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Hydrogen production by dark fermentation from by-products of coffee wet processing and other organic wastes
Producción de hidrógeno por fermentación oscura a partir de subproductos del beneficio húmedo del café y otros residuos orgánicos
DOI:
https://doi.org/10.15446/rfnam.v78n3.116340Keywords:
Biohydrogen, Co-digestion, Coffee pulp, Sustainable energy, Waste coffee water (en)Biohidrógeno, Co-digestión, Pulpa de café, Energía sostenible, Aguas residuales de café (es)
Wet coffee processing generates liquid and solid residues with a high organic load, which constitute a significant environmental problem in producing regions such as Pitalito, Huila (Colombia). This study evaluated hydrogen production by dark fermentation (DF) from first coffee wash water (FWCW) in co-digestion with vegetable waste (VW), sugarcane juice (SCJ), and coffee pulp (CP), without thermal pretreatments or external inoculation. The assays were carried out in a 35 L batch bioreactor under three treatments with different proportions (% v/v): L1 (18:25:5:5:14), L2 (18:48:0:0:35), and L3 (18:68:0:0:14), corresponding to VW, FWCW, CP, SCJ, and water, respectively. Treatment L1 reached the highest cumulative H2 production (70.03±2.65 L), as well as the best substrate volume yield (2.00±0.08 L H2 Lsubstrate-1) and H2 content (43.99±3.89%). According to the modified Gompertz model, L1 also presented the highest average production rate (2.70±0.82 L H2 h-1) and lag phase time of 24±6.93 h. The Wilcoxon test evidenced significant differences (P=0.05) in cumulative hydrogen production between L1 and L3, confirming the influence of substrate composition on the process. These results highlight that co-digestion of FWCW and VW represents a viable alternative for valorizing the byproducts generated in the wet coffee processing through hydrogen production.
El procesamiento húmedo del café genera residuos líquidos y sólidos con alta carga orgánica, los cuales constituyen una problemática ambiental significativa en regiones productoras como Pitalito, Huila (Colombia). Este estudio evaluó la producción de hidrógeno por fermentación oscura (DF) a partir de aguas del primer lavado de café (FWCW) en co-digestión con residuos vegetales (VW), jugo de caña (SCJ) y pulpa de café (CP), sin pretratamientos térmicos ni inoculación externa. Los ensayos se realizaron en un biorreactor batch de 35 L, bajo tres tratamientos con diferentes proporciones (% v/v): L1 (18:25:5:5:14), L2 (18:48:0:0:35) y L3 (18:68:0:0:14), correspondientes a VW, FWCW, CP, SCJ y agua, respectivamente. El tratamiento L1 alcanzó la mayor producción acumulada de H2 (70,03±2,65 L), así como el mejor rendimiento por volumen de sustrato (2,00±0,08 L H2 Lsustrato-1) y un contenido de H2 (43,99±3,89%). De acuerdo con el modelo de Gomperz modificado, L1 también presentó la mayor tasa media de producción (2,70±0,82 L H2 h-1) y un tiempo de fase Lag de 24±6,93 h. La prueba de Wilcoxon evidenció diferencias significativas (P=0,05) en la producción acumulada de hidrógeno entre L1 y L3, confirmando la influencia de la composición del substrato en el proceso. Estos resultados destacan que la co-digestión de las FWCW y VW representa una alternativa viable para valorizar los subproductos generados en la vía húmeda del café mediante la generación de hidrógeno.
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