Evaluation of the Antioxidant Properties and Aromatic Profile During Maturation of The Blackberry (Rubus glaucus Benth) and The Bilberry (Vaccinium meridionale Swartz)
Evaluación de las Propiedades Antioxidantes y el Perfil Aromático Durante la Maduración de Mora (Rubus glaucus Benth) y Agraz (Vaccinium meridionale Swartz)
Keywords:
Antioxidant activity, electronic nose, Folin-Ciocalteu, FRAP, TEAC (en)Actividad antioxidante, nariz electrónica, Folin- Ciocalteu, FRAP, TEAC (es)
The blackberry (Rubus glaucus Benth) and the bilberry (Vaccinium meridionale Swartz) are natural sources of antioxidants; they are known for their preventive role against degenerative diseases. In this study, the aromatic profile was evaluated using an electronic nose, including the antioxidant properties and the vitamin C, phenolic and anthocyanin contents during three stages of blackberry and bilberry ripening. A completely random statistical design was followed and the results presented differences in the aromatic profile: a higher anthocyanin content (1.59 mg of cyn-3-glu g-1 in the bilberry and 0.26 mg of cyn-3-glu g-1 in the blackberry) and total phenols (5.57 mg of caffeic acid g-1 bilberry and 2.68 mg caffeic acid g-1 blackberry). The behavior of the evaluated properties was independent in each of the fruits.
Los frutos como la mora (Rubus glaucus Benth) y el agraz (Vaccinium meridionale Swartz) son fuentes naturales de sustancias antioxidantes reconocidas por su papel preventivo en el desarrollo de enfermedades degenerativas. En este estudio se evaluó el perfil aromático por medio de nariz electrónica, las propiedades antioxidantes y el contenido de vitamina C, fenoles y antocianinas totales, durante tres estados de maduración de mora y agraz. El diseño estadístico que se siguió fue completamente aleatorio y los resultados muestran que las frutas en el último estado de madurez evaluado se diferencian por su perfil aromático, un contenido mayor de antocianinas (1,59 y 0,26 mg cyn-3-glu g-1 en agraz y mora, respectivamente) y fenoles totales (5,57 y 2,68 mg ácido caféico g-1 en agraz y mora, respectivamente). El comportamiento de las propiedades evaluadas es independiente en cada una de las frutas.
Downloads
References
Acosta-Montoya, Ó., F. Vaillant, S. Cozzano, C. Mertz, A. Pérez and M. Castro. Phenolic content and antioxidant capacity of tropical highland blackberry (Rubus adenotrichus Schltdl.) during three edible maturity stages. Food Chemistry 119(4): 1497-1501.
Asard, H., J.M. May and N. Smirnoff. 2004. Vitamin C, function and biochemistry in animals and plants. Garland Science, London, UK. 374 p.
Association of Analytical Communities A.O.A.C. 2005. Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: collaborative study. Journal of AOAC International 88(5):1269-1278.
Ávila, H., J. Cuspoca, G. Fischer, G. Ligarreto and M. Quicazan. 2007. Caracterización fisicoquímica y organoléptica del fruto de agraz (Vaccinium meridionale Swartz) Almacenado 1 a 2 ºC. Revista Facultad Nacional de Agronomía, Medellín 60(2): 4179-4193.
Balasundram, N., K. Sundramand and S. Samman. 2006. Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chemistry 99(1): 191-203.
Benedetti, S., S. Buratti, A. Spinardi, S. Mannino and I. Mignani. 2008. Electronic nose as a non-destructive tool to characterise peach cultivars and to monitor their ripening stage during shelf-life. Postharvest Biology and Technology 47(2): 181-188.
Bhattacharyya, N., S. Seth, B. Tudu, P. Tamuly, A. Jana, D. Ghosh, R. Bandyopadhyay, M. Bhuyan and S. Sabhapandit. 2007. Detection of optimum fermentation time for black tea manufacturing using electronic nose. Sensors and Actuators B: Chemical 122(2): 627-634.
Brezmes, J., E. Llobet, X. Vilanova, G. Saizand and X. Correig. 2000. Fruit ripeness monitoring using an electronic nose. Sensors and Actuators B: Chemical 69(3): 223-229.
Brown, J.E., V. Cheynier, M. Clifford, O. Dangles, K.M. Davies, C. Dufour, G.G. Duthie, D. Ferreira, T. Fossen, K.S. Gould, R.J. Grayer, K. Hostettmann, M. Jay, M. Jordheim, J.A. Kyle, C. Lister, J.P. Marais, A. Marston, K.E. Schwinn, D. Slade, K.M. Valant-Vetschera, N.C. Veitch, C.A. Williams, H. Wiseman and E. Wollenweber. 2006. Flavonoids: chemistry, biochemistry, and applications. Taylor and Francis Group, Boca Raton, USA. 1.212 p.
