Correo Electrónico
DNINFOA - SIA
Bibliotecas
Convocatorias
Identidad U.N.
Published
Woody Plant Medium optimizes in vitro germination and development of Calycophyllum spruceanum
Woody Plant Medium optimiza la germinación y el desarrollo de Calycophyllum spruceanum in vitro
DOI:
https://doi.org/10.15446/rfnam.v78n3.119370Keywords:
Agar, Capirona, Culture media, Environmental restoration, Rubiaceae (en)Agar, Capirona, medio de cultivo, Restauración ambiental, Rubiaceae (es)
Climate change and biodiversity loss pose critical threats to ecosystems, making reforestation with native species an attractive mitigation strategy. However, seedling production systems need to be optimized. This study aimed to evaluate the effect of different culture media and concentrations on the germination and in vitro morphological development of Calycophyllum spruceanum. A completely randomized design with five treatments was used: 100% Murashige and Skoog (MS), 50% MS, 100% Woody Plant Medium (WPM), 50% WPM, and a control treatment (agar), with seven replicates per treatment. Germination parameters were evaluated for 21 days, and morphological development at 70 days. The main results showed that WPM and agar obtained the highest germination percentages (73.33 and 75.24%, respectively) and germination rate (2.02 and 2.32 seeds per day, respectively), while WPM stood out in terms of final germination time. In morphological development, WPM recorded the highest values for number of leaves (8.18), nodes (5.25), plant height (16.83 cm), and fresh and dry biomass (147.27 and 16.07 mg), compared to the other treatments. In addition, principal component analysis showed that WPM was associated with germination and development variables, with significant correlations. In conclusion, the WPM medium optimized germination and promoted the development of healthy and homogeneous seedlings, positioning it as an efficient alternative for propagation, ecological restoration, and native species conservation programs.
El cambio climático y la pérdida de biodiversidad representan amenazas críticas para los ecosistemas, por lo que la reforestación con especies nativas surge como una alternativa mitigadora. Sin embargo, se requiere optimizar los sistemas de producción de plántulas. El objetivo de este estudio fue evaluar el efecto de diferentes medios de cultivo y concentraciones en la germinación y el desarrollo morfológico in vitro de Calycophyllum spruceanum. Se utilizó un diseño completamente al azar con cinco tratamientos: 100% Murashige and Skoog (MS), 50% MS, 100% WPM, 50% WPM y un tratamiento control (agar), con siete repeticiones por tratamiento. Se evaluaron parámetros de germinación durante 21 días y desarrollo morfológico a los 70 días. Los principales resultados demostraron que WPM y agar obtuvieron los mayores porcentajes de germinación (73,33 y 75,24%), índice de velocidad de germinación (2,02 y 2,32 semillas por día), mientras en el tiempo de germinación final, el WPM destacó. En el desarrollo morfológico, WPM registró los valores más altos de número de hojas (8,18), nodos (5,25), altura de planta (16,83 cm) y biomasa fresca y seca (147,27 y 16,07 mg), en comparación con los demás tratamientos. Además, el análisis de componentes principales mostró que WPM se asoció a las variables de germinación y desarrollo, con correlaciones significativas. En conclusión, el medio WPM optimizó la germinación y favoreció el desarrollo de plántulas saludables y homogéneas, lo que lo posiciona como una alternativa eficiente para programas de propagación, restauración ecológica y conservación de especies nativas.
References
Bewley JD and Black M (1994) Seeds. Physiology of development and germination. Second edition. Springer, New York. 445 p. https://doi.org/10.1007/978-1-4899-1002-8
Blankinship LA, Gillaspie S and Aboul-Enein BH (2024) Highlighting the importance of biodiversity conservation through the Holy Qur’an. Conservation Biology 39: e14309. https://doi.org/10.1111/cobi.14309
Cabral-Miramontes JP, Chávez-Simental JA, Pulido-Díaz C et al (2022) In vitro propagation of apple tree from mature zygotic embryos. Revista Mexicana de Ciencias Agrícolas 13(4): 603–616. https://doi.org/10.29312/remexca.v13i4.2164
da Silva YKR, Martins GC, Guedes RS et al (2024) Native plant species as an alternative to rehabilitate iron ore waste piles in Carajás Mineral Province, Brazil. Communications in Soil Science and Plant Analysis 55(11): 1579–1592. https://doi.org/10.1080/00103624.2024.2321934
Das J, Jha S and Goyal MK (2020) On the relationship of climatic and monsoon teleconnections with monthly precipitation over meteorologically homogenous regions in India: Wavelet and global coherence approaches. Atmospheric Research 238: 104889. https://doi.org/10.1016/j.atmosres.2020.104889
Delgado-Paredes GE, Vásquez-Díaz C, Esquerre-Ibañez B et al (2023) Germinación de semillas, micropropagación y conservación de germoplasma in vitro de Cedrela odorata (Meliaceae) en el norte del Perú. Bosque (Valdivia) 44(1): 97–109. https://doi.org/10.4067/s0717-92002023000100097
Figueiredo FDA, Vale PAA, Mesquita AGG et al (2014) In vitro micropropagation and callus induction of Mulateiro (Calycophyllum spruceanum) seeds collected from the Amazon Basin. The International Journal of Science and Technoledge 2(3): 1–3.
