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Complete genome sequence of a Passion fruit yellow mosaic virus (PFYMV) isolate infecting purple passionfruit (Passiflora edulis f. edulis)
Secuenciación del genoma completo de un aislamiento del Passion fruit yellow mosaic virus (PFYMV) que infecta gulupa (Passiflora edulis f. edulis)
Keywords:
next-generation sequencing, Passifloraceae, Tymovirus, viral genomes (en)Secuenciación de nueva generación, Passifloraceae, Tymovirus, genomas virales (es)
Purple passion fruit (Passiflora edulis f. edulis), also known as gulupa, is a vine plant of the familiy Passifloraceae, which in recent years has gained importance in the world fruit market due to its exotic nature and excellent organoleptic properties. Although the demand for gulupa in Colombia has increased significantly to become one of the most important fruit exports, the cultivated area has been in decline since 2009 due to the impact of plant diseases. Cucumber mosaic virus (CMV), soybean mosaic virus (SMV) and cowpea aphid borne mosaic virus (CABMV) are amongst the main viruses found infecting gulupa in Colombia. To further characterize the virome of gulupa, a deep sequencing transcriptome study was performed from a producing region in eastern Antioquia. Based on the results of next-generation sequencing (NGS), we report the genome sequence of a tymovirus infecting this plant. Phylogenetic analysis revealed this virus to be a close relative of Passion fruit yellow mosaic virus (PFYMV), Cassia yellow mosaic-associated virus (CYMaV) and Calopogonium yellow vein virus (CYVV). To date, only a 1115 nt segment comprising the RdRP-CP region of PFYMV has been reported; this sequence shares 84.79% and 95.24% identities at the nucleotide and amino acid levels with the purple passionfruit tymovirus suggesting that the detected virus is a PFYMV isolate (PFYMV_Antioquia). Finally, RT-qPCR and Sanger sequencing using specific primers confirmed the presence of PFYMV in different purple passionfruit crops in Antioquia. This is the first complete genome sequence of a PFYMV isolate reported in the world.
El maracuyá morado (Passiflora edulis f. edulis), también conocido como gulupa, es una planta enredadera de la familia Passifloraceae, que recientemente ha tomado importancia en el mercado mundial de frutas exóticas, gracias a sus excelentes características organolépticas. Aunque la demanda de gulupa en Colombia se ha incrementado significativamente en los últimos años al ser una de las frutas que más exporta el país, su área cultivada ha venido reduciéndose desde 2009, debido al impacto negativo de diferentes enfermedades. Hasta el momento se conocía que el cucumber mosaic virus (CMV), soybean mosaic virus (SMV) y cowpea aphid borne mosaic virus (CABMV) eran los principales virus que afectan este cultivo en Colombia. Con el fin de continuar caracterizando el viroma de la gulupa, en el presente estudio se realizó una secuenciación del transcriptoma de plantas cultivadas en el oriente de Antioquia. Con base en los resultados obtenidos para la secuenciación de nueva generación (NGS), se detectó un tymovirus infectando estas plantas. Los análisis filogenéticos indicaron que dicho virus está relacionado con las especies: passion fruit yellow mosaic virus (PFYMV), Cassia yellow mosaic-associated virus (CYMaV) y Calopogonium yellow vein virus (CYVV). Ya que la secuencia aquí reportada compartió niveles de identidad de 84,79% y 95,24% en nucleótidos y aminoácidos, con una secuencia de 1115 nt del segmento que comprende la region RdRP-CP del PFYMV, el virus que infecta gulupa corresponde a un aislamiento (PFYMV_Antioquia) de esta especie. Finalmente, mediante reacciones de RT-qPCR y secuenciación Sanger utilizando cebadores específicos se confirmó la presencia del PFYMV en diferentes cultivos de gulupa de Antioquia. Este es el primer reporte de un genoma completo del PFYMV en el mundo.
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