its application as potential insecticide degrader. Environmental Science and Pollution Research, 30(36), p.86484. https://doi.org/10.1007/s11356-023-28635-1.

2. Fengjuan Lang, Fan Fei, Chaomin Sun, Shimei Wu, Ning-Yi Zhou. (2025). Highly efficient degradation of polybutylene succinate (PBS) and polycaprolactone (PCL) by a recombinant marine fungal cutinase. Applied and Environmental Microbiology, 91(9) https://doi.org/10.1128/aem.00833-25.

3. Arman Mussakhmetov, Dmitriy Silayev. (2025). Esterases: Mechanisms of Action, Biological Functions, and Application Prospects. Applied Microbiology, 5(4), p.139. https://doi.org/10.3390/applmicrobiol5040139.

4. Xiuhong Liang, Huibin Zou. (2022). Biotechnological Application of Cutinase: A Powerful Tool in Synthetic Biology. SynBio, 1(1), p.54. https://doi.org/10.3390/synbio1010004.

5. Pinku Chandra Nath, Jibanjyoti Panda, Laikhuram Sarda Devi, Yugal Kishore Mohanta, Mohammad Zaki Shamim, Prakash Kumar Nayak. (2025). Engineering Solutions for Sustainable Food and Dairy Production. Food Engineering Series. , p.345. https://doi.org/10.1007/978-3-031-75834-8_13.

6. María Camila Sinisterra-Sierra, Amador Campos-Valdez, Alejandro Pereira-Santana, Jesús Alejandro Zamora-Briseño, Sandra L. Ramírez-Pérez, Jorge L. González-Escobar, Manuel R. Kirchmayr, Iliana Barrera-Martínez, Marcela Robles-Machuca, Leticia Casas-Godoy. (2025). Microbial diversity and enzymatic potential for plastic degradation in contaminated dumpsites in Mazamitla, Jalisco. Environmental Research, 283, p.122170. https://doi.org/10.1016/j.envres.2025.122170.

7. Francesco Degli-Innocenti, Tony Breton, Selene Chinaglia, Ermes Esposito, Marco Pecchiari, Andrea Pennacchio, Alessandro Pischedda, Maurizio Tosin. (2023). Microorganisms that produce enzymes active on biodegradable polyesters are ubiquitous. Biodegradation, 34(6), p.489. https://doi.org/10.1007/s10532-023-10031-8.

8. Asiya Azarudeen, Sam Peniel Richard, Tamilarasi Sambu Periyasamy, Nishu Sekar, Hariprasath Lakshmanan. (2025). In silico engineering of Aspergillus tubingensis cutinase to enhance PET biodegradation potential. Environmental Science and Pollution Research, 32(46), p.26088. https://doi.org/10.1007/s11356-025-37179-5.

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