Comparison of antioxidant contents of green and red leaf lettuce cultured in hydroponic systems under greenhouse, and conventional soil culture.
Comparación del contenido antioxidante de lechuga roja y verde cultivada en Sistema tradicional y cultivo hidropónico bajo invernadero
Keywords:
Antioxidants, Anthocyanins, Hydroponics, Aeroponics (en)Antioxidantes, Antocianinas, Sistema hidropónico, Sistema aeropónico (es)
Hydroponic technologies have increased the cultivated area under greenhouses covered with UV filter polyethylene film. However, there is a concern about the antioxidant contents of lettuces produced under these systems. In this study, it was compared to the antioxidant contents of green and red leaf lettuces produced in two hydroponic systems in a greenhouse, against the ones produced with the conventional cultivation system by Colombian farmers. The antioxidant analysis revealed significant statistical differences for DPPH and Anthocyanin analyses (P=0) versus all other culture systems. The best results were obtained in the conventional cultivation. Red lettuce obtained the highest value of FRAP (655.3±82.6 mgAAE 100g-1), ABTS (17.8±6.9 mmolTE 100g-1), total phenols (680.2±69.3 mgGAE 100g-1) and anthocyanin (126.2±6.9 mgC3G 100g-1). Green lettuce exhibited the most antioxidant activity of DPPH (20.7±5.6 mmolTE 100g-1). These results suggest a detrimental effect of the greenhouse covered with UV filter polyethylene film in the antioxidant production of lettuce. Finally, red leaf lettuce showed better antioxidant capacity independent of the cultivation system.
Las tecnologías hidropónicas han incrementado el área cultivada bajo invernadero cubierto con películas de polietileno con filtro UV. Sin embargo, hay una preocupación acerca del contenido de antioxidantes de las lechugas producidas en estos sistemas. En este estudio se compararon los contenidos antioxidantes de las lechugas de hoja verde y roja producidas en dos sistemas hidropónicos bajo invernadero contra las producidas en el Sistema de cultivo convencional de los agricultores colombianos. El análisis antioxidante reveló diferencias estadísticamente significativas para las técnicas DPPH y antocianinas entre los tres sistemas de cultivo. Los mejores resultados fueron obtenidos en el cultivo convencional. La lechuga roja obtuvo el valor más alto en FRAP (655.3±82.6 mgEAA 100g-1), ABTS (17.8±6.9 mmolET 100g-1), fenoles totales (680.2±69.3 mg EAG 100g-1) y antocianinas (126.2±6.9 mgE3GC 100g-1). Las lechugas verdes mostraron la mayor actividad antioxidante para DPPH (20.7±5.6 mmolET 100g-1). Estos resultados sugieren un efecto nocivo de los invernaderos cubiertos con películas de polietileno con filtro UV en la producción de antioxidantes en lechugas. Finalmente, la lechuga de hoja roja mostró mayor capacidad antioxidante independiente del tipo de cultivo.
References
Adesso S, Pepe G, Sommella E, Manfra M, Scopa A, Sofo A, Tenore GC, Russo M, Di Gaudio F, Autore G, Campiglia P and Marzocco S. 2016. Anti-inflammatory and antioxidant activity of polyphenolic extracts from Lactuca sativa (var. Maravilla de Verano) under different farming methods. Journal of the Science of Food and Agriculture 96(12): 4194-4206. doi: 10.1002/jsfa.7622
Alsadon AA, Al-Helal IM, Ibrahim AA, Abdel-Ghany AM, Al-Zaharani SM and Gulrez KH. 2016. Growth response of cucumber under greenhouses covered with plastic films. The Journal of Animal & Plant Sciences 26(1):139-148.
Barbosa GL, Gadelha FDA, Kublik N, Proctor A, Reichelm L, Weissinger E, Wohlleb GM and Halden RU. 2015. Comparison of Land, Water, and Energy Requirements of Lettuce Grown Using Hydroponic vs. Conventional Agricultural Methods. International Journal of Environmental Research and Public Health 12(6): 6879-6891. doi: 10.3390/ijerph120606879
Benzie IF and Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP Assay. Analytical Biochemistry 239(1): 70-76. doi: 10.1006/abio.1996.0292
Brand-Williams W, Cuvelier ME and Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT - Food Science Technology 28(1): 25-30. doi: 10.1016/S0023-6438(95)80008-5
Bunning M and Kendall P. 2012. Health Benefits and Safe Handling of Salad Greens. Fact Sheet No. 9.373. In: Colorado State University Extension, https://extension.colostate.edu/topic-areas/nutrition-food-safety-health/health-benefits-and-safe-handling-of-salad-greens-9-373/ accessed: November 2019.
