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Inheritance of lethal gene ‘luteus-Pa’ from cacao progenies obtained by self and cross-fertilization
Herencia del gen letal ‘luteus-Pa’ en progenies de cacao obtenidas por autofecundación y fecundación cruzada
Keywords:
Cacao, Self-cross pollination, Lethal factor, Mendelian segregation, Chi-square (X2) test (en)Cacao, polinización artificial, gen letal, segregación mendeliana, prueba Chi-cuadrado (X2) (es)
With the aim to study the inheritance of lethal gene ‘luteus-Pa’ in three derivated progenies from self and cross-fertilization of cacao (Theobroma cacao L) varieties, a study was carried out during January to September 2013. As genetic material C-25, S-5 y Pa-150 varieties were utilized and this one were self and cross pollinated and their progenies were divided in two replicates with 100 seeds each and sowed in plastic recipients containing a substrate 2:1(soil: sand). In order to contrast the observed segregation with its expected homologous 3:1 (monogenic inheritance), the Chi-square (X2) test was utilized. The results shown that only one from self-fertilized progeny of C-25 variety exhibited the mendelian segregation 3:1 in both replicates, so that it lead to infer this one is carrier of lethal gene ‘luteus-Pa’ in heterozygote condition whereas that the progeny from Pa-150 x C-25 cross no found none lethal seedling and submitted to Chi-square (X2) test it could be to infer this one no segregate in the proportion 3:1 therefore, the Pa-150 variety is no carrier of this lethal gene. A better comprehension on photobiological origin, supported by molecular markers and the functional genomics of this mutant can help to identify and understand the factors involve in the photosynthetic mechanisms.
Con la finalidad de estudiar la herencia del gen letal ‘luteus-Pa’ en progenies derivadas de autofecundación y fecundación cruzada detres variedades de cacao (Theobroma cacao L), se realizó el ensayo entreabril a julio del 2013. Como material genético se utilizó las variedades C-25, S-5 y Pa-150 que fueron auto y alopolinizados y sus progenies fueron divididas en 2 repeticiones de 100 semillas c/u. y sembradas en contenedores plásticos conteniendo un sustrato 2:1 (suelo: arena). Para contrastar la segregación observada con la esperada 3:1 (herencia monogénica), se utilizó la prueba Chi-cuadrado (X2). Los resultados muestran que sólo la progenie autofecundada de la variedad C-25 exhibió la segregación mendeliana 3:1 en ambas repeticiones, de modo cual se puede inferir que esta variedad es portadora del gen letal ‘luteus-Pa’en condición hererocigota; mientras que en la progenie de la cruza Pa-150 x C-25 al no encontrarse ninguna plántula letal y sometida a la prueba Chi-cuadrado (X2), se infiere que falla en segregar en una proporción 3:1; por consiguiente, la variedad Pa-150 no es portadora de este gen letal. Una mejor comprensión sobre el origen fotobiológico apoyado por marcadores moleculares y la genómica funcional de este mutante, puede ayudar a identificar y comprender mejor los factores involucrados en el mecanismo fotosintético.
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References
Baker NR and Hardwick P. 1972. Biochemical and physiological aspects of leaf development in cocoa (Theobroma cacao L): I. Development of chlorophyll and photosynthetic activity. New Phytology 72: 1315-1324. doi: 10.1111/j.1469-8137.1973. tb02109.x
Bartley BGD. 1969. Selfing of self-incompatible trees. In: Annual Report on Cacao Research. Trinidad. pp. 22-23. Bartley BGD. 2005. The genetics of the diversity. In: The genetic diversity of cacao and its utilization. CABI Publishing, Wallingford. pp. 279-297.
Bartley BGD, Yamada MM, Castro GCT and Melo GRP. 1983. Genetics of Theobroma cacao: occurrence of lethal factor Luteus Pa in family Parinari. Theobroma 13: 275-278.
Diaz C, Colombani V, Loudet O, Belluomo P, Moreau L, Daniel-Vedele F, Morot-Gaudry JF and Masclaux-Daubresse C. 2006. Leaf yellowing and anthocyanin accumulation are two genetically independent strategies in response to nitrogen limitation in Arabidopsis thaliana. Plant Cell Physiol. 47(1): 74-83. doi: 10.1093/pcp/pci225
Jarvis P, Dormann P, Peto CA, Lutes J, Benning C and Chory J. 2000. Galactolipid deficiency and abnormal chloroplast development in the Arabidopsis MGD synthase 1 mutant. Proceedings of the National Academy of Sciences 97(14): 8175-8179. doi: 10.1073/pnas.100132197
Kosuge K, Watanabe N and Kuboyama T. 2011. Comparative genetic mapping of homologous genes for the Chlorina phenotype in the genus Triticum. Euphytica 179(2): 257-263. doi: 10.1007/s10681-010-0302-0
Meinke D, Muralla R, Sweeney S and Dickerman A. 2008. Identifying essential genes in Arabidopsis thaliana. Trends in Plant Science 13(9): 483-491. doi: 10.1016/j.tplants.2008.06.003
Migge A and Becker, T. 2000. Greenhouse-grown conditionally lethal tobacco plants obtained by expression of plastidic glutamine synthetase antisense RNA may contribute to biological safety. Plant Science 153(2): 107-112. doi: 10.1016/S0168-9452(99)00232-0
Ning Li, Jizeng Jia, Chuan Xia, Xu Liu and Xiuying Kong. 2013. Characterization and mapping of novel chlorophyll deficient mutant genes in durum wheat. Breeding Science 63(2): 169-175. doi: 10.1270/jsbbs.63.169
Reed S, Atkinson T, Gorecki C, Espinoza K, Przybylski S, Goggi A, Palmer R and Sandhu D. 2014. Candidate gene identification for a lethal chlorophyll-deficient mutant in soybean. Agronomy 4: 462-469. doi: 10.3390/agronomy4040462
Rehem BC, Almeida AAA, Figueiredo GSF, Gesteira AS, Santos SC, Corrêa1 RX, Yamada MM and Valle RR. 2016. Differential expression of the lethal gene Luteus-Pa in cacao of the Parinari series. Genetics and Molecular Research 15(1): 1-14. doi: 10.4238/gmr.15014925
Rehem, BC, Almeida AAA, Santos IC, Gómez FP. 2011. Photosynthesis, chloroplast ultrastructure, chemical composition and oxidative stress in Theobroma cacao L. hybrids with the lethal genes Luteus-Pa mutant. Photosynthetica 49(1): 127-139. doi: 10.1007/s11099-011-0021-3
Rehem BC, Almeida, AAA, Corrêa RX, Gesteira AS, Yamada MM and Valle RR, 2010. Genetic mapping of Theobroma cacao (Malvaceae) seedlings of the Parinari series, carriers of the lethal gene Luteus-Pa. Genetics and Molecular Research 9(3): 1775-1784. doi: 10.4238/vol9-3gmr889
Ryder E, Kim Z and Waycott W. 1999. Inheritance and epistasis studies of chlorophyll deficiency in lettuce. Journal of the American Society for Horticultural Science. 124(6): 636-640.
Yamada MM. 1982. Heranca do fator compatibilidade em Theobroma cacao L. I. Relacoes fenotípicas na familia Pa’ (Parinari). Revista Theobroma (Brasil) 12(3): 163-167.
Yamada MM, Carvalho R y Pires J. 2005. Identificacao de gene deletéreo “Luteus-Pa” em accessos de Theobroma cacao L. da serie Parinari. Nota científica. Agrotrópica 17: 81-82.
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