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Effect of the liquid-to-powder ratio on physicochemical properties of calcium phosphate cements and of these properties over Biofilm thickness of adhered Staphylococcus Aureus
Efecto de la relación líquido a polvo en las propiedades fisicoquímicas de cementos de fosfato de calcio y de estos sobre el espesor de Biofilm de Staphylococcus Aureus adherido
DOI:
https://doi.org/10.15446/dyna.v88n219.93812Palabras clave:
calcium phosphate cement, tricalcium phosphate, Staphylococcus aureus, biofilm thickness, specific surface area, Extracellular polysaccharide substances (en)Cemento de fosfato de calcio, fosfato tricálcico, Staphylococcus aureus, espesor biofilm, área superficial específica, subastancias polisacáridas extracelulares. (es)
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Tricalcium phosphate (TCP) synthetized by high temperature solid state reaction at 1400°C and three derived calcium phosphate cements (CPCs) prepared at liquid to powder (L/P) ratios of 0.33, 0.44 and 0.55 ml/g, respectively, were physicochemically characterized. Calcium deficient hydroxyapatite crystals were identified by scanning electron microscopy on CPC, and differences in crystal sizes were observed at different L/P ratios. Also, the biofilm thickness of two Staphylococcus Aureus (S.aureus) strains grown for 24 hours on the three CPC are reported. A dependence of the biofilm thickness with the specific surface area (SSA) of CPC was identified. They are directly proportional for non-extracellular polysaccharide substances (EPS) producing S.aureus and inversely proportional for EPS producing S.aureus. Non-proportional behavior between the SSA and mechanical strength of the CPC was observed as L/P ratio increases.
Se caracterizaron fisicoquímicamente un fosfato tricálcico (FTC) sintetizado por reacción de estado sólido a alta temperatura (1400ºC) y tres cementos de fosfato de calcio (CFC) derivados del FTC con relaciones líquido a polvo (L/P) de 0.33, 0.44 y 0.55 ml/g, respectivamente. Fueron identificados cristales de hidroxiapatita deficiente en calcio por microscopía electrónica de barrido en los CFC y se observaron diferencias en el tamaño de los cristales según la relación L/P. Además, se reporta el espesor de biofilm de dos cepas de Staphylococcus Aureus (S.aureus) cultivadas por 24 horas en los tres CFC. Se identificó una dependencia del espesor de biofilm con el área superficial específica (ASE) de los CFC: es directamente proporcional si el S.aureus no es productor de substancias polisacáridas extracelulares (SPE) e inversamente proporcional si lo es. Se identificó un comportamiento no proporcional entre la ASE y el esfuerzo mecánico de los CFC al incrementar la relación L/P.
Referencias
Rennert-May, E., Bush, K., Vickers, D. and Smith, S., Use of a provincial surveillance system to characterize postoperative surgical site infections after primary hip and knee arthroplasty in Alberta, Canada. American Journal of Infection Control,44(11), pp. 1310-1314, 2016. DOI: 10.1016/j.matchemphys.2008.10.020.
Uskokovic, V., Nanostructured platforms for the sustained and local delivery of antibiotics in the treatment of osteomyelitis. Critical Reviews in Therapeutic Drug Carrier Systems, 32(1), pp. 1-59, 2015. DOI: 10.1615/CritRevTherDrugCarrierSyst.2014010920.
Sadowska, J.M., Genoud, K.J., Kelly, D.J. and O’Brien, F.J., Bone biomaterials for overcoming antimicrobial resistance: Advances in non-antibiotic antimicrobial approaches for regeneration of infected osseous tissue. Materials Today, 46, pp. 136-154, 2021. DOI: 10.1016/j.mattod.2020.12.018.
Dorozhkin, S.V., Calcium orthophosphate bioceramics. Ceramics International, 41, pp. 13913-13966, 2015. DOI: 10.1016/j.ceramint.2015.08.004.
Canillas, M., Pena, P., Aza, A. De. and Rodríguez, M.A., Calcium phosphates for biomedical applications. Boletín de la Sociedad Española de Cerámica y Vidrio, 6, pp. 1-22, 2017. DOI: 10.1016/j.bsecv.2017.05.001.
Mensah, L.M. and Love, B.J., A meta-analysis of bone cement mediated antibiotic release: Overkill, but a viable approach to eradicate osteomyelitis and other infections tied to open procedures. Materials Science and Engineering: C, 123, art. 111999, 2021. DOI: 10.1016/j.msec.2021.111999.
