Bactericidal Evaluation of Lactic Acid Bacteria Isolated in Sow Colostrum Against Salmonella tiphymurium

Published

2015-01-01

Evaluación de la Actividad Bactericida de Bacterias Ácido Lácticas Aisladas en Calostro de Cerdas Frente a Salmonella typhimurium

Keywords:

Actividad antimicrobiana, calostro de cerdas, salud animal, ácidos orgánicos. (es)
Antimicrobial activity, sow colostrum, animal health, organic acids. (en)

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Authors

Juliana María Vélez Zea Universidad Nacional de Colombia – Sede Medellín – Facultad de Ciencias
Luz Adriana Gutiérrez Ramírez Corporación Universitaria Lasallista – Sede Medellín – Facultad de Ciencias Administrativas y Agropecuarias
Olga Inés Montoya Campuzano Universidad Nacional de Colombia – Sede Medellín – Facultad de Ciencias - Escuela de Biociencias

Abstract (es)
Abstract (en)

En los sistemas de crianza intensiva especialmente en cerdos, la resistencia antimicrobiana de algunas bacterias patógenas, se ha convertido en un problema mundial, tanto para los productores como para los consumidores. El uso de microorganismos probióticos aislados de calostro de cerda criadas en sistemas intensivos han demostrado eficacia en la inhibición del crecimiento de algunos patógenos entéricos. Se eligieron dos cepas denominadas como BAL1 y BAL3, a las cuales se les evaluó el potencial bactericida de los extractos bacterianos sobre el patógeno Salmonella thipymurium, por el método de difusión en pozos en agar Mueller Hinton. Los extractos se obtuvieron ajustando el inóculo a 0,5 McF en caldo MRS, centrifugados a 6.000 rpm por 5 minutos y filtrados con membrana de 0,2 µm. Ambos extractos mostraron tener un alto potencial bactericida frente a esta bacteria, con promedios de halo de inhibición de 11 mm in vitro.

In intensive farming systems especially in pigs, the antimicrobial resistance of some pathogenic bacteria has become a global problem, both for producers and consumers. The use of probiotic microorganisms isolated of sow colostrum in intensive farms have demonstrated the ability to inhibit the growth of some enteric pathogens. Two of these strains named as BAL1 and BAL3 were chosen for evaluate the bactericidal potential of their extracts against the pathogen Salmonella typhimurium. The well diffusion method, in Mueller Hinton agar was used. The BAL extracts were obtained, adjusted the BAL inoculum in MRS broth at 0.5 MacFarland, centrifuged at 6,000 rpm for 5 minutes and filtered with a 0.2 um membrane. Both extracts demonstrated to have bactericidal activity against this bacterium with mean inhibition halos of 11 mm in vitro.

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