Physicochemical and Microbiological Characterization of Pasteurized Coyol Wine (Acrocomia aculeata) from Honduras
Caracterización fisicoquímica y microbiológica del vino de coyol pasteurizado (Acrocomia aculeata) de Honduras
DOI:
https://doi.org/10.15446/rfnam.v79.123640Keywords:
Acrocomia aculeata, Alcoholic fermentation, Fermented beverages, Food microbiology, Pasteurization (en)Acrocomia aculeata, Fermentación alcohólica, Bebidas fermentadas, Microbiología de los alimentos, Pasteurización (es)
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Fermented coyol sap (Acrocomia aculeata) is a traditional Honduran beverage with high cultural value and agro-industrial potential; however, its limited shelf life and microbiological stability restrict its commercialization. This study evaluated the effect of two thermal pasteurization treatments on the physicochemical, microbiological, and sensory stability of coyol wine during 28 days of storage bottles were stored in darkness at 30 °C. The beverage was pasteurized at 60 °C for 30 min (T1) and 75 °C for 10 min (T2), and pH, titratable acidity, soluble solids, ethanol content, and microbiological quality were evaluated. Initial pH values were 3.71 and 3.67 for T1 and T2, respectively, with a significant increase in T1 reaching 3.78 at day 28 (P≤0.05). Soluble solids showed no significant differences between treatments or during storage, remaining at 11 °Brix. Titratable acidity increased significantly in T1 from 2.25% at the beginning to 2.75 and 2.80% on days 21 and 28, respectively. Ethanol content reached maximum values on day 7 (14.0% (v/v) in T1 and 12.50% (v/v) in T2) and decreased by day 28 to 7.28 and 7.69% (v/v), respectively. Both treatments kept the values of hygiene and safety indicator microorganisms (Escherichia coli and total coliforms) below the detection limit (<10 CFU mL-1), demonstrating microbiological stability. Pasteurization at 75 °C for 10 min favored the preservation of coyol wine characteristics, representing a technological alternative for process standardization and agro-industrial valorization.
La savia fermentada de coyol (Acrocomia aculeata) es una bebida tradicional hondureña con alto valor cultural y potencial agroindustrial; sin embargo, su limitada vida útil y estabilidad microbiológica restringen su comercialización. Este estudio evaluó el efecto de dos tratamientos de pasteurización térmica sobre la estabilidad fisicoquímica, microbiológica y sensorial del vino de coyol durante 28 días de almacenamiento; las botellas se almacenaron en la oscuridad a 30 °C. La bebida fue pasteurizada a 60 °C durante 30 min (T1) y a 75 °C durante 10 min (T2), evaluándose pH, acidez titulable, sólidos solubles, contenido de etanol y calidad microbiológica. El pH inicial fue de 3.71 y 3.67 para T1 y T2, respectivamente, observándose un incremento significativo en T1 hasta 3.78 al día 28 (P≤0,05). Los sólidos solubles no presentaron diferencias significativas entre tratamientos ni durante el almacenamiento, manteniéndose en 11 °Brix. La acidez titulable aumentó significativamente en T1 desde 2,25% al inicio hasta 2,75 y 2,80% a los días 21 y 28. El contenido de etanol alcanzó valores máximos al día 7 (14,0% v/v en T1 y 12,50% v/v en T2) y disminuyó al día 28 hasta 7,28% y 7,69% v/v, respectivamente. Ambos tratamientos mantuvieron los valores de microorganismos indicadores de higiene e inocuidad (Escherichia coli y coliformes totales) por debajo del límite de detección (<10 UFC mL-1), evidenciando estabilidad microbiológica. La pasteurización a 75 °C durante 10 min favoreció la conservación de las características del vino de coyol, constituyendo una alternativa tecnológica para su estandarización y valorización agroindustrial.