Castañeda, A., M.D. Pacheco, M.E. Páez, J.A. Rodríguez and C.A. Galán. 2009. Chemical studies of anthocyanins: A review. Food Chemistry 113(4): 859-871.
Castrejón, A.D., I. Eichholz, S. Rohn, L.W. Kroh and S. Huyskens-Keil. 2008. Phenolic profile and antioxidant activity of highbush blueberry (Vaccinium corymbosum L.) during fruit maturation and ripening. Food Chemistry 109(3): 564-572.
Chemler, J.A, K.M. Davies, V. de Freitas, S. Deroles, K.S. Gould, J.H. Hatier, M.A. Koffas, E. Leonard, S. Lev-Yadun, M.A. Lila, N. Mateus, T. Nakayama, S. Rasmussen, K. Saito, W.J. Steyn, M. Yamazaki and K. Yonekura-Sakakibara. 2009. Anthocyanins biosynthesis, functions, and applications. Springer, New York, USA. 345 p.
Chen, Q., H. Yu, H. Tang and X. Wang. 2012. Identification and expression analysis of genes involved in anthocyanin and proanthocyanidin biosynthesis in the fruit of blackberry. Scientia Horticulturae 141(0): 61-68.
Cocetta, G., K. Karppinen, M. Suokas, A. Hohtola, H. Häggman, A. Spinardi, I. Mignani and L. Jaakola.2012. Ascorbic acid metabolism during bilberry (Vaccinium myrtillus L.) fruit development. Journal of Plant Physiology 169(11): 1059-1065.
Contreras, J., L. Calderón, E. Guerra and B. García. Antioxidant capacity, phenolic content and vitamin C in pulp, peel and seed from 24 exotic fruits from Colombia. Food Research International 44(7): 2047-2053.
Dai, J., A. Gupte, L. Gates and R.J. Mumper. 2009. A comprehensive study of anthocyanin-containing extracts from selected blackberry cultivars: Extraction methods, stability, anticancer properties and mechanisms. Food and Chemical Toxicology 47(4): 837-847.
Diban G.N. 2008. Separación de aromas en etapas del procesado de zumos de frutas y bebidas. Tesis de doctorado en Ingeniería Química y Química Inorgánica: Facultad de Ingeniería. Universidad de Cantabria. 34 p.
Garzón, G.A., C.E. Narváez, K.M. Riedl and S.J. Schwartz. 2010. Chemical composition, anthocyanins, non-anthocyanin phenolics and antioxidant activity of wild bilberry (Vaccinium meridionale Swartz) from Colombia. Food Chemistry 122(4): 980-986.
Gorinstein, S., Z. Jastrzebski, H. Leontowicz, M. Leontowicz, J. Namiesnik, K. Najman, Y. S. Park, B. G. Heo, J. Y. Cho andJ. H. Bae. 2009. Comparative control of the bioactivity of some frequently consumed vegetables subjected to different processing conditions. Food Control 20(4): 407-413.
Instituto Colombiano de Normas Técnicas y Certificación (ICONTEC). 1999. NTC 4624. Jugos de frutas y hortalizas. Determinación del contenido de sólidos solubles. método refractométrico. Icontec, Bogotá [ Links ].
Instituto Colombiano de Normas Técnicas y Certificación (ICONTEC). 1999. NTC 440. Productos alimenticios. Métodos de ensayo. Icontec, Bogotá [ Links ].
Instituto Colombiano de Normas Técnicas y Certificación (ICONTEC). 1999. NTC 4106. Frutas frescas. Mora de castilla. Especificaciones. Icontec, Bogotá [ Links ].
Li, C., G.W. Krewer, P. Ji, H. Scherm and S.J. Kays. 2010. Gas sensor array for blueberry fruit disease detection and classification. Postharvest Biology and Technology 55(3): 144-149.
Ligarreto, G.A. 2011. Agraz (Vaccinium meridionale Swartz), algunas prácticas de cultivo y poscosecha. Bogotá, Colombia. Universidad Nacional de Colombia. Facultad de Agronomía. Ministerio de Agricultura y Desarrollo Rural, Bogotá D.C. 23 p.
Livani, F., M. Ghorbanli and A. Sateeyi. Changes in antioxidant activity and content of phenolic compounds during the ripening process of elm-leaved blackberry fruit. International Journal of Agronomy and Plant Production 4(1): 88-93.
Magalhães, L. M., M.A. Segundo, S. Reis and J.L. Lima. 2008. Methodological aspects about in vitro evaluation of antioxidant properties. Analytica Chimica Acta 613(1): 1-19.
Meret, M., P. Brat, C. Mertz, M. Lebrun and Z. Günata, 2011. Contribution to aroma potential of Andean blackberry (Rubus glaucus Benth). Food Research International 44(1): 54-60.
