Effect of drying methods on physical and chemical properties of Ilex guayusa leaves
Efecto de métodos de secado sobre las propiedades físicas y químicas de las hojas de Ilex guayusa
Keywords:
Ilex guayusa, Caffeine, Air drying, Conventional oven, Solar drying (en)Ilex guayusa, Cafeína, Secado al aire, Horno convencional, Secado solar (es)
The influence of air-drying (AD), convection oven (CO) and solar drying (SOD) on the physical and chemical properties of Ilex guayusa leaves is discussed. Total ash (%), acid-insoluble ash (%), watersoluble ash (%), residual moisture (%) and caffeine content (%) were estimated. Additionally, alkaloids,
flavonoids, reducing sugars, saponins, steroids, quinones, fats, phenols and tannins detection of dry leaves extracts were assessed. The results revealed that parameters of physical analysis were not affected by the drying techniques. The highest amount of secondary metabolites in ethanol and
aqueous extracts were detected. In general, convection oven dried leaves showed the highest caffeine content (3.71%) and the lowest drying time (13 h) compared to other drying methods. The results revealed a fast method to dry Ilex guayusa leaves and indicate that this species possess a variety of bioactive compounds beneficial for health. Our results revealed an effective quick method to dry Ilex guayusa leaves without altering the content of beneficial bioactive components.
En este trabajo se discute la influencia del secado al aire (AD), el horno de convección (CO) y el secado solar (SOD) sobre las propiedades físicas y químicas de las hojas de Ilex guayusa. Se
estimaron las cenizas totales (%), las cenizas insolubles en ácido (%), las cenizas solubles en agua (%), la humedad residual (%) y el contenido de cafeína (%). Además, se analizó la presencia los alcaloides, flavonoides, azúcares reductores, saponinas, esteroides, quinonas, grasas, fenoles y taninos en extractos de hojas secas. Los resultados revelaron que los parámetros del análisis físico no se vieron afectados por las técnicas de secado. La mayor cantidad de metabolitos secundarios se detectó en el extracto etanólico y acuoso. En general, las hojas secadas al horno de convección mostraron el mayor contenido de cafeína (3,71%) y el menor tiempo de secado (13 h) en comparación con los otros métodos de secado empleados. Los resultados obtenidos revelaron un método rápido para secar hojas de Ilex guayusa sin alterar el contenido de componentes bioactivos beneficiosos para la salud, estudios que no han sido reportados.
Downloads
References
AOAC. 2005. Official Method of Analysis. 18th edition. Washington DC.
Ambasta S, Ramachandran K, Kashyapa K and Chand R. 1986. The useful plants of India. Publication and Information Directorate, CSIR, New Delhi. 109 p.
Cai Y, Luo Q, Sun M and Corke H. 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sciences 74: 2157–2184.
Christensen CM and Kaufmann HH. 1974. Microflora. pp. 158-192. In: Christensen CM (ed.). Storage of cereal grains and their products. American Association of Cereal Chemists, St Paul, Minnesota.
Díaz-Maroto M, Pérez-Coello M, González Viñas M and Cabezudo M. 2003. Influence of drying on the flavor quality of spearmint (Mentha spicata L.). Journal of Agricultural and Food Chemistry 51(5): 1265–1269. doi: 10.1021/JF020805L
Dueñas J, Jarrett C, Cummins I and Logan–Hines E. 2016. Amazonian Guayusa (Ilex guayusa Loes.): A historical and ethnobotanical overview. Economic Botany 70(1): 85–91. doi: 10.1007/s12231-016-9334-2
Figiel A and Michalska A. 2016. Overall quality of fruits and vegetables products affected by the drying processes with the assistance of Vacuum-Microwaves. International Journal of Molecular Sciences 18(1): 71. doi: 10.3390/ijms18010071
García F. 2014. Evaluación de los efectos del proceso de secado sobre la calidad de la Stevia (Stevia rebaudiana Bertoni) y la Hierbabuena (Mentha spicata). Tesis Magister en Ingeniería Agrícola. Facultad de Ingeniería. Universidad Nacional de Colombia, Bogotá. 126 p.
Isolabella S, Cogoi L, López P, Anesini C, Ferraro G and Filip R. 2010. Study of the bioactive compounds variation during Yerba Mate (Ilex paraguariensis) processing. Food Chemistry 122 (3): 695–99. doi: 10.1016/J.FOODCHEM.2010.03.039.
Kapp R, Mendes O, Shambhu R, McQuate R and Kraska R. 2016. General and genetic toxicology of Guayusa concentrate (Ilex guayusa). International Journal of Toxicology 35(2): 222–42. doi: 10.1177/1091581815625594.
Kim S, Hun-Gu S, Sang Ho C, Sangryeol R and Dong-Hyun K. 2012. Radio-frequency heating to inactivate Salmonella typhimurium and Escherichia coli O157:H7 on black and red pepper spice. International Journal of Food Microbiology 153(1–2): 171–75. doi: 10.1016/j.ijfoodmicro.2011.11.004.
Kothiyal S, Sati S, Rawat M, Sati M, Semwal D, Semwal R, Sharma A, Rawat B and Kumar A. 2012. Chemical constituents and biological significance of the genus Ilex (Aquifoliaceae). Natural Products Journal 2: 212-224.
Lima VLAG, Melo EA and Lima DES. 2004. Nota Prévia: Teor de compostos fenólicos totais em chás brasileiros. Brazilian Journal of Food Technology 7(2): 187–190.