References
Afolabi FT and Owoola AT (2019) Microbiological and Physicochemical Changes in Palm Wine Subjected to Spontaneous Fermentation During Storage. The International Journal of Biotechnology, 8(1):48–58. https://doi.org/10.18488/journal.57.2019.81.48.58
Andrade AC, Marinho JFU, de Souza AC et al (2020) Prebiotic potential of pulp and kernel cake from Jerivá (Syagrus romanzoffiana) and Macaúba palm fruits (Acrocomia aculeata). Food Research International 136:109595. https://doi.org/10.1016/j.foodres.2020.109595
Andrews WH and Hammack TS (2022) Food Sampling/Preparation of Sample Homogenate. In: Bacteriological Analytical Manual, April 2022. U.S. Food and Drug Administration. https://www.fda.gov/food/laboratory-methods-food/bam-chapter-1-food-samplingpreparation-sample-homogenate
Azizi-Lalabadi M, Moghaddam NR and Jafari SM (2023) Pasteurization in the food industry. In: Thermal Processing of Food Products by Steam and Hot Water. Elsevier, pp 247–273. https://doi.org/10.1016/B978-0-12-818616-9.00009-2
Costa LC, Batista NA, Cunha Neto A et al (2025) Fermented bocaiuva (Acrocomia aculeata) pulp: A focus on the survival of Lactiplantibacillus plantarum during freezing storage and simulated in vitro gastrointestinal conditions. Food Research International 220:117066. https://doi.org/10.1016/j.foodres.2025.117066
Cuvas-Limon RB, Nobre C, Cruz M et al (2021) Spontaneously fermented traditional beverages as a source of bioactive compounds: an overview. Critical Reviews in Food Science and Nutrition 61:2984–3006. https://doi.org/10.1080/10408398.2020.1791050
Das S and Tamang JP (2023) Fermentation Dynamics of Naturally Fermented Palm Beverages of West Bengal and Jharkhand in India. Fermentation, 9(3):301. https://doi.org/10.3390/fermentation9030301
de Lima NE, Carvalho AA, Meerow AW et al (2018) A review of the palm genus Acrocomia: Neotropical green gold. Organisms Diversity & Evolution 18:151–161. https://doi.org/10.1007/s13127-018-0362-x
de Matos Reis S, Mendes G da RL, Mesquita BMA de C et al (2021) Development of milk drink with whey fermented and acceptability by children and adolescents. Journal of Food Science and Technology 58:2847–2852. https://doi.org/10.1007/s13197-021-05003-w
Dilmetz BA, Brar G, Desire CT et al (2024) Performance of Different Saccharomyces Strains on Secondary Fermentation during the Production of Beer. Foods 13(16):2593. https://doi.org/10.3390/foods13162593
Ding J, Hou X, Li J et al (2025) The Effects of Pasteurization and Beer Type on the Functional Compounds and Flavor Substances in Beer. Beverages 11(3):63. https://doi.org/10.3390/beverages11030063
Feng P, Weagant SD, Grant MA and Burkhardt W (2020) BAM Chapter 4: Enumeration of Escherichia coli and the Coliform Bacteria. In: Bacteriological Analytical Manual, October 2020. U.S. Food and Drug Administration. https://www.fda.gov/food/laboratory-methods-food/bam-chapter-4-enumeration-escherichia-coli-and-coliform-bacteria
Hawaz H, Bottari B, Scazzina F and Carini E (2025) Eastern African traditional fermented foods and beverages: Advancements, challenges, and perspectives on food technology, nutrition, and safety. Comprehensive Reviews in Food Science and Food Safety 24(2): e70137. https://doi.org/10.1111/1541-4337.70137
He Y, Xie Z, Zhang H et al (2022) Oxidative Fermentation of Acetic Acid Bacteria and Its Products. Frontiers in Microbiology 13:879246. https://doi.org/10.3389/fmicb.2022.879246
Huang L, Ge Q and Peng B (2026) Yeast‐Derived Esters in Wine: Biosynthesis, Regulation, and Metabolic Engineering. Comprehensive Reviews in Food Science and Food Safety 25(1). https://doi.org/10.1111/1541-4337.70395
