Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation

Published

2016-07-01

Efecto de plasma seminal y espermatozoides de verracos valorados por congelabilidad sobre la criopreservación seminal

Keywords:

Boar, Reproduction, Seminal preservation, Seminal quality (en)
Cerdo, Reproducción, Preservación seminal, Calidad seminal (es)

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Authors

Francisco Javier Henao Uribe Universidad de Caldas
Julian Valencia Giraldo Universidad de Caldas
Henry Mesa Universidad de Caldas
Germán Gómez Londoño Universidad de Caldas

Abstract (en)
Abstract (es)

The aim of this study was to determine the effect of sperm and seminal plasma (SP) on the freezability of porcine semen. Semen of eight commercial males from two farms in the central-western region of Colombia (four boars in each farm) was frozen and tested to select two males with high freezability (MHF) and two with low freezability (MLF), according to the percentage of functionally competent sperm (FCS). Immediately after the collection was completed, the SP and sperm from the males selected were separated by centrifugation to combine the two types of plasma with the two types of sperm, incubate them for three hours and then freeze them. The variables evaluated were: sperm morphology, structural and functional integrity of plasmatic membrane, progressive and total motility, DNA fragmentation, acrosome integrity, capacitated sperm and FCS. The combination of sperm and plasma of MHF recorded the highest value (P<0.01) of acrosome integrity (24.3 ± 0.082 vs 6.076 ± 0.16) when compared to MLF plasma and cells. Membrane structural integrity was higher (P<0.01) with MHF (53.56 ± 0.0395) than with MLF plasma (47.49 ± 0.0419). The differences in porcine semen freezability depend on interactions between seminal plasma and sperm.

El objetivo del presente trabajo fue determinar el efecto de los espermatozoides y del plasma seminal (PS) sobre la congelabilidad del semen porcino. El semen de ocho machos comerciales, de dos granjas del centro-occidente de Colombia (cuatro machos en cada granja), fue congelado y evaluado para seleccionar dos machos con alta congelabilidad (MAC) y dos con baja (MBC), según el porcentaje de espermatozoides funcionalmente competentes (EFC). Inmediatamente finalizada la colección, fueron separados por centrifugación el PS y los espermatozoides de los machos seleccionados, para combinar los dos tipos de plasma con los dos tipos de espermatozoides, incubar tres horas y congelar. Las variables evaluadas fueron: morfología espermática, integridad estructural y funcional de la membrana plasmática, movilidad progresiva y total, fragmentación del ADN, integridad acrosómica, espermatozoides capacitados y EFC. La combinación de espermatozoides y plasma de MAC registró el valor más alto (P<0,01) de integridad acrosómica (24,3 ± 0,082 vs 6,076 ± 0,16) al compararlo con células y plasma de MBC. La integridad estructural de membrana fue mayor (P<0,01) con plasma de MAC (53,56 ± 0,0395) que con el de MBC (47,49 ± 0,0419). Las diferencias en la congelabilidad del semen de los porcinos dependen de las interacciones entre el plasma seminal y los espermatozoides.

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References

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