Gonzales-Alvarado AC, Mori-Vásquez JA, Tuisima Coral LL et al (2022) Influencia de la desinfección, medios de cultivo y fitohormonas en el desarrollo morfogénico in vitro de germoplasma de Guazuma crinita Mart. Folia Amazónica 31(1): 57–66. https://doi.org/10.24841/fa.v31i1.572
Gonzales-Alvarado AC and Cardoso JC (2024) Development, chlorophyll content, and nutrient accumulation in in vitro shoots of Melaleuca alternifolia under light wavelengths and 6-BAP. Plants 13(20): 2842. https://doi.org/10.3390/plants13202842
Gonzales-Alvarado AC, Tuanama Pinchi RR, Arévalo-Salles CC et al (2024) Respuesta germinativa y desarrollo fisiológico in vitro de Guazuma crinita Mart. a partir de semillas sexuales. Acta Agronómica 71(1): 16–24.
Guerra-Arévalo WF, Cercado-Delgado JR, Espinoza-García HF et al (2024) Emergence, growth, and quality of Calycophyllum spruceanum plants produced in different containers and substrates. Agrosystems, Geosciences and Environment 8: e70002. https://doi.org/10.1002/agg2.70002
Hardarani N, Zahra SN, Wahdah R et al (2023) Respon Eksplan Buku Durian Lahung (Durio dulcis) terhadap konsentrasi BAP (Benzil Amino Purin) pada media WPM (Woody Plant Medium). Daun: Jurnal Ilmiah Pertanian dan Kehutanan 10(2): 235–247. https://doi.org/10.33084/daun.v10i2.6091
Indacochea B, Parrales J, Hernández A et al (2018) Evaluación de medios de cultivo in vitro para especies forestales nativas en peligro de extinción en Ecuador. Agronomía Costarricense 42(1). https://doi.org/10.15517/rac.v42i1.32203
Kirillov V, Pathak A, Patel SR et al (2024) An efficient micropropagation protocol for an endangered tree species Aflatunia ulmifolia (Franch.) Vassilcz. In vitro Cellular and Developmental Biology - Plant 60: 28-38. https://doi.org/10.1007/s11627-023-10392-y
Krasnoperova V and Bukharina I (2020) The study into the method of culture in vitro as a method of vegetative propagation of coniferous trees. Russian Agricultural Sciences 46:19–22. https://doi.org/10.3103/S1068367420010061
Lima LM, Silva WL, Souza JC et al (2024) In vitro cultivation of Vismia japurensis: Isolation of the new anthrone 1,8,10-trihydroxy3,10 dimethyl-9(10H)-anthracenone. Anais da Academia Brasileira de Ciências 96: e20230456. https://doi.org/10.1590/0001-3765202420230456
Marwein D, Vijayan D and Asosii AM (2024) Optimized in vitro propagation, acclimatization and reintroduction of endemic Rhododendron inaequale Hutch. from northeast India. Asian Journal of Biotechnology and Bioresource Technology 10(3): 51–59. https://doi.org/10.9734/ajb2t/2024/v10i3211
Marwein D and Vijayan D (2024) Influence of culture media and growth hormones on in vitro propagation of Adinandra griffithii Dyer., a critically endangered and endemic plant from northeast India. Vegetos 38: 1047–1054. https://doi.org/10.1007/s42535-024-00900-9
Meneses LS, Morillo LE and Vásquez-Castillo W (2022) In vitro propagation of Vaccinium floribundum Kunth from seeds: Promissory technology for mortiño accelerated production. Canadian Journal of Plant Science 102(1): 216–224. https://doi.org/10.1139/cjps-2020-0290
Mori-Vásquez JA, Panduro Tenazoa NM, Muñoz Fernandez VJ et al (2024) Efecto de la desinfección, el desarrollo foliar y las fitohormonas en la inducción de callos de Simarouba amara Aubl. Ciencia y Tecnología Agropecuaria 25(3): e3662. https://doi.org/10.21930/rcta.vol25_num3_art:3662
Munthali C, Kinoshita R, Onishi K et al (2022) A model nutrition control system in potato tissue culture and its influence on plant elemental composition. Plants 11(20): 2718. https://doi.org/10.3390/plants11202718
Olivas OO (2014) Desinfección de yemas axilares para su establecimiento in vitro de la Capirona (Calycophyllum spruceanum Benth). [Tesis de pregrado, Universidad Nacional Agraria de la Selva]. Repositorio Institucional UNAS.
