Publicado

2021-09-10

In vitro evaluation the toxicity in mixture of glyphosate and methyl metsulfuron against strains of Trichoderma spp

Evaluación in vitro de la toxicidad de los herbicidas glifosato y metsulfurón metil frente a cepas de Trichoderma spp

DOI:

https://doi.org/10.15446/dyna.v88n218.92420

Palabras clave:

Trichoderma, herbicides, antagonims; toxicity, gliphosate, metsulfuron-methyl (en)
Trichoderma, antagonismo, herbicidas, glifosato, metsolfuron metil (es)

Autores/as

Trichoderma is an antagonist fungus used in agriculture and forestry for the diseases control. The use of the herbicides metsulfuton methyl and glyphosate is a common practice for weed control that limits Trichoderma use. This study has evaluated the toxic effects ofthe herbicides metsulfuron methyl and glyphosate on four strains of fungi of the genus Trichoderma (GRB-HA1, GRB-HA2, GRB-HA8 and GRB-HA9). Poisoned media were prepared with high doses of 25 mL·L-1 of glyphosate + 0.2 g L-1 of metsulfuron methyl (D500) and low doses of 12.5 mL·L-1 of glyphosate + 0.2g L-1 of methyl metsulfuron (D250) to determine the effects on viability. The effects of the exposure time (30-90 min) on the germination of the conidia were determined using suspensions prepared with the mixtures D500 and D250. The results showed the differential sensitivity of the Trichoderma strains that affected the viability of the conidia and mycelial growth

Trichoderma es un hongo antagonista utilizado en agricultura y silvicultura para el control de enfermedades. El uso de los herbicidas metsulfuton metil y glifosato es una práctica común que limita las aplicaciones de trichoderma. Este estudio evaluó los efectos tóxicos de los herbicidas metsulfuron metil y glifosato sobre cuatro cepas de hongos del género Trichoderma (GRB-HA1, GRB-HA2, GRB-HA8 y GRB-HA9). para determinar los efectos sobre la viabilidad se usaron medios envenenados con dosis altas (25 mL·L-1 de glifosato + 0.2 g L-1 de metsulfuron metil; D500) y dosis bajas (12.5 mL·L-1 de glifosato + 0.2 g L-1 de metsulfuron metsulfuron; D250). Los efectos del tiempo de exposición (30-90 min) sobre la germinación de los conidios se determinaron utilizando suspensiones preparadas con las mezclas D500 y D250. Los resultados evidenciaron la sensibilidad diferencial de las cepas de trichoderma que afectaron la viabilidad de los conidios y el crecimiento micelial.

Referencias

Perfetti, J. J., Balcázar, Á., Hernández, A., and Leibovich, J. Políticas para el desarrollo de la agricultura en Colombia. Sociedad de Agricultores de Colombia, Fedesarrollo. 2013.

Barrios, A., López A., M. Nieto V. Predicción de volúmenes comerciales de Eucalyptus grandis a través de modelos de volumen total y de razón. Colombia Forestal, 17 (2), pp 137-149. 2014. http://dx.doi.org/10.14483/udistrital.jour.colomb.for.2014.2.a01.

Valdés E. L. Caracteres principales, ventajas y beneficios agrícolas que aporta el uso de Trichoderma como control biológico. Agroecosistemas. 2 (1), pp 254-264. 2014.Available at: https://aes.ucf.edu.cu/index.php/aes/article/view/40.

Fuentes, C., and Ferrucho, J. Actividad del azimsulfuron + metsulfuron-metil en Cyperus rotundus L. bajo condiciones controladas. Agronomía Colombiana. 19(1-2 pp37–42, 2011. Available at: http://revistas.unal.edu.co/index.php/agrocol/article/view/21878.

Santana-Díaz, T., and Castellanos-González, L. Efecto bioestimulante de Trichoderma harzianum Rifai en posturas de Leucaena, Cedro y Samán. Colombia Forestal. 21 (1), pp81-90. 2017. http://dx.doi.org/10.14483/2256201X.11744.

Duncan, DR and JD Paxton. Trifluralin enhancement of Phytophthora root rot of soybean. Plant Dis. 65(5), pp435- 436. 1981. http://dx.doi.org/10.1094/PD-65-435.

