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Chlamydospores production enhancement of Duddingtonia flagrans in a solid-state fermentation system
Mejora de la producción de clamidosporas de Duddingtonia flagrans en un sistema de fermentación en estado sólido
DOI:
https://doi.org/10.15446/dyna.v88n216.85978Palabras clave:
Placket Burman design; central compose design; nematode trapping fungi; ammonium sulphate and sodium acetate (en)diseño Plackett Burman; diseño central compuesto; hongos nematofagos; sulfato de amonio y acetato de sodio (es)
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Resistance structures such as chlamydospores produced by the fungus Duddingtonia flagrans allow the reduction of infectious larvae from gastrointestinal nematodes. The objective of this research was to study the effect of carbon and nitrogen sources on the production of chlamydospores of the fungus in a solid state fermentation system (SSF). Twelve substances were studied using a statistical strategy, evaluating their effect on the production of Chlamydospores Finally, using an optimization strategy, the modifications of the substances that favor the production of chlamydospores were defined, and the effect of these on the predatory capacity of the fungus was evaluated. Optimal conditions were the variability of 0.25% w / w ammonium sulfate and 0.56% w / w sodium acetate in broken rice. The maximum concentration reached under this condition was 2.27x107 chlamydospores g of dry substrate-1, with a productivity of 1.62x106 chlamydospores g of dry substrate-1 day-1.
Estructuras de resistencia como las clamidosporas producidas por el hongo Duddingtonia flagrans permiten la reducción de larvas infecciosas de nematodos gastrointestinales. El objetivo de esta investigación fue estudiar el efecto de fuentes de carbono y nitrógeno sobre la producción de clamidosporas del hongo en un sistema de fermentación en estado sólido (SSF), doce sustancias fueron estudiadas mediante una estrategia estadística, evaluando su efecto sobre la producción de clamidosporas. Finalmente, usando una estrategia de optimización se definió las concentraciones de las sustancias que favorecían la producción de clamidosporas, y se evaluó el efecto de estas sobre la capacidad predatoria del hongo. Las condiciones óptimas fueron la adición de 0.25% p/p de sulfato de amonio y 0.56% p/p de acetato de sodio al arroz partido. La concentración máxima alcanzada bajo esta condición fue de 2.27x107 clamidosporas g de sustrato seco-1, con una productividad de 1.62x106 clamidosporas g de sustrato seco-1 día-1.
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3. M. Junco, L.E. Iglesias, S. Zegbi, M.F. Sagués, I. Guerrero, G. Bernat, M.E. Fuentes, E. Riva, A.S. Fernández, C.A. Saumell. (2024). Enhancing chlamydospore production in Duddingtonia flagrans on solid substrate: The impact of mannitol and varied cultivation conditions. Experimental Parasitology, 260, p.108725. https://doi.org/10.1016/j.exppara.2024.108725.
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