Published

2017-05-01

Association of TLR6 gene polymorphisms with zootechnical parameters in Holstein cattle in northern Antioquia

Asociación de polimorfismos del gen TLR6 con parámetros zootécnicos en ganado Holstein del norte de Antioquia

Keywords:

Alelo, Bovinos, Genotipo, Inmunidad, PCR, RFLP (en)
alelos, ganado, genotipo, inmunidad, PCR, RFLP (es)

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Specialized dairy systems are one of the most important lines in the agricultural sector in Colombia and the world. Currently there are strategies, both of environmental management and animal breeding, seeking to optimize the production. TLR6 gene, as many others related to immunity, is proving to be a useful candidate for programs of Marker Assisted Selection (MAS). Four hundred thirty two Holstein cows from 4 municipalities of northern Antioquia were genotyped using PCR-RFLP for C1859A and A1980G polymorphism of TLR6 gene. Allelic frequencies of 57.87% were found for C allele and 42.13% for A allele of C1859A, and 57.99% for A allele and 42.01% for G allele of A1980G. Genotypic frequencies of C1859A were 84.26% and 15.74% for CA and CC, respectively, and no AA genotype for this polymorphism was found; phenotypic frequencies of A1980G were 25.93%, 64.12% and 9.95% for AA, GA and GG, respectively. Mixed models for repeated measures with random effect of the animal were used to determine the association between genotypes and milk yield, percentages of protein and fat, services per conception, calving interval, and SCS. Both polymorphisms were only significant (P<0.01) on the SCS feature, being C allele of C1859A and A allele of A1980G the most favorable. TLR6 gene showed an effect on quality milk trait SCS, as expected for its immunological role in the response against bacteria, and can be postulated as molecular marker for selection against infectious diseases of importance in animal production, such as mastitis.

Los sistemas de lechería especializada son uno de los renglones más importantes en el sector agropecuario de Colombia y del mundo. Actualmente existen estrategias, tanto de manejo ambiental como de mejoramiento animal, que buscan optimizar la producción. El gen TLR6, como muchos relacionados con la inmunidad, está demostrando ser un candidato útil para los programas de selección asistida por marcadores moleculares (MAS). Mediante PCR-RFLP, se genotipificaron 432 vacas Holstein de 4 municipios del Norte de Antioquia para los polimorfismos C1859A y A1980G del gen TLR6. Se encontraron frecuencias alélicas de 57,87% para el alelo C y 42,13% para el alelo A de C1859A, y 57,99% para el alelo A y 42,01% para el alelo G de A1980G. Las frecuencias genotípicas de C1859A fueron 84,26% y 15,74% para CA y CC, respectivamente, y no se halló el genotipo AA para este polimorfismo; las frecuencias fenotípicas de A1980G fueron 25,93%, 64,12% y 9,95% para AA, GA y GG, respectivamente. Se utilizaron modelos mixtos de repeticiones con efecto aleatorio del animal para determinar asociación entre los genotipos y las características zootécnicas producción de leche, porcentajes de proteína y grasa, servicios por concepción, IEP y SCS. Ambos polimorfismos solo tuvieron efecto significativo (P<0,01) sobre la característica SCS, siendo el alelo C de C1859A y el alelo A de A 1980G los más favorables. El gen TLR6, mostró un efecto sobre el parámetro SCS de calidad de leche, como se espera por su role inmunológico en la respuesta contra bacterias, y puede ser postulado como marcador molecular para la selección en contra de enfermedades infecciosas de importancia en producción animal, como la mastitis.

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