Metro D, Cernaro V, Santoro D, Papa M, Buemi M, Benvenga S and Manasseri L. 2017. Beneficial effects of oral pure caffeine on oxidative stress. Journal of Clinical and Transnational Endocrinology 10: 22–27. doi: 10.1016/J.JCTE.2017.10.001.
Miranda M and Cuellar A. 2001. Manual de prácticas de laboratorio. Farmacognosia y productos naturales. Editorial Félix Varela, La Habana. pp. 25-49, 74-79.
Nishitani E and Yuko M. 2004. Simultaneous determination of catechins, caffeine and other phenolic compounds in tea using new HPLC method. Journal of Food Composition and Analysis 17(5): 675–85. doi: 10.1016/j.jfca.2003.09.009.
Overing J and Passes A. 2000. The anthropology of love and anger: The aesthetics of conviviality in native Amazonia. New York. Routledge xiv, 305: 24
Pacha JAG. 2012. Comprobacion del efecto adelgazante de la tintura de Guayusa (Ilex guayusa) en ratones (Mus musculus) con sobrepeso inducido. Tesis Bioquímico Farmacéutico. Facultad de Ciencias. Escuela de Bioquímica y Farmacia. Escuela Superior Politécnica de Chimborazo, Riobamba, Ecuador. 112 p.
Pardau MD, Pereira ASP, Apostolides Z, Serem JC and Bester MJ. 2017. Antioxidant and anti-inflammatory properties of Ilex guayusa tea preparations: a comparison to Camellia sinensis teas. Food and Function 8(12): 4601-4610. doi: 10.1039/c7fo01067b.
Radice M, Scalvenzi L and Sablón N. 2017. Ilex Guayusa: A Systematic Review of its traditional uses, chemical constituents, biological activities and biotrade opportunities. In: Proceedings of MOL2NET 2016, International Conference on Multidisciplinary Sciences, Second edition, 3868. MDPI, Basel, Switzerland: doi: 10.3390/mol2net-02-03868.
Reis M, Mariot A and Steenbock W. 2003. Diversidade e domesticação de plantas medicinais. In: Simões CMO, Schenkel EP, Gosman G, Mello JCP, Mentz LA, Petrovick PR. (Eds.). Farmacognosia: da planta ao medicamento. Fifth edition. Editora da UFRGS/Editora da UFSC, Porto Alegre/Florianópolis. pp. 45-74. 3–74.
Rojo De Camargo M and Toledo M. 1999. HPLC determination of caffeine in tea, chocolate products and carbonated beverages. Journal of the Science of Food and Agriculture 79 (13): 1861–1864. doi: 10.1002/(SICI)1097-0010(199910)79:13<1861::AID-JSFA448>3.0.CO;2-Y
Tene V, Malagón O, Vita FP, Vidari G, Armijos C and Zaragoza T. 2007. An ethnobotanical survey of medicinal plants used in Loja and Zamora-Chinchipe, Ecuador. Journal of Ethnopharmacology 111(1): 63–81. doi: 10.1016/j.jep.2006.10.032.
Turnbull D, Rodricks J, Gregory F and Chowdhury F. 2017. Caffeine and cardiovascular health. Regulatory Toxicology and Pharmacology 89: 165–85. doi: 10.1016/J.YRTPH.2017.07.025.
Venditti E, Bacchetti T, Tiano L, Carloni P, Greci L and Damiani E. 2010. Hot vs. cold water steeping of different teas: Do they affect antioxidant activity? Food Chemistry 119: 1597–1604.
Verpoorte R, Alfermann A and Johnson T (Eds.). 2007. Applications of plant metabolic engineering. Springer, Netherlands. doi: 10.1007/978-1-4020-6031-1
Villacís-Chiriboga J, García-Ruiz A, Baenas N, Moreno D, Meléndez-Martínez A, Stinco C, Jerves-Andrade L, León-Tamariz F, Ortiz-Ulloa J and Ruales J. 2017. Changes in phytochemical composition, bioactivity and in vitro digestibility of guayusa leaves (Ilex guayusa Loes.) in different ripening stages. Journal of the Science of Food and Agriculture 98(5): 1927-1934. doi: 10.1002/jsfa.8675
WHO. World Health Organization. 1998. Quality control methods for medicinal plant materials. WHO/PHARM/92.559, Ginebra.
WHO. World Health Organization. 2011. Quality control methods for herbal materials. World Health Organization, Geneva. http://www.who.int/iris/handle/10665/44479
Zhi-cen L. 1980. General control methods for vegetable drugs. Comparative study of methods included in thirteen pharmacopoeias a proposal on their international unification. WHO/PHARM/80.50:8-39, Ginebra.
License
Copyright (c) 2018 Patricia Isabel Manzano Santana

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The journal allows the author(s) to maintain the exploitation rights (copyright) of their articles without restrictions. The author(s) accept the distribution of their articles on the web and in paper support (25 copies per issue) under open access at local, regional, and international levels. The full paper will be included and disseminated through the Portal of Journals and Institutional Repository of the Universidad Nacional de Colombia, and in all the specialized databases that the journal considers pertinent for its indexation, to provide visibility and positioning to the article. All articles must comply with Colombian and international legislation, related to copyright.
Author Commitments
The author(s) undertake to assign the rights of printing and reprinting of the material published to the journal Revista Facultad Nacional de Agronomía Medellín. Any quotation of the articles published in the journal should be made given the respective credits to the journal and its content. In case content duplication of the journal or its partial or total publication in another language, there must be written permission of the Director.
Content Responsibility
The Faculty of Agricultural Sciences and the journal are not necessarily responsible or in solidarity with the concepts issued in the published articles, whose responsibility will be entirely the author or the authors.

