Microencapsulation of aroma compounds produced by Pichia fermentans
Microencapsulación de compuestos aromáticos producidos por Pichia fermentans
DOI:
https://doi.org/10.15446/dyna.v89n220.97045Palabras clave:
Pichia fermentans, molasses, microencapsulation, sensory analysis (en)Pichia fermentans, melaza, microencapsulación, análisis sensorial (es)
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Aroma and fragrance compounds have broad applications in the food, cosmetic and pharmaceutical industries. However, these compounds are mainly produced by chemical synthesis. An alternative, sustainable and promising route for the natural production of flavour compounds is microbial transformation processes. In this work, we obtained the production of different aroma compounds in significant concentrations, such as isoamyl acetate, isoamyl alcohol, ethyl acetate and acetaldehyde, among others, from agribusiness by-products (sugarcane molasses) and using Pichia fermentans. These were subsequently concentrated by pervaporation and microencapsulation by spray drying using different encapsulating agents. The results showed that when these techniques were applied to two iced beverages, they produced an odour of fruity notes, especially those of banana. In addition, woody and alcoholic notes were identified by the panellists. Finally, the best encapsulating agents were dextrin (73.49 ppm) and the dextrin and maltodextrin mixture (72.58 ppm
Los compuestos aromáticos tienen amplias aplicaciones en las industrias alimentaria, cosmética y farmacéutica. Sin embargo, estos compuestos se producen principalmente por síntesis química. Una alternativa, sostenible y prometedora para la producción natural de compuestos aromáticos son los procesos fermentativos usando microrganismos. En este trabajo se obtuvo la producción de diferentes compuestos aromáticos en concentraciones significativas, como acetato de isoamilo, alcohol isoamílico, acetato de etilo y acetaldehído, entre otros, a partir de subproductos de la agroindustria (melaza de caña de azúcar), usando Pichia fermentans como inoculo. Los compuestos aromáticos se concentraron posteriormente por pervaporación y microencapsulación mediante secado por aspersión utilizando diferentes agentes encapsulantes. Se encontró que cuando estas técnicas se aplicaron a dos bebidas, produjeron un olor de notas afrutadas, especialmente las de plátano. Además, los panelistas identificaron notas amaderadas y alcohólicas. Finalmente, se encontró que los mejores agentes encapsulantes eran la dextrina (73,49 ppm) y la mezcla de dextrina y maltodextrina (72,58 ppm).
Referencias
Santos, P, Meireles, M.A.A. and Martínez, J., Production of isoamyl acetate by enzymatic reactions in batch and packed bed reactors with supercritical CO2, J. Supercrit. Fluids., 127(1), pp 71-80, 2017. DOI:https://doi.org/10.1016/j.supflu.2017.03.019.
Sánchez-Castañeda, A.K., Athès, V., Moussa, M., López-Miranda, J., Jesús, P-L., Soto-Cruz, N. et al., Modeling of isoamyl acetate production by fermentation with Pichia fermentans in an aerated system coupled to in situ extraction, Process Biochem., 65(1), pp. 11-20, 2018. DOI: https://doi.org/10.1016/j.procbio.2017.10.010.
Li, K. and Frost, J.W., Synthesis of vanillin from glucose, JACS., 120(1), pp. 10545-10546, 1998. DOI: https://doi.org/10.1021/ja9817747.
Scozzafava, A., Passaponti, M., Supuran, C.T. and Gülçin, İ., Carbonic anhydrase inhibitors: guaiacol and catechol derivatives effectively inhibit certain human carbonic anhydrase isoenzymes (hCA I, II, IX and XII), J. Enzyme Inhib. Med. Chem., 30(1), pp. 586-591, 2015. DOI: https://doi.org/10.3109/14756366.2014.956310
Lee, J. and Wendisch, V.F., Biotechnological production of aromatic compounds of the extended shikimate pathway from renewable biomass, J. Biotechnol., 257(1), pp. 211-221, 2017. DOI:https://doi.org/10.1016/j.jbiotec.2016.11.016.
Lubbers, R.J.M., Dilokpimol, A., Visser, J., Mäkelä, M.R., Hildén, K.S. and Vries, R.P. De., A comparison between the homocyclic aromatic metabolic pathways from plant-derived compounds by bacteria and fungi, Biotechnol. Adv., 37(1), art. 107396, 2019. DOI: https://doi.org/10.1016/j.biotechadv.2019.05.002.
Rodriguez, A., Martínez, J.A., Flores, N., Escalante, A., Gosset, G. and Bolivar, F., Engineering Escherichia coli to overproduce aromatic amino acids and derived compounds, Microb. Cell Fact., 13(1), pp. 1- 15, 2014. DOI: https://doi.org/10.1186/s12934-014-0126-z
Zhu, D., Zhang, P., Xie, C., Zhang, W., Sun, J., Qian, W.J., et al., Biotechnology for biofuels biodegradation of alkaline lignin by Bacillus ligniniphilus L1, Biotechnol. Biofuels., 10(1), pp. 1-14, 2017.DOI: https://doi.org/10.1186/s13068-017-0735-y.
