Improvement of the agricultural productivity of lettuce and radish by using efficient microorganisms
Mejoramiento de la productividad agrícola de la lechuga y el rábano con el uso de microorganismos eficientes
Keywords:
biofertilizer, Lactuca sativa, organoponic garden, Raphanus sativus, (en)biofertilizante, Lactuca sativa, jardín organopónico, Raphanus sativus (es)
The aim of this work was to evaluate the effect of the application of efficient microorganisms (EM) on the productive performance of lettuce and radish. The experiment was carried out in an organoponic culture in the municipality of Matanzas, Cuba, from December 2017 to January 2018. Five treatments were studied: control (0), EM 8 mL m-2 at 0 days after the transplant (DAT) (EM 8-0), EM 8 mL m-2 at 15 DAT (EM 8-15), EM 10 mL m-2 at 0 DAT (EM 10-0), and EM 10 mL m-2 at 15 DAT (EM 10-15). A randomized complete block design was set with four replications per treatment. An ANOVA was applied to perform the statistical data analysis, and the Duncan’s Multiple Range Test (P<0.05) was used for the comparison of means. The statistic program used was STATISTICA, version 6.0 over Windows. The parameters evaluated in lettuce were the total number of leaves, number of commercial leaves, the diameter of leaf rosette, and yield. The evaluated parameters for radish were fleshy root’s diameter and weight, and yield. The results indicated a positive effect on growth-response with the application of the bio-product, which can be considered a promissory alternative for vegetable production in organoponic garden conditions. The application of EM 10 mL m-2 at 0 and 15 DAT showed the best productive behavior for both crops.
El objetivo de este trabajo fue evaluar el efecto de la aplicación de microorganismos eficientes (EM) en el rendimiento productivo de cultivos de lechuga y rábano. El experimento se llevó a cabo en un cultivo organopónico en el municipio de Matanzas, Cuba, desde diciembre de 2017 hasta enero de 2018. Se estudiaron cinco tratamientos: control (0), EM 8 mL m-2 a los 0 días después del trasplante (DAT) (EM 8-0), EM 8 mL m-2 a 15 DAT (EM 8-15), EM 10 mL m-2 a 0 DAT (EM 10-0) y EM 10 mL m-2 a 15 DAT (EM 10-15). Se estableció un diseño de bloques completos al azar con cuatro repeticiones por tratamiento. Se aplicó un ANOVA para realizar el análisis estadístico de datos, y se utilizó la prueba de rango múltiple de Duncan (P<0.05) para comparar las medias. El programa estadístico utilizado fue STATISTICA, versión 6.0 en Windows. Los parámetros evaluados en lechuga fueron el número total de hojas, el número de hojas comerciales, el diámetro de la roseta y el rendimiento. Los parámetros evaluados para el rábano fueron el diámetro y peso de la raíz carnosa y el rendimiento. Los resultados indicaron un efecto positivo en la respuesta de crecimiento con la aplicación del bio-producto, que puede considerarse una alternativa promisoria para la producción de vegetales en condiciones organopónicas de jardín. La aplicación de EM 10 mL m-2 a 0 y 15 DAT mostró el mejor comportamiento productivo para ambos cultivos.
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References
Arismendi E. 2010. Microorganismos Eficientes, ¿fórmula mágica?. En: http://www.rapaluruguay.org/organicos/articulos/microorganismos_eficientes.html consulta: marzo 2018.
Basu S, Rabara R and Negi S. 2017. Towards a better greener future - An alternative strategy using biofertilizers. I: Plant growth promoting bacteria. Plant Gene. 12: 43-49. doi: 10.1016/j.plgene.2017.07.004
Berger B, Wiesner M, Brock AK, Schreiner M and Ruppel S. 2015. K. radicincitans, a beneficial bacteria that promotes radish growth under field conditions. Agronomy for Sustainable Development 35(4):1521–1528. doi: 10.1007/s13593-015-0324-z
Campo-Martínez ADP, Acosta RL, Morales S y Alfonso F. 2014. Evaluación de microorganismos de montaña (MM) en la producción de acelga en la meseta de Popayán. Biotecnología en el Sector Agropecuario y Agroindustrial 12(1): 79-87.
Changas-Junior AF, de Oliveira AG, de Oliveira LA, dos Santos GR, Changas LFB, Lopes da Silva AL and da Luz Costa J. 2015. Production of indole-3-acetic acid by Bacillus isolated from different soils. Bulgarian Journal of Agricultural Science. 21(2): 282–287.