Javaid, A., Shafique, G., Ali, S., and Shoaib, A. Effect of Culture Medium on Herbicidal Potential of Metabolites of Trichoderma Species against Parthenium hysterophorus. International Journal of Agricuture & Biology. 15(1), pp 119–14. 2013.Available at: http://www.fspublishers.org/published_papers/33360.pdf.

Benamú, M. A., Schneider, M. I., and Sánchez, N. E. Effects of the herbicide glyphosate on biological attributes of Alpaida veniliae (Araneae, Araneidae), in laboratory. Chemosphere. pp 871–876. 78(7), 2010. http://doi.org/10.1016/j.chemosphere.2009.11.02.

Wilcox, WF. Influence of dinitroanaline herbicides on growth, sporulation, and infectivety of four Phytophthora spp. pathogenic to deciduous fruit trees. Phytopathology. 86(9), pp 906-913. 1996. http://dx.doi.org/2F10.1094%2FPhyto-86-906.

Bórtoli, Paula V, Verdenelli, Romina A, Conforto, Cinthia, Vargas Gil, Silvina, and Meriles, José M. Efectos del herbicida glifosato sobre la estructura y el funcionamiento de comunidades microbianas de dos suelos de plantaciones de olivo. Ecología austral. 2(1), pp33-42. 2012.Available at: https://bibliotecadigital.exactas.uba.ar/download/ecologiaaustral/ecologiaaustral_v022_n01_p033.pdf.

Vázquez, M. B., and Bianchinotti, M. V. Fungi degrading metsulfuron methyl in agricultural soils of Argentina. Phyton, 82(1), pp 113–118. 2013. http://doi.org/10.32604/phyton.2013.82.113.

Ranganathaswamy, M., Patibanda, A. K., and Rao, G. N. Evaluation of toxicity of agrochemicals on Trichoderma isolates in vitro. Journal of Biological Control, 26 (4), pp391–395. 2012.Available at: http://journalofbiologicalcontrol.com/index.php/jbc/article/view/46795.

Chan, W., Heredia, G., Rodriguez, R., and Arias, R. Toxicidad in vitro de los herbicidas atrazina y paraquat sobre el crecimiento vegetativo y la esporulación de hongos saprobios del suelo. Revista Internacional de Contaminación Ambiental. 30(4), pp 393–406. 2014.Available at: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0188-49992014000400007&lng=es&nrm=iso.

Pabón-Baquero, D., Velázquez-del Valle, M. G., Evangelista-Lozano, S., León-Rodriguez, R., and Hernández-Lauzardo, A. N. Chitosan effects on phytopathogenic fungi and seed germination of Jatropha curcas L. Revista Chapingo Serie Ciencias Forestales Y Del Ambiente. 21(3), pp241–253. 2015. http://doi.org/10.5154/r.rchscfa.2014.10.051.

Panebianco, A., Castello, I., Cirvilleri, G., Perrone, G., Epifani, F., Ferrara, M., and Vitale, A. Detection of Botrytis cinerea field isolates with multiple fungicide resistance from table grape in Sicily. Crop Protection. 77 (1) pp65–73. 2015. http://doi.org/10.1016/j.cropro.2015.07.010.

Santoro, P. H., Cavaguchi, S. A., Moretto, T.A., Zorzetti, J., and Neves, Oliveira-Janeiro, P.M . In vitro sensitivity of antagonistic Trichoderma atroviride to herbicides. Brazilian Archives of Biology and Technology, 57(2). pp238-243. 2014. https://dx.doi.org/10.1590/S1516-89132014000200012.

Zain, N. M. M., Mohamad, R. B., Sijam, K., Morshed, M. M., and Awang, Y. Effect of selected herbicides in vitro and in soil on growth and development of soil fungi from oil palm plantation. African Journal of Microbiology Research. 15(5), pp820–826. 2013. http://doi.org/10.5897/AJMR12.12772013.

Melo R., A., Ariza, P., Lissbrant, S., and Tofiño, A. Evaluation of agrochemicals and bioinputs for sustainable bean management on the Caribbean coast of Colombia. Agronomía Colombiana. 33(2), pp203–211. 2015.http://doi.org/10.15446/agron.colomb.v33n2.49858.