Bruckner, C., Oreb, M., Kunze, G., Boles, E. and Tripp, J., An expanded enzyme toolbox for production of cis, cis -muconic acid and other shikimate pathway derivatives in Saccharomyces cerevisiae, FEMS Yeast Res., 18(1), pp. 1-12, 2018. DOI: https://doi.org/10.1093/femsyr/foy017.
Bluemke, W. and Schrader, J., Integrated bioprocess for enhanced production of natural flavors and fragrances by Ceratocystis moniliformis, Biomol. Eng., 17(1), pp. 137-142, 2001. DOI: https://doi.org/10.1016/S1389-0344(01)00072-7
Madene, A., Jacquot, M., Scher, J. and Desobry, S., Flavour encapsulation and controlled release - a review, Int. J. Food Sci., 41(1), pp. 1-21, 2006. DOI: https://doi.org/10.1111/j.1365-2621.2005.00980.x.
Huertas, R.P., Revisión: microencapsulación de alimentos, RevFacNalAgrMedellín., 63(1), pp. 5669-5684, 2011.
Sanchez-Reinoso, Z., Osorio, C. and Herrera, A., Effect of microencapsulation by spray drying on cocoa aroma compounds and physicochemical characterisation of microencapsulates, Powder Technol., 318(1), pp. 110-119, 2017. DOI: https://doi.org/10.1016/j.powtec.2017.05.040.
Saifullah, M., Mohammad, S., Rayhana, F., Rahman, R. and Vuong, Q., Micro and nano encapsulation, retention and controlled release of lfavor and aroma compounds: a critical review, Trends Food Sci Technol., 86(1), pp. 230-251, 2019. DOI: https://doi.org/10.1016/j.tifs.2019.02.030.
Ribeiro, A.M., Estevinho, B.N. and Rocha, F., Microencapsulation of polyphenols - The specific case of the microencapsulation of Sambucus Nigra L. extracts - A review, Trends Food Sci Technol., 1(1), pp. 1-14, 2019. DOI: https://doi.org/10.1016/j.tifs.2019.03.011.
Favaro-Trindade, C.S., Santana, A.S., Monterrey-Quintero, E.S., Trindade, M.A. andNetto, F.M., The use of spray drying technology to reduce bitter taste of casein hydrolysate, Food Hydrocoll., 24(1), pp. 336-340, 2009. DOI: https://doi.org/10.1016/j.foodhyd.2009.10.012.
Gharsallaoui, A. and Chambin, O., Applications of spray-drying in microencapsulation of food ingredients: an overview, Int. Food Res.J., 40(1), pp. 1107-1121, 2007. DOI: https://doi.org/10.1016/j.foodres.2007.07.004.
Ozkan, G., Franco, P., Marco, I. De, Xiao, J. and Capanoglu, E., A review of microencapsulation methods for food antioxidants: Principles, advantages, drawbacks and applications, Food Chem.,
(1), pp. 494-506, 2019. DOI: https://doi.org/10.1016/j.foodchem.2018.07.205.
Rossi, S., Medeiros, A., Weschenfelder, T., Scheer, A. and Soccol, C., Use of pervaporation process for the recovery of aroma compounds produced by P. fermentans in sugarcane molasses, Bioprocess Biosyst Eng., 40(1), pp. 959-967, 2017. DOI: https://doi.org/10.1007/s00449-017-17591.
Varian. NIst 98 MS Library Database, ver. 1.7.USA 1998. NIST Standard Reference Database 1A n.d.
Rocha, G., Fávaro-Trindade, C. and Grosso, C., Microencapsulation of lycopene by spray drying: Characterization, stability and application of microcapsules, Food Bioprod. Process., 90(1), pp. 37-42, 2012.DOI: https://doi.org/10.1016/j.fbp.2011.01.001.
Rodríguez, Y., López-Bello, N., Martinez, A. and Ley-Chong, N., Design stage of purification for obtaining isoamyl acetate from oil fusel, Tecnología Química, 37(1), pp. 405-416, 2017.
Salcedo, S., Arturo, C., Álvarez, M. y Crespo, C., Producción de ésteres volátiles a través de tecnología de fermentaciones utilizando residuos cítricos, Revista Con-Ciencia, 4(1), pp. 83-91, 2016.
Zare, M., Golmakani, M. and Niakousari, M., LWT - Food science and technology lipase synthesis of isoamyl acetate using different acyl donors: comparison of novel esterification techniques, LWT – Food Sci Technol., 101(1), pp. 214-219, 2019. DOI: https://doi.org/10.1016/j.lwt.2018.10.098.