Damam M, Kaloori K, Gaddam B and Kausar R. 2016. Plant growth promoting substances (Phytohormones) produced by rhizobacterial strains isolated from the rhizosphere of medicinal plants. International Journal of Pharmaceutical Sciences Review and Research 37(1): 130-136.
Duncan DB. 1955. Multiple range and multiple F test. Biometrics. 11(1): 1-42.
Estrada W, Chávez L, Jerez E, Nápoles MC, Sosa A, Cordoví C y Celeiro F. 2017. Efecto del Azofert® en el rendimiento de variedades de frijol común (Phaseolus vulgaris L.) en condiciones de déficit hídrico. Centro Agrícola 44(3): 36-42.
Ghaffari H, Gholizadeh A, Biabani A, Fallah A and Mohammadian M. 2018. Plant Growth Promoting Rhizobacteria (PGPR) Application with Different Nitrogen Fertilizer Levels in Rice (Oryza sativa L.). Pertanika Journal of Tropical Agricultural Science. 41(2): 715 – 728.
Ge C, Radnezhad H, Abari MF, Sadeghi M and Kashi G. 2016. Effects of biofertilizers and plant growth promoting bacteria on the growth characteristics of Asparagus officinalis. Applied Ecology and Environmental Research. 14(3): 547-558. doi: 10.15666/aeer/1403_547558
Grosu AI, Sicuia OA, Dobre A, Voaides C and Cornea C. 2015. Evaluation of some Bacillus spp. Strains for the biocontrol of Fusarium graminearum and F. culmorum in wheat. Agriculture and Agricultural Science Procedia. 6: 559-566. doi: 10.1016/j.aaspro.2015.08.085
GNAUS - Grupo Nacional de Agricultura Urbana. 2007. Manual Técnico para Organopónicos y Huertos Intensivos. Ciudad de La Habana, Cuba. 161 p.
GNAUS - Grupo Nacional de Agricultura Urbana, Suburbana y Familiar. 2015. Lineamientos de la Agricultura Urbana, Suburbana y Familiar para el año 2016. Ministerio de la Agricultura. INIFAT. La Habana, Cuba. p 14.
Hauka FIA, Moslam TM, Ghanem KhM and El-Shahat MM. 2016. Impact of some plant growth promoting rhizobacteria "pgpr" on organically cultivated spinach plants (Spinacia oleracea L.). Journal of Agricultural Chemistry and Biotechnology 7(9): 235 – 240.
IICA - Instituto Interamericano de Cooperación para la Agricultura. 2013. Tecnología de bajo costo: guía de manejo de microorganismos eficientes (ME). Proyecto Red SICTA, Cooperación Suiza en América Central. Managua, Nicaragua. p. 5-6.
Katiyar D, Hemantaranjan A and Singh B. 2016. Plant growth promoting Rhizobacteria-an efficient tool for agriculture promotion. Advances in Plants & Agriculture Research 4(6): 426‒434. doi:10.15406/apar.2016.04.00163
Khatab OH, Nasib MAA, Ghoneimy EA, Abo-Elnasr AA, Hassan HA-A, Hassan MYA and Attitalla IH. 2015. Role of Microorganisms in our life's as ecofrindely and replacement for chemical methods. International Journal of Pharmacy and Life Sciences 6(2):4221-4229.
Kumar S, Kumar S, Maji S and Pandey VK. 2016. Effect of inorganic fertilizers and bio-fertilizers on growth, yield and quality of radish (Raphanus sativus L.). International Journal of Plant Sciences (Muzaffarnagar) 11(1): 71-74. doi: 10.15740/HAS/IJPS/11.1/71-74
Mali DL, Singh V, Sarolia DK, Teli SK, Chittora A and Dhakar R. 2018. Effect of organic manures and bio-fertilizers on growth and yield of radish (Raphanus sativus L.) cv. Japanese white. International Journal of Chemical Studies 6(2): 1095-1098.