Zhang, J., Liu, W., Schnitzler, V., Tancret, F. and Bouler, J.-M.M., Calcium phosphate cements for bone substitution: Chemistry, handling and mechanical properties. Acta Biomaterialia, 10(3), pp. 1035-1049, 2014. DOI: 10.1016/j.actbio.2013.11.001.
Canal, C. and Ginebra, M.P., Fibre-reinforced calcium phosphate cements: a review. Journal of the Mechanical Behavior of Biomedical Materials, 4(8), pp. 1658-1671, 2011. DOI: 10.1016/j.jmbbm.2011.06.023.
Gusnaniar, N., Sjollema, J., Nuryastuti, T., Peterson, B.W., van de Belt-Gritter, B., de Jong, E.D., et al., Structural changes in S. epidermidis biofilms after transmission between stainless steel surfaces. Biofouling, 33(9), pp. 1-10, 2017. DOI: 10.1080/08927014.2017.1360870.
Billings, N., Birjiniuk, A., Samad, T.S., Doyle, P.S. and Ribbeck, K., Material properties of biofilms—a review of methods for understanding permeability and mechanics. Reports on Progress in Physics, 78(3), art. 036601, 2015. DOI: 10.1088/0034-4885/78/3/036601.
Peterson, B.W., Mei, H.C. van der., Sjollema, J., Busscher, H.J. and Sharma, P.K., A distinguishable role of eDNA in the viscoelastic relaxation of biofilm. MBIO, 4(5), pp. 1-7, 2013. DOI: 10.1128/mBio.00497-13.
Silva, V., Miranda, C., Bezerra, M., Antão, H.S., Guimarães, J., Prada, J., et al., Anti-biofilm activity of dalbavancin against methicillin-resistant Staphylococcus aureus (MRSA) isolated from human bone infection. Journal of Chemotherapy, pp. 1-6, 2021. DOI: 10.1080/1120009X.2021.1911518.
Birt, M.C., Anderson, D.W., Bruce Toby, E. and Wang, J., Osteomyelitis: Recent advances in pathophysiology and therapeutic strategies. Journal of Orthopaedics, 14 (1), pp. 45-52, 2017. DOI: 10.1016/j.jor.2016.10.004.
Furustrand-Tafin, U., Betrisey, B., Bohner, M., Ilchmann, T., Trampuz, A. and Clauss, M., Staphylococcal biofilm formation on the surface of three different calcium phosphate bone grafts: a qualitative and quantitative in vivo analysis. Journal of Materials Science: Materials in Medicine, 26(3), pp. 130, 2015. DOI: 10.1007/s10856-015-5467-6.
Sharma, P., Rozenbaum, R.T., Woudstra, W., de Jong, E.D., van der Mei, H.C., Busscher, H.J., et al., A constant depth film fermenter to grow microbial biofilms. Protocol Exchange, (March), pp 1-21, 2017. DOI: 10.1038/protex.2017.024.
Wagner, M. and Horn, H., Optical coherence tomography in biofilm research: a comprehensive review. Biotechnology and Bioengineering, 114(7), pp. 1386-402, 2017. DOI: 10.1002/bit.26283.
Espanol, M., Perez, R.A., Montufar, E.B., Marichal, C., Sacco, A. and Ginebra, M.P., Intrinsic porosity of calcium phosphate cements and its significance for drug delivery and tissue engineering applications. Acta Biomaterialia, 5(7), pp. 2752-62, 2009. DOI: 10.1016/j.actbio.2009.03.011.
Paknahad, A., Kucko, N.W., Leeuwenburgh, S.C.G. and Sluys, L.J., Experimental and numerical analysis on bending and tensile failure behavior of calcium phosphate cements. Journal of the Mechanical Behavior of Biomedical Materials, 103(November), art 103565, 2020. DOI: 10.1016/j.jmbbm.2019.103565.
Pastorino, D., Canal, C. and Ginebra, M.-P., Drug delivery from injectable calcium phosphate foams by tailoring the macroporosity-drug interaction. Acta Biomaterialia, 12, pp. 250-259, 2015. DOI: 10.1016/j.actbio.2014.10.031.
Rasyid, H.N., Van Der Mei, H.C., Frijlink, H.W., Soegijoko, S., van Horn, J.R., Busscher, H.J., et al., Concepts for increasing gentamicin release from handmade bone cement beads. Acta Orthopaedica, 80(5), pp. 508-513, 2009. DOI: 10.3109/17453670903389782.
Carrodeguas, R.G., De Aza, A.H., Turrillas, X., Pena, P. and De Aza, S., New approach to the β→α polymorphic transformation in magnesium-substituted tricalcium phosphate and its practical implications. Journal of the American Ceramic Society, 91(4), pp. 1281-1286, 2008. DOI: 10.1111/j.1551-2916.2008.02294.x.