Mitcham, E., C. Crisosto and A. Kader. 1998. Bushberries: blackberry, blueberry, cranberry, raspberry: recommendations for maintaining postharvest quality. In: University of California, http://postharvest.ucdavis.edu/PFfruits/Bushberries/; accessed: May 2012.
Müller, L., S. Gnoyke, A.M. Popken and V. Böhm. 2010. Antioxidant capacity and related parameters of different fruit formulations. LWT - Food Science and Technology 43(6): 992-999.
Murcia, A.M., A.M. Jiménez and M. Martínez-Tomé. 2009. Vegetables antioxidant losses during industrial processing and refrigerated storage. Food Research International 42(8): 1046-1052.
Niki, E. 2010. Assessment of Antioxidant Capacity in vitro and in vivo. Free Radical Biology and Medicine 49(4): 503-515.
Oliveira, I., P. Baptista, R. Malheiro, S. Casal, A. Bento and J.A. Pereira. 2011. Influence of strawberry tree (Arbutus unedo L.) fruit ripening stage on chemical composition and antioxidant activity. Food Research International 44(5): 1401-1407.
Pathange, L.P., P. Mallikarjunan, R.P. Marini, S. O'Keefeand and D. Vaughan. 2006. Non-destructive evaluation of apple maturity using an electronic nose system. Journal of Food Engineering 77(4): 1018-1023.
Peris, M. and L. Escuder-Gilabert. 2009. A 21st century technique for food control: Electronic noses. Analytica Chimica Acta 638(1): 1-15.
Perkins-Veazie, P., J.R. Clark, D.J. Huber and E.A. Baldwin. 2000. Ripening physiology in 'Navaho' Thornless blackberries: color, respiration, ethylene production, softening, and compositional changes. Journal of the American Society for Horticultural Science 125(3): 357-363.
Pietta, P., M. Minoggio and L. Bramati. 2003. Plant polyphenols: structure, occurrence and bioactivity. pp: 257-312. In: Atta-Ur, R. (ed.). Studies in natural products chemistry. Elsevier, Amsterdam, The Netherlands. 491 p.
Shui, G. and L.P. Leong. 2002. Separation and determination of organic acids and phenolic compounds in fruit juices and drinks by high-performance liquid chromatography. Journal of Chromatography A 977(1): 89-96.
Torri, L., N. Sinelliand and S. Limbo. 2010. Shelf life evaluation of fresh-cut pineapple by using an electronic nose. Postharvest Biology and Technology 56(3): 239-245.
United States Department of Agriculture USDA. 2010. USDA National Nutrient Database for Standard Reference, Release 22 Nutrient Lists, http://www.ars.usda.gov/Services/docs.htm?docid=18877; accessed: September 2010.
Vasco, C., J. Rualesand and A. Kamal-Eldin. 2008. Total phenolic compounds and antioxidant capacities of major fruits from Ecuador. Food Chemistry 111(4): 816-823.
Vicente, A., G.A. Manganaris, G. Sozzi and C. Cristoso. 2009. Nutritional quality of fruits and vegetables. pp: 57-106. In: Florkowski W., R. Shewfelt, B. Brueckner and S. Prussia. (eds.). Postharvest handling: a systems approach. Second edition. Academic Press, Massachusetts, USA. 640 p.
Wang, L.S. and G.D. Stoner. 2008. Anthocyanins and their role in cancer prevention. Cancer Letters 269(2): 281-290.
Zuluaga, C.M., A.C. Diaz and M.C. Quicazán. 2011. Estandarización y validación del método de análisis del perfil aromático por nariz electrónica. Ingeniería e Investigación 31(2): 65-73.
License
Copyright (c) 2015 Revista Facultad Nacional de Agronomía Medellín

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The journal allows the author(s) to maintain the exploitation rights (copyright) of their articles without restrictions. The author(s) accept the distribution of their articles on the web and in paper support (25 copies per issue) under open access at local, regional, and international levels. The full paper will be included and disseminated through the Portal of Journals and Institutional Repository of the Universidad Nacional de Colombia, and in all the specialized databases that the journal considers pertinent for its indexation, to provide visibility and positioning to the article. All articles must comply with Colombian and international legislation, related to copyright.
Author Commitments
The author(s) undertake to assign the rights of printing and reprinting of the material published to the journal Revista Facultad Nacional de Agronomía Medellín. Any quotation of the articles published in the journal should be made given the respective credits to the journal and its content. In case content duplication of the journal or its partial or total publication in another language, there must be written permission of the Director.
Content Responsibility
The Faculty of Agricultural Sciences and the journal are not necessarily responsible or in solidarity with the concepts issued in the published articles, whose responsibility will be entirely the author or the authors.

