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Molecular detection of potato yellow vein virus (PYVV; Crinivirus flavisolani) in tomato tissues from Colombia
Detección molecular de potato yellow vein virus (PYVV; Crinivirus flavisolani) en tejidos de tomate de Colombia
DOI:
https://doi.org/10.15446/rfnam.v79.126098Keywords:
Closteroviridae, Seed-transmission, Solanum lycopersicum, RT-qPCR, Virus detection (en)Closteroviridae, Transmisión por semilla, Solanum lycopersicum, RT-Qpcr, Detección de virus (es)
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Potato yellow vein virus (PYVV; Crinivirus flavisolani) is a major limiting virus in potato production in Colombia and other South American countries. This virus is transmitted by both seed tubers and the whitefly Trialeurodes vaporariorum. While PYVV has also been reported in naranjilla, cape gooseberry, and tomato, its epidemiology in these solanaceous species remains poorly understood. Unlike potatoes, these crops are propagated by sexual seeds and are commercially distributed as seedlings. This study evaluated the presence of PYVV in tomato crops from various departments in Colombia using RT-qPCR with different tissues including leaves, fruit pericarp, seeds, and seedling shoots. The genomic identity of the virus was confirmed through high-throughput sequencing (HTS) of leaf samples from the Chonto tomato variety. A seed-to-seedling transmission assay was also conducted using surface-disinfected seeds cultured in vitro on MS medium. The virus was detected in 75% of fruit samples, 35% of leaf samples, and 40% of seedling shoot samples, but only in 10% of non-commercial seeds and none of the commercial seeds. HTS enabled assembly of the nearly complete viral genome with high sequencing depth. The in vitro assay indicated that PYVV has a low-frequency seed-to-seedling transmission in tomato, suggesting that disease management strategies should prioritize control of the whitefly vectors.
Potato yellow vein virus (PYVV; Crinivirus flavisolani) es uno de los virus más limitantes en cultivos de papa de Colombia y otros países suramericanos, siendo transmitido por tubérculos-semilla y por la mosca blanca Trialeurodes vaporariorum. Aunque este virus también se ha reportado infectando lulo, uchuva y tomate, poco se conoce sobre su epidemiología en estas solanáceas, que, a diferencia de la papa, se propagan por semilla sexual y por plántulas distribuidas comercialmente. En este estudio, mediante RT-qPCR se evaluó la presencia del PYVV en cultivos de tomate de diferentes departamentos de Colombia, utilizándose para su evaluación diferentes tejidos (i.e. foliar, pericarpo de frutos, semillas, brotes de plántulas). La identidad genómica del virus se confirmó por secuenciación de alto rendimiento (HTS) de muestras foliares de tomate variedad Chonto. En adición, se realizó un ensayo de transmisión semilla a plántula con semillas desinfectadas superficialmente y cultivadas bajo condiciones in vitro en medio MS. Los resultados indicaron la presencia del virus en el 75, 35 y 40%, de las muestras de frutos, foliares y brotes de plántulas, respectivamente, pero sólo en el 10% de las semillas no comerciales y en ninguna de las semillas comerciales. Mediante HTS se logró el ensamblaje del genoma casi completo del virus con altos niveles de profundidad. La prueba in vitro indicó que el PYVV presenta una transmisión de baja frecuencia de la semilla a la plántula en el tomate, por lo que el manejo de la enfermedad debe centrarse en el control de las moscas blancas.
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