Karamoko D, Djeni NT, N’guessan KF et al (2012) The biochemical and microbiological quality of palm wine samples produced at different periods during tapping and changes which occured during their storage. Food Control 26(2):504–511. https://doi.org/10.1016/j.foodcont.2012.02.018
Khadka N, Acharya DR, Dangal A et al (2024) Study on the changes during the fermentation of the wine prepared from palm (Phoenix sylvestris) sap. Heliyon 10(15). https://doi.org/10.1016/j.heliyon.2024.e35799
Lasso García C, German Bermúdez FA, Vanden Berghe W et al (2024) Fermented beverages among indigenous Latin American societies. Frontiers in Sustainable Food Systems 8:1390162. https://doi.org/10.3389/fsufs.2024.1390162
Mandha J, Shumoy H, Matemu AO and Raes K (2023) Characterization of fruit juices and effect of pasteurization and storage conditions on their microbial, physicochemical, and nutritional quality. Food Bioscience 51:102335. https://doi.org/10.1016/j.fbio.2022.102335
Montanari C, Tabanelli G, Zamagna I et al (2019) Modeling of yeast thermal resistance and optimization of the pasteurization treatment applied to soft drinks. International Journal of Food Microbiology 301:1–8. https://doi.org/10.1016/j.ijfoodmicro.2019.04.006
Montoya-Arroyo A, Alfaro-Solís JD, Esquivel P et al (2021) Vitamin E profiles in Acrocomia aculeata from three regions in Costa Rica. Journal of Food Composition and Analysis 100:103936. https://doi.org/10.1016/j.jfca.2021.103936
Odo IA and George-Okafor UO (2021) Microbiota evaluation of four-month aged palm wine (Elaeis guneensis) from Ogba Umuosigide, Udenu L.G.A. Enugu state, Nigeria. International Journal of Advanced Academic Research 7:1–12. https://www.researchgate.net/publication/358119757
Petruzzi L, Campaniello D, Speranza B et al (2017) Thermal Treatments for Fruit and Vegetable Juices and Beverages: A Literature Overview. Comprehensive Reviews in Food Science and Food Safety 16(4):668–691. https://doi.org/10.1111/1541-4337.12270
Poláková K, Bobková A, Demianová A et al (2023) Quality Attributes and Sensory Acceptance of Different Botanical Coffee Co-Products. Foods 12(14):2675. https://doi.org/10.3390/foods12142675
Salar FJ, Díaz-Morcillo A, Fayos-Fernández J et al (2023) Microwave Treatment vs. Conventional Pasteurization: The Effect on Phytochemical and Microbiological Quality for Citrus–Maqui Beverages. Foods 13:101. https://doi.org/10.3390/foods13010101
Santiago-Urbina JA, Verdugo-Valdez AG and Ruiz-Terán F (2013) Physicochemical and microbiological changes during tapping of palm sap to produce an alcoholic beverage called “taberna”, which is produced in the south east of Mexico. Food Control 33:58–62. https://doi.org/10.1016/j.foodcont.2013.02.010
Seo H-B, Kim H-J, Lee O-K et al (2009) Measurement of ethanol concentration using solvent extraction and dichromate oxidation and its application to bioethanol production process. Journal of Industrial Microbiology & Biotechnology 36:285–292. https://doi.org/10.1007/s10295-008-0497-4
Tamang JP, Cotter PD, Endo A et al (2020) Fermented foods in a global age: East meets West. Comprehensive Reviews in Food Science and Food Safety 19:184–217. https://doi.org/10.1111/1541-4337.12520
Tournas V, Stack ME, Mislivec PB et al (2001) BAM Chapter 18: Yeasts, Molds, and Mycotoxins. In: Bacteriological Analytical Manual, April 2001. U.S. Food and Drug Administration. https://www.fda.gov/food/laboratory-methods-food/bam-chapter-18-yeasts-molds-and-mycotoxins
Zhang G, Ferguson M and Blodgett RJ (2025) BAM Chapter 3: Aerobic Plate Count. In: Bacteriological Analytical Manual, May 2025. U.S. Food and Drug Administration. https://www.fda.gov/food/laboratory-methods-food/bam-chapter-3-aerobic-plate-count
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