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Protein profiles of follicle fluid of different sizes in cows and buffaloes
Perfiles de proteínas del fluido de folículos de diferente tamaño en vacas y búfalas
DOI:
https://doi.org/10.15446/rfnam.v77n1.108022Keywords:
Bos indicus, Bubalus bubalis, Composition, Follicular liquid, Proteins (en)Bos indicus, Bubalus bubalis, Composición , Líquido folicular, Proteínas (es)
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In vitro embryo production systems in buffaloes have great productive perspectives and opportunities for improvement. Among these, comparative studies with species with more significant advances in reproductive biotechnology have been developed. Accordingly, this work aimed to identify the differences in the electrophoretic profiles of proteins in the follicular fluid (FF) of cows and buffaloes and their possible association with follicle size. FF was obtained at the central abattoir in Medellín (Antioquia), Colombia, from small (<7 mm) and large (>7 mm) follicles from the ovaries of 25 cows (Bos indicus) and 20 buffaloes (Bubalus bubalis). The total protein content of the FF was quantified and subsequently depleted of albumin and immunoglobulins. Samples were subjected to denaturing electrophoresis in sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE) to determine the electrophoretic profiles using a photodocumenter. The values obtained for the relative amount of each band were compared between species and follicle sizes using the Mann-Whitney test. The results showed no significant differences in total protein concentration between the different follicle sizes and species. Further, 72.6% of the FF proteins are immunoglobulins and albumin. The profiles of small follicles (<7mm) in cows presented 19 bands and 11 in buffaloes. The molecular weight range of the bands detected was between 5 and 250 kDa. Quantitative differences of the proteins in the follicular fluids evaluated were identified. The information obtained may contribute to elucidating the physiological differences between large and small follicles but does not explain the differences between species.
Los sistemas de producción de embriones in vitro de búfalo tienen grandes perspectivas productivas y oportunidades de mejora. Entre ellas, el desarrollo de estudios comparativos con especies en las que existen mayores avances en esta biotecnología reproductiva. El objetivo del presente trabajo fue identificar diferencias en los perfiles electroforéticos del fluido folicular (FF) de diferentes tamaños en vacas y búfalas. Se obtuvo FF de folículos pequeños (<7 mm) y grandes (>7 mm), provenientes de ovarios de 25 vacas (Bos indicus) y 20 búfalas (Bubalus bubalis) colectados en la central de faenado de Medellín (Antioquia), Colombia. El contenido de proteína total del FF fue cuantificado y posteriormente se sometió a un proceso de depleción de albúmina e inmunoglobulinas. Para determinar los perfiles electroforéticos a través del análisis en un fotodocumentador, las muestras se sometieron a electroforesis desnaturalizante en geles de poliacrilamida y dodecilsulfato sódico (SDSPAGE). Se compararon los valores obtenidos de la cantidad relativa de cada banda entre especies y tamaños de folículos, mediante la prueba de Mann-Whitney. Los resultados evidenciaron que no hubo diferencias significativas en la concentración de proteína total entre los diferentes tamaños de folículo y las especies. Además, 72,6% de las proteínas del FF evaluado son inmunoglobulinas y albúmina. Los perfiles de los folículos pequeños (<7mm) presentaron 19 bandas en las vacas y 11 en las búfalas. Las bandas detectadas estuvieron en un rango de peso molecular entre 5 y 250 kDa. Se identificaron diferencias cuantitativas de las proteínas en los fluidos foliculares evaluados. La información obtenida puede contribuir a elucidar las diferencias fisiológicas entre folículos grandes y pequeños, pero no explica las diferencias entre especies.
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