García-Páez, I.H., Carrodeguas, R.G., De Aza, A.H., Baudín, C. and Pena, P., Effect of Mg and Si co-substitution on microstructure and strength of tricalcium phosphate ceramics. Journal of the Mechanical Behavior of Biomedical Materials, 30C, pp. 1-15, 2013. DOI: 10.1016/j.jmbbm.2013.10.011.
Torres, P.M.C.C., Abrantes, J.C.C.C., Kaushal, A., Pina, S., Döbelin, N., Bohner, M., et al., Influence of Mg-doping, calcium pyrophosphate impurities and cooling rate on the allotropic α↔β-tricalcium phosphate phase transformations. Journal of the European Ceramic Society, 36(3), pp. 817-827, 2016. DOI: 10.1016/j.jeurceramsoc.2015.09.037.
O’Hara, R., Buchanan, F. and Dunne, N., Injectable calcium phosphate cements for spinal bone repair. Biomaterials for Bone Regeneration, Elsevier, 2014, pp. 26-61.
Loca, D., Sokolova, M., Locs, J., Smirnova, A. and Irbe, Z., Calcium phosphate bone cements for local vancomycin delivery. Materials Science and Engineering: C, 49, pp. 106-13, 2015. DOI: 10.1016/j.msec.2014.12.075.
Montufar, E.B., Maazouz, Y. and Ginebra, M.P., Relevance of the setting reaction to the injectability of tricalcium phosphate pastes. Acta Biomaterialia, 9(4), pp. 6188-6198, 2013. DOI: 10.1016/j.actbio.2012.11.028.
Ginebra, M.P., Driessens, F.C.M. and Planell, J.A., Effect of the particle size on the micro and nanostructural features of a calcium phosphate cement: a kinetic analysis. Biomaterials, 25(17), pp. 3453-62, 2004. DOI: 10.1016/j.biomaterials.2003.10.049.
O’Neill, R., McCarthy, H.O., Montufar, E.B., Ginebra, M.P., Wilson, D.I., Lennon, A., et al., Critical review: injectability of calcium phosphate pastes and cements. Acta Biomaterialia, 50, pp. 1-19, 2017. DOI: 10.1016/j.actbio.2016.11.019.
Chen, W.-C., Lin, J.-H.C. and Ju, C.-P., Transmission electron microscopic study on setting mechanism of tetracalcium phosphate/dicalcium phosphate anhydrous-based calcium phosphate cement. Journal of Biomedical Materials Research. Part A, 64, pp. 664-671, 2003. DOI: 10.1002/jbm.a.10250.
Li, J., Xu, W., Lin, X., Cao, F., Yang, J., Li, L., et al., A Ca-deficient a-deficient hydroxyapatite (CDHA)/MgF2 bi-layer coating with unique nano-scale topography on biodegradable high-purity Mg. Colloids and Surfaces B: Biointerfaces, 190(November), art. 110911, 2020. DOI: 10.1016/j.colsurfb.2020.110911.
Pastorino, D., Canal, C. and Ginebra, M.-P., Multiple characterization study on porosity and pore structure of calcium phosphate cements. Acta Biomaterialia, 28, pp. 205-214, 2015. DOI: 10.1016/j.actbio.2015.09.017.
Canal, C., Pastorino, D., Mestres, G., Schuler, P. and Ginebra, M.P., Relevance of microstructure for the early antibiotic release of fresh and pre-set calcium phosphate cements. Acta Biomaterialia, 9(9), pp. 8403-8412, 2013. DOI: 10.1016/j.actbio.2013.05.016.
Burtseva, O., Baulina, O., Zaytseva, A., Fedorenko, T., Chekanov, K. and Lobakova, E., In vitro biofilm formation by bioluminescent bacteria isolated from the marine fish Gut. Microbial Ecology, 81(4), pp. 932-940, 2021. DOI: 10.1007/s00248-020-01652-0.
Gusnaniar, N., Sjollema, J., Jong, E.D., Woudstra, W., de Vries, J., Nuryastuti, T., et al., Influence of biofilm lubricity on shear-induced transmission of staphylococcal biofilms from stainless steel to silicone rubber. Microbial Biotechnology, 10(6), pp. 1744-1752, 2017. DOI: 10.1111/1751-7915.12798.
Ren, Z., Kim, D., Paula, A.J., Hwang, G., Liu, Y., Li, J., et al., Dual-targeting approach degrades biofilm matrix and enhances bacterial killing. Journal of Dental Research, 98(3), pp. 322-330, 2019. DOI: 10.1177/0022034518818480.
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