Cemek B, Demir Y, Uzun S and Ceyhan V. 2006. The effects of different greenhouse covering materials on energy requirement, growth and yield of aubergine. Energy 31(12): 1780–1788. doi: 10.1016/j.energy.2005.08.004
Chen Y, Zhou B, Li J, Tang H, Tang J and Yang Z. 2018. Formation and Change of Chloroplast-Located Plant Metabolites in Response to Light Conditions. International Journal of Molecular Sciences 19(3): 654. doi: 10.3390/ijms19030654
Chon SU, Boo HO, Heo BG and Gorinstein S. 2012. Anthocyanin content and the activities of polyphenol oxidase, peroxidase and phenylalanine ammonialyase in lettuce cultivars. International Journal Food Science Nutrition 63(1): 45-8. doi: 10.3109/09637486.2011.595704
Cooper A. 1988. The ABC of NFT. Nutrient Film Technique: The World's First Method of Crop Production Without a Solid Rooting Medium. Intl Specialized Book Services, London. pp. 3-123.
Dissanayaka A, Rodriguez TO, Di S, Yan F, Githiri SM, Rojas Rodas F, Abe J and Takahashi R. 2016. Quantitative trait locus mapping of soybean maturity gene E5. Breeding Science 66(3): 407–415. doi: 10.1270/jsbbs.15160
FAO. 2013. The state of food and agriculture. Food Systems For Better Nutrition. In: FAO, http://www.fao.org/3/i3300e/i3300e00.htm accessed: November 2019.
FAO. 2017. Crops. In: FAOSTAT, http://www.fao.org/faostat/en/#data/QC/visualize accessed: March 2018.
Gan YZ and Azrina A. 2016. Antioxidant properties of selected varieties of lettuce (Lactuca sativa L.) commercially available in Malaysia. International Food Research Journal 23(6): 2357-2362.
Gazula A, Kleinhenz MD, Scheerens JC and Ling PP. 2007. Anthocyanin Levels in Nine Lettuce (Lactuca sativa) Cultivars: Influence of Planting Date and Relations among Analytic, Instrumented, and Visual Assessments of Color. HortScience 42(2): 232–238.
Gutierrez A, Herrera N, Castro L, Fernandez JM and Rodas FR. 2019 Low Cost Outdoors Climate Monitoring Network Using Zigbee Wireless Technology. Journal Revista Ibérica de Sistemas e Tecnologias de Informação. RISTI(19): 56-68
Hart G. 2005. The Routledge Dictionary of Egyptian Gods and Goddesses. Second edition. Psychology Press, Routledge. 170 p.
Henz GP e Suinaga F. 2009. Tipos de alface cultivados no Brasil. Comunicado Técnico No. 75. Embrapa Hortaliças, Brasília. 7 p.
Holeman E, Sentelhas PC and Mello SC. 2017. Cherry tomato yield in greenhouses with different plastic covers. Ciência Rural 47(10): e20160991. doi: 10.1590/0103-8478cr20160991
Hooper L and Cassidy A. 2006. A review of the health care potential of bioactive compounds. Journal of the Science of Food and Agriculture 86(12): 1805-1813. doi: 10.1002/jsfa.2599
Huang D, Ou B and Prior RL. 2005. The chemistry behind antioxidant capacity assays. Journal Agriculture Chemistry 53(6): 1841-1856. doi: 10.1021/jf030723c
Ilić SZ, Milenković L, Dimitrijević A, Stanojević L, Cvetković D, Kevrešan Ž, Fallik E and Mastilović J. 2017. Light modification by color nets improve quality of lettuce from summer production. Science Horticulture 226: 389-397. doi: 10.1016/j.scienta.2017.09.009
Kim DE, Shang X, Assefa AD, Keum YS and Saini RK. 2018. Metabolite profiling of green, green/red, and red lettuce cultivars: Variation in health beneficial compounds and antioxidant potential. Food Research International 105: 361–370. doi: 10.1016/j.foodres.2017.11.028
Kong JM, Chia LS, Goh NK, Chia TF and Brouillard R. 2003. Analysis and biological activities of anthocyanins. Phytochemistry 64(5): 923–933. doi: 10.1016/S0031-9422(03)00438-2
Křístková E, Doležalová I, Lebeda A, Vinter V and Novotná A. 2008. Description of morphological characters of lettuce (Lactuca sativa L.) genetic resources. Horticultural Science 35(3): 113–129. doi: 10.17221/4/2008-HORTSCI
Lebeda A, Ryder EJ, Grube R, Doležalová I And Křístková E. 2007. Lettuce (Asteraceae; Lactuca spp.). pp. 377–472. In: Singh RJ (ed.). Genetic Resources, Chromosome Engineering, and Crop Improvement. Vol. 3. CRC Press, Boca Ratón.