Rodríguez-Alonso, P., Fern, I.C., Garabal, J.I. and Centeno, J.A., Characterization of yeasts isolated from artisanal short-ripened cows’ cheeses produced in Galicia (NW Spain), Food Microbiol 53(1), pp. 172-181, 2016. DOI: https://doi.org/10.1016/j.fm.2015.09.012.
Pereira, G., Neto, E., Thomaz, V., Bianchi, A., Lorenci, A. and Soccol, C., Conducting starter culture-controlled fermentations of coffee beans during on-farm wet processing: Growth, metabolic analyses and sensorial effects, Int. Food Res. J., 75(1), pp. 348-356, 2015. DOI: https://doi.org/10.1016/j.foodres.2015.06.027.
Freitas, I. De, Pinon, N., Maubois, J., Lortal, S. and Thierry, A., The addition of a cocktail of yeast species to Cantalet cheese changes bacterial survival and enhances aroma compound formation, International Journal of Food Microbiology, 129(1), pp. 37-42, 2009.DOI: https://doi.org/10.1016/j.ijfoodmicro.2008.10.026.
Yuso, N., Sangaran, S. and Rizal, S., Non-enzymatic synthesis route for production of isoamyl acetate in a solvent-free system using miniaturized intensi fi ed reactor, J. Environ. Chem. Eng., 103(1), pp. 103186, 2019. DOI: https://doi.org/10.1016/j.jece.2019.103186.
Kong, C., Li, A., Su, J., Wang, X., Chen, C. and Tao, Y., Flavor modi fi cation of dry red wine from Chinese spine grape by mixed fermentation with Pichia fermentans and S. cerevisiae, LWT – Food Sci Technol., 109(1), pp. 83-92, 2019. DOI: https://doi.org/10.1016/j.lwt.2019.03.101.
Jomori, M., Berno, N. and Kluge, R., Ethylene application after cold storage improves skin color of ‘valencia’ oranges, Rev Bras Frutic.,38(1), pp. 1-9, 2016. DOI:https://doi.org/10.1590/0100-29452016636.
Virginia, F., Garruti, D., Dias, C., Cordenunsi, B. and Lajolo, M.,Influence of different banana cultivars on volatile compounds during ripening in cold storage, Int. Food Res. J., 49(1), pp. 626-633, 2012.DOI: https://doi.org/10.1016/j.foodres.2012.08.013.
Tressl, R. and Jennings, W., Production of volatile compounds in the Ripening Banana, J. Agric. Food Chem., 20(1), pp. 189-192, 1972.
Martins, I.M., Barreiro, M.F., Coelho, M. and Rodrigues, A.E.,Microencapsulation of essential oils with biodegradable polymeric carriers for cosmetic applications, Chem Eng J., 245(1), pp. 191-200,2014. DOI: https://doi.org/10.1016/j.cej.2014.02.024.
Costa, S.S., Machado B., Martin, A., Bagnara, F., Regadalli, S. and Alves, A., Drying by spray drying in the food industry: Microencapsulation, process parameters and main carriers used, Afr. J. Food Sci., 9(1), pp. 462-470, 2016. DOI: https://doi.org/10.5897/AJFS2015.1279.
Aburto, L., De Queiroz, D. and Martucci, E., Microencapsulacãode óleo essencial de laranja, CiencTecnol Alime., 18(1), pp. 45-48, 1998.
Balasubramani, P., Palaniswamy, P.T., Visvanathan, R., Thirupathi, V., Subbarayan, A. and Maran, J., Microencapsulation of garlic oleoresin using maltodextrin as wall material by spray drying technology, Int. J. Biol. Macromol, 72(1), pp. 210-217, 2015. DOI:https://doi.org/10.1016/j.ijbiomac.2014.08.011.
Ascheri, D.P.R., Marquez, M. and Martucci, E.T., Microencapsulação de óleo essencial de laranja: seleção de material de parede 1. Ciênc Tecnol Aliment., 23(1), pp. 1-6, 2003.
Janiszewska, E., Jedli, A. and Witrowa-Rajchert, D., Effect of homogenization parameters on selected physical properties of lemon aroma powder. Food Bioprod. Process., 4(1), pp. 405-413, 2014. DOI:https://doi.org/10.1016/j.fbp.2014.05.006.
Osorio, C., Forero, D.P. and Carriazo, J.G., Characterisation and performance assessment of guava (Psidium guajava L.) microencapsulates obtained by spray-drying, Int. Food Res. J., 44(1),pp. 1174-1181, 2011. DOI: https://doi.org/10.1016/j.foodres.2010.09.007.
Pham-Hoang, B.N., Romero-Guido, C., Phan-Thi, H., Waché, Y.,Encapsulation in a natural, preformed, multi-component and complex capsule: yeast cells, Appl Microbiol Biotechnol., 97(1), pp. 6635-6645, 2013. DOI: https://doi.org/10.1007/s00253-013-5044-1.
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