Meriles, J. M., Vargas Gil, S., Haro, R. J., March, G. J., and Guzman, C. A. Glyphosate and previous crop residue effect on deleterious and beneficial soil-borne fungi from a peanut-corn-soybean rotation. Journal of Phytopathology. 154(5), pp309–316. 2006. http://doi.org/10.1111/j.1439-0434.2006.01098.x.

Sanogo S., Yang, X.B., and Scherm. H, Effects of herbicide on Fusarium solani f. sp. glycines and development of sudden death syndrome in glyphosate-tolerant soybean. Phytopathology, 90(1), pp.57-66, 2000. http://doi.org/10.1094/phyto.2000.90.1.57.

Druille, M., Cabello, M., Omacini, M. N., and Golluscio, R. A. Glyphosate reduces spore viability and root colonization of arbuscular mycorrhizal fungi. Applied Soil Ecology. 64(1), pp 99–103. 2013. http://doi.org/10.1016/j.apsoil.2012.10.007.

Cómo citar

IEEE

[1]
J. P. Ramirez Olier, H. D. . Gómez-Berrio, J. A. . Trujillo-Salazar, D. L. . Delgado-Gómez, C. S. . Rivera-Zuleta, y L. . Rocío Botero, «In vitro evaluation the toxicity in mixture of glyphosate and methyl metsulfuron against strains of Trichoderma spp», DYNA, vol. 88, n.º 218, pp. 224–229, jul. 2021.

ACM

[1]
Ramirez Olier, J.P., Gómez-Berrio, H.D. , Trujillo-Salazar, J.A. , Delgado-Gómez, D.L. , Rivera-Zuleta, C.S. y Rocío Botero, L. 2021. In vitro evaluation the toxicity in mixture of glyphosate and methyl metsulfuron against strains of Trichoderma spp. DYNA. 88, 218 (jul. 2021), 224–229. DOI:https://doi.org/10.15446/dyna.v88n218.92420.

ACS

(1)
Ramirez Olier, J. P.; Gómez-Berrio, H. D. .; Trujillo-Salazar, J. A. .; Delgado-Gómez, D. L. .; Rivera-Zuleta, C. S. .; Rocío Botero, L. . In vitro evaluation the toxicity in mixture of glyphosate and methyl metsulfuron against strains of Trichoderma spp. DYNA 2021, 88, 224-229.

APA

Ramirez Olier, J. P., Gómez-Berrio, H. D. ., Trujillo-Salazar, J. A. ., Delgado-Gómez, D. L. ., Rivera-Zuleta, C. S. . & Rocío Botero, L. . (2021). In vitro evaluation the toxicity in mixture of glyphosate and methyl metsulfuron against strains of Trichoderma spp. DYNA, 88(218), 224–229. https://doi.org/10.15446/dyna.v88n218.92420

ABNT

RAMIREZ OLIER, J. P.; GÓMEZ-BERRIO, H. D. .; TRUJILLO-SALAZAR, J. A. .; DELGADO-GÓMEZ, D. L. .; RIVERA-ZULETA, C. S. .; ROCÍO BOTERO, L. . In vitro evaluation the toxicity in mixture of glyphosate and methyl metsulfuron against strains of Trichoderma spp. DYNA, [S. l.], v. 88, n. 218, p. 224–229, 2021. DOI: 10.15446/dyna.v88n218.92420. Disponível em: https://revistas.unal.edu.co/index.php/dyna/article/view/92420. Acesso em: 7 mar. 2026.

Chicago

Ramirez Olier, Johana Patricia, Hernán David Gómez-Berrio, Juan Alberto Trujillo-Salazar, Dora Luz Delgado-Gómez, Claudia Sirley Rivera-Zuleta, y Liliana Rocío Botero. 2021. «In vitro evaluation the toxicity in mixture of glyphosate and methyl metsulfuron against strains of Trichoderma spp». DYNA 88 (218):224-29. https://doi.org/10.15446/dyna.v88n218.92420.

Harvard

Ramirez Olier, J. P., Gómez-Berrio, H. D. ., Trujillo-Salazar, J. A. ., Delgado-Gómez, D. L. ., Rivera-Zuleta, C. S. . y Rocío Botero, L. . (2021) «In vitro evaluation the toxicity in mixture of glyphosate and methyl metsulfuron against strains of Trichoderma spp», DYNA, 88(218), pp. 224–229. doi: 10.15446/dyna.v88n218.92420.