Li J, Wang X, Song W, Huang X, Zhou J, Zeng H, Sun S, Jia H, Li W, Zhou X, Li S, Chen P, Wu C, Guo Y, Han T and Qiu L. 2017. Genetic variation of maturity groups and four E genes in the Chinese soybean mini core collection. PLoS ONE 12(2): e0172106. doi: 10.1371/journal.pone.0172106
Liu X, Ardo S, Bunning M, Parry J, Zhou K, Stushnoff C, Stoniker F, Yu L and Kendall P. 2007. Total phenolic content and DPPH radical scavenging activity of lettuce (Lactuca sativa L.) grown in Colorado. LWT-Food Science and Technology 40(3): 552–557. doi: 10.1016/j.lwt.2005.09.007
Llorach R, Martínez-Sánchez A, Tomás-Barberán FA, Gil MI, Ferreres F. 2008. Characterisation of polyphenols and antioxidant properties of five lettuce varieties and escarole. Food Chemistry 108(3):1028-1038. doi: 10.1016/j.foodchem.2007.11.032
López A, Javier GA, Fenoll J, Hellín P and Flores P. 2014. Chemical composition and antioxidant capacity of lettuce: Comparative study of regular-sized (Romaine) and baby-sized (Little Gem and Mini Romaine) types. Journal of Food Composition and Analysis 33(1): 39-48. doi: 10.1016/j.jfca.2013.10.001
MADR-Ministerio De Agricultura Y Desarrollo Rural. 2014. Informe de la cadena de Hortalizas. En: https://es.scribd.com/document/400140766/006-Inf-Coyuntura-Actualidad-Cadena-Hortalizas-docx consulta: junio 2018.
MADR -Ministerio De Agricultura y Desarrollo Rural. 2006. Plan Hortícola Nacional. En: https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&ved=2ahUKEwj4k63Hvr3mAhVlrlkKHYIUDHcQFjAAegQIARAC&url=http%3A%2F%2Fwww.asohofrucol.com.co%2Farchivos%2Fbiblioteca%2Fbiblioteca_28_PHN.pdf&usg=AOvVaw0cZUPeq-Ur91lMvrMvcRXx consulta: junio 2018.
Mampholo BM, Maboko MM, Soundy P and Sivakumar D. 2016. Phytochemicals and Overall Quality of Leafy Lettuce (Lactuca sativa L.) Varieties Grown in Closed Hydroponic System. Journal of Food Quality 39(6): 805-815. doi: 10.1111/jfq.12234
Manach C, Scalbert A, Morand C, Rémésy C and Jiménez L. 2004. Polyphenols: food sources and bioavailability. The American Journal of Clinical Nutrition 79(5):727–747. doi: 10.1093/ajcn/79.5.727
Mello LD and Quadros GP. 2014. Correlation between antioxidant activity and total phenolic content with physicochemical parameters of blended extracts of Camellia sinensis. Acta Scientiarum 36(1):97-103. doi: 10.4025/actascihealthsci.v36i1.12615
Mesa-Vanegas AM, Zapata-Uribe S, Arana LM, Zapata IC, Monsalve Z y Rojano B. 2015. Actividad antioxidante de extractos de diferente polaridad de Ageratum conyzoides L. Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas 14(1): 1 – 10.
MinSalud – Ministerio de Salud y Protección social de Colombia. 2013. Perfil Nacional De Consumo De Frutas Y Verduras. In: MinSalud, https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&ved=2ahUKEwjHxIH6wL3mAhVOzlkKHQW7AXwQFjAAegQIARAC&url=https%3A%2F%2Fwww.minsalud.gov.co%2Fsites%2Frid%2FLists%2FBibliotecaDigital%2FRIDE%2FVS%2FPP%2FSNA%2Fperfil-nacional-consumo-frutas-y-verduras-colombia-2013.pdf&usg=AOvVaw133r7xYFQvlB5kahSlu4hb consulta: junio 2018
Mou B. 2008. Lettuce. pp. 75–116. In: Prohens J and Nuez F. (eds). Vegetables I. Asteraceae, Brassicaceae, Chenopodiaceae and Cucurbitaceae. Springer Science, New York. 428 p.