Pereira L (2023) Chapter 3 - Algal polysaccharides. In Dominguez H, Pereira L, Kraan S (eds) Functional ingredients from algae for foods and nutraceuticals (Second edition). Woodhead Publishing, pp 151–212. https://doi.org/10.1016/B978-0-323-98819-3.00015-8
Saldaña CL, Rodriguez-Grados P, Chávez-Galarza JC et al (2022) Unlocking the complete chloroplast genome of a native tree species from the Amazon Basin, Capirona (Calycophyllum spruceanum, Rubiaceae), and its comparative analysis with other Ixoroideae species. Genes 13(1): 113. https://doi.org/10.3390/genes13010113
Santos AB, Ribeiro-Oliveira JP and Carvalho CM (2016) Sobre a botânica, a etnofarmacologia e a química de Calycophyllum spruceanum (Benth.) Hook. f. ex K. Schum. Revista Brasileira de Plantas Medicinais 18(1 suppl 1): 383–389. https://doi.org/10.1590/1983-084X/15_152
Stachevski TW, Franciscon L and Degenhardt-Goldbach J (2013) Efeito do meio de cultura na calogênese in vitro a partir de folhas de erva-mate. Pesquisa Florestal Brasileira 33: 339–342. https://doi.org/10.4336/2013.pfb.33.75.441
Sudheer WN, Praveen N, Al-Khayri JM et al (2022) Chapter 3 - Role of plant tissue culture medium components. In Rai AC, Kumar A, Modi A, Singh M (eds) Advances in plant tissue culture. Academic Press, pp 51–83. https://doi.org/10.1016/B978-0-323-90795-8.00012-6
Valladares F and Niinements Ü (2008) Shade tolerance, a key plant feature of complex nature and consequences. Annual Review of Ecology, Evolution, and Systematics 39(1): 237–257. https://doi.org/10.1146/annurev.ecolsys.39.110707.173506
Wei T, Simko V, Levy M et al (2024) corrplot: Visualization of a Correlation Matrix (Version 0.95) [Software]. https://cran.r-project.org/web/packages/corrplot/index.html
Wickham H (2016) Programming with ggplot2. In Wickham H (ed) Ggplot2: Elegant Graphics for Data Analysis. Springer International Publishing, pp 241–253. https://doi.org/10.1007/978-3-319-24277-4_12
Zhang H, Lee CKF, Law YK et al (2024) Integrating both restoration and regeneration potentials into the real-world forest restoration planning. SSRN. https://doi.org/10.2139/ssrn.4752980
How to Cite
APA
ACM
ACS
ABNT
Chicago
Harvard
IEEE
MLA
Turabian
Vancouver
Download Citation
License
Copyright (c) 2025 Revista Facultad Nacional de Agronomía Medellín
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The journal allows the author(s) to maintain the exploitation rights (copyright) of their articles without restrictions. The author(s) accept the distribution of their articles on the web and in paper support (25 copies per issue) under open access at local, regional, and international levels. The full paper will be included and disseminated through the Portal of Journals and Institutional Repository of the Universidad Nacional de Colombia, and in all the specialized databases that the journal considers pertinent for its indexation, to provide visibility and positioning to the article. All articles must comply with Colombian and international legislation, related to copyright.
Author Commitments
The author(s) undertake to assign the rights of printing and reprinting of the material published to the journal Revista Facultad Nacional de Agronomía Medellín. Any quotation of the articles published in the journal should be made given the respective credits to the journal and its content. In case content duplication of the journal or its partial or total publication in another language, there must be written permission of the Director.
Content Responsibility
The Faculty of Agricultural Sciences and the journal are not necessarily responsible or in solidarity with the concepts issued in the published articles, whose responsibility will be entirely the author or the authors.