MLA

Ramirez Olier, J. P., H. D. . Gómez-Berrio, J. A. . Trujillo-Salazar, D. L. . Delgado-Gómez, C. S. . Rivera-Zuleta, y L. . Rocío Botero. «In vitro evaluation the toxicity in mixture of glyphosate and methyl metsulfuron against strains of Trichoderma spp». DYNA, vol. 88, n.º 218, julio de 2021, pp. 224-9, doi:10.15446/dyna.v88n218.92420.

Turabian

Ramirez Olier, Johana Patricia, Hernán David Gómez-Berrio, Juan Alberto Trujillo-Salazar, Dora Luz Delgado-Gómez, Claudia Sirley Rivera-Zuleta, y Liliana Rocío Botero. «In vitro evaluation the toxicity in mixture of glyphosate and methyl metsulfuron against strains of Trichoderma spp». DYNA 88, no. 218 (julio 28, 2021): 224–229. Accedido marzo 7, 2026. https://revistas.unal.edu.co/index.php/dyna/article/view/92420.

Vancouver

1.
Ramirez Olier JP, Gómez-Berrio HD, Trujillo-Salazar JA, Delgado-Gómez DL, Rivera-Zuleta CS, Rocío Botero L. In vitro evaluation the toxicity in mixture of glyphosate and methyl metsulfuron against strains of Trichoderma spp. DYNA [Internet]. 28 de julio de 2021 [citado 7 de marzo de 2026];88(218):224-9. Disponible en: https://revistas.unal.edu.co/index.php/dyna/article/view/92420

Descargar cita

CrossRef Cited-by

CrossRef citations5

1. Deepika Bamal, Anil Duhan, Ajay Pal, Ravi Kumar Beniwal, Priyanka Kumawat, Sachin Dhanda, Ankit Goyat, Virender Singh Hooda, Rajpaul Yadav. (2024). Herbicide risks to non-target species and the environment: A review. Environmental Chemistry Letters, 22(6), p.2977. https://doi.org/10.1007/s10311-024-01773-9.

2. Carlos Andrés Dodino-Gutiérrez, Jessica Rodríguez-Escobar. (2025). In vitro compatibility of Trichoderma asperellum with isotianil and pesticides of chemical and organic origin. Revista Colombiana de Ciencias Hortícolas, 19(1), p.1. https://doi.org/10.19053/uptc.24223719.18521.

3. Lindomar Canuto da Silva, Amanda Flausino de Faria, Rafaela Araújo Guimarães, Muhammad Siddique Afridi, Flavio Henrique Vasconcelos de Medeiros, Fernanda Carvalho Lopes de Medeiros. (2024). How can an in vitro incompatibility of Trichoderma-based products and herbicides impact the parasitism and control of white mold (Sclerotinia sclerotiorum (Lib.) De Bary)?. Crop Health, 2(1) https://doi.org/10.1007/s44297-024-00024-1.

4. Matheus Rakes, Maíra Chagas Morais, Michele Trombin de Souza, Mireli Trombin de Souza, Daniel Bernardi, Anderson Dionei Grützmacher, Leandro do Prado Ribeiro. (2026). Compatibility of insect growth regulators from natural and synthetic origins with mycoinsecticides used in the corn leafhopper management. Crop Protection, 199, p.107448. https://doi.org/10.1016/j.cropro.2025.107448.

5. Matheus Rakes, Maíra Chagas Morais, Maria Eduarda Sperotto, Odimar Zanuzo Zanardi, Gabriel Rodrigues Palma, Luana Floriano, Renato Zanella, Osmar Damian Prestes, Daniel Bernardi, Anderson Dionei Grützmacher, Leandro do Prado Ribeiro. (2026). Physicochemical, in vitro, and in vivo compatibilities of fungicides and mycoinsecticides used in maize crops. Biological Control, 213, p.105970. https://doi.org/10.1016/j.biocontrol.2026.105970.

Dimensions

PlumX

Visitas a la página del resumen del artículo

616

Descargas

Los datos de descargas todavía no están disponibles.