Nishimuro H, Ohnishi H, Sato M, Ohnishi-Kameyama M, Matsunaga I, Naito S, Ippoushi K, Oike H, Nagata T, Akasaka H, Saitoh S, Shimamoto K and Kobori M .2015. Estimated daily intake and seasonal food sources of quercetin in Japan. Nutrients 7(4): 2345–2358. doi:10.3390/nu7042345
Nicolle C, Carnat A, Fraisse D, Lamaison JL, Rock E, Michel H, Amouroux P and Remesy C. 2004. Characterisation and variation of antioxidant micronutrients in lettuce (Lactuca sativa folium). Journal Science Food Agriculture 84(15): 2061 – 2069. doi: 10.1002/jsfa.1916
Ntsoane LLM, Soundy P, Jifon J and Dharini S. 2016. Variety-specific responses of lettuce grown under the different coloured shade nets on phytochemical quality after postharvest storage. The Journal Horticultural Science and Biotechnology 91(5): 520-528. doi: 10.1080/14620
Ordidge M, García-Macías P, Battey NH, Gordon MH, Hadley P, John P, Lovegrove JA, Vysini E and Wagstaffe A. 2010. Phenolic contents of lettuce, strawberry, raspberry, and blueberry crops cultivated under plastic films varying in ultraviolet transparency. Food Chemistry 119(3): 1224–1227. doi: 10.1016/j.foodchem.2009.08.039
Ozgen S and Sekerci S, 2011. Effect of leaf position on the distribution of phytochemicals and antioxidant capacity among green and red lettuce cultivars. Spanish Journal of Agricultural Research 9(3): 801-809. doi: 10.5424/sjar/20110903-472-10
Paixão N, Perestrelo R, Marques JC and Câmara JS. 2007. Relationship between antioxidant capacity and total phenolic content of red, rosé and white wines. Food Chemistry 105(1): 204–214. doi: 10.1016/j.foodchem.2007.04.017
Pellegrini N, Serafini M, Colombi B, Del Rio D, Salvatore S, Bianchi M and Brighenti F. 2003. Total antioxidant capacity of plant foods, beverages and oils consumed in Italy assessed by three different in vitro assays. The Journal of Nutrition 133(9):2812-2819. doi: 10.1093/jn/133.9.2812
Shi X, Yu F, Jiang C, Wang H and Yukui R. 2015. Comparison of nutrient components of Chinese lettuce (Lactuca sativa L.) and American lettuce. Emirates Journal of Food and Agriculture 27(10): 790-792. doi: 10.9755/ejfa.2015-08-603
Singleton VL and Rossi JA. 1965. Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. American Journal of Enology and Viticulture 16: 144-158.
Sivakumar D, Jifon J and Soundy P. 2017. Spectral quality of photo-selective shade nettings improves antioxidants and overall quality in selected fresh produce after postharvest storage. Food Reviews International 34(3): 290-307. doi: 10.1080/87559129.2017.1298124
Sofo A, Lundegårdh B, Mårtensson A, Manfrac M, Pepe G, Sommella E, De Nisco M, Tenore GC, Campiglia P and Scopa A. 2016. Different agronomic and fertilization systems affect polyphenolic profile, antioxidant capacity and mineral composition of lettuce. Scientia Horticulturae 204: 106–115. doi: 10.1016/j.scienta.2016.04.003
Souza PF, Borghezan M, Zappelini J, Carvalho LR, Ree J, Barcelos-Oliveira JL and Pescador R. 2019. Physiological differences of ‘Crocantela’ lettuce cultivated in conventional and hydroponic systems. Horticultura Brasileira 37(1): 101-105. doi: 10.1590/S0102-053620190116
Steiner AA. 1984. The Universal Nutrient Solution. In: Sixth International Congress on Soilless Culture. Wageningen. pp. 633-650.
Tiveron AP, Melo PS, Bergamaschi KB, Vieira TMFS, Regitano-d’Arce MAB and Alencar SM. 2012. Antioxidant activity of Brazilian vegetables and its relation with phenolic composition. International Journal of Molecular Sciences 13(7): 8943–8957. doi: 10.3390/ijms13078943
Trejo-Téllez LI and Gómez-Merino FC. 2012. Nutrient Solutions for Hydroponic Systems. pp. 1-22. In: Asao T (ed.). Hydroponics - A Standard Methodology for Plant Biological Researches. InTech. 244 p.
USDA-United States Department of Agriculture. 2016. Vegetables and Pulses Year Data. In: Economic research service, https://www.ers.usda.gov/data-products/vegetables-and-pulses-data/ accessed: April 2016.
Vargas-Arcila M, Cartagena-Valenzuela JR, Franco G, Correa-Londoño GA, Quintero