Mani AP and Anburani A. 2018. Organic nutrient management technique for enhancing growth and physiological parameters in radish (Raphanus sativus L.). Journal of Phytology 10(1): 40-42. doi: 10.25081/jp.2018.v10.3461
Menendez E and Garcia-Fraile P. 2017. Plant probiotic bacteria: solutions to feed the world. AIMS Microbiology 3(3): 502-524. doi: 10.3934/microbiol.2017.4.747
Mesa JR. 2016. Efecto de un biopreparado de producción local a base de microorganismos eficientes sobre diferentes cultivos en la provincia de Cienfuegos. En: Memorias IV Convención Internacional de Agrodesarrollo. Cuba.
Navia-Cuetia CA, Zemanate-Cordoba Y, Morales-Velasco S, Prado FA y Albán-López N. 2013. Evaluación de diferentes formulaciones de compostaje a partir de residuos de cosecha de tomate (Solanum lycopersicum L.). Biotecnología en el Sector Agropecuario y Agroindustrial 11(spe): 165 – 173.
Nghia NK, Tien TTM, Oanh NTK and Nuong NHK. 2017. Isolation and Characterization of Indole Acetic Acid Producing Halophilic Bacteria from Salt Affected Soil of Rice–Shrimp Farming System in the Mekong Delta, Vietnam. Agriculture, Forestry and Fisheries 6(3): 69-77. doi: 10.11648/j.aff.20170603.11
Núñez DB, Liriano R, Pérez Y, Placeres I y Sianeh Zawolo G. 2017. Respuesta de Daucus carota, L. a la aplicación de microorganismos nativos en condiciones de organopónico. Centro Agrícola 44(2): 29-35.
Pathak M, Tripathy P, Dash SK, Sahu GS and Pattanayak SK. 2017. Effect of source of nutrient on growth, yield and quality of Radish (Raphanus sativus L.) in radish - coriander cropping sequence. The Pharma Innovation Journal 6(12): 496-499.
Pedarza RO, Teixeira KRS, Fernández A, García de Salamone IB, Azcón R, Vera LDB y Bonilla R. 2010. Microorganismos que mejoran el crecimiento de las plantas y la calidad de los suelos. Ciencia y Tecnología Agropecuaria 11(2): 155-164. doi: 10.21930/rcta.vol11_num2_art:206
Pomboza-Tamaquiza P, León-Gordón OA, Villacís-Aldaz LA, Vega J y Aldáz-Jarrín JC. 2016. Influencia del biol en el rendimiento del cultivo de Lactuca sativa L. variedad Iceberg. Journal of the Selva Andina Biosphere 4(2): 84-92.
Ramírez-Gil JG. 2019. Dependency, colonization, and growth in Gmelina arborea inoculated with five strains of Arbuscular Mycorrhizal Fungi. Revista Facultad Nacional de Agronomía Medellín 72(2): 8775-8783. doi: 10.15446/rfnam.v72n2.74691
Robledo-Buriticá J, Aristizábal-Loaiza JC, Ceballos-Aguirre N and Cabra- Cendales T. 2018. Influence of plant growth-promoting rhizobacteria (PGPR) on blackberry (Rubus glaucus Benth. cv. thornless) growth under semi-cover and field conditions. Acta Agronómica 67(2): 258-263. doi: 10.15446/acag.v67n2.62572
Sabaté DC, Pérez C, Petroselli G, Erra-Balsells R and Audisio MC. 2018. Biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary on common bean by native lipopeptide-producer Bacillus strains. Microbiological Research 211: 21–30. doi: 10.1016/j.micres.2018.04.003.
Stanier R. 1996. Microbiología. Segunda Edición. Editorial Reverté S.A., Barcelona. 760 p.
Stamenković S, Beškoski V, Karabegović I, Lazić M and Nikolić N. 2018. Microbial fertilizers: A comprehensive review of current findings and future perspectives. Spanish Journal of Agricultural Research 16(1): 1-18. doi.org/10.5424/sjar/2018161-12117. doi: 10.5424/sjar/2018161-12117
Szczech M, Szafirowska A, Kowalczyk W, Szwejda-Grzybowska J, Włodarek A and Maciorowski R. 2016. The effect of plant growth promoting bacteria on transplants growth and lettuce yield in organic production. Journal of Horticultural Research 24(2): 101-107. doi: 10.1515/johr-2016-0026
Thakur D, Kaur M and Mishra A. 2017. Isolation and screening of plant growth promoting Bacillus spp. and Pseudomonas spp. and their effect on growth, rhizospheric population and phosphorous concentration of Aloe vera. Journal of Medicinal Plants Studies 5(1): 187-192. doi: 10.1007/s12088-017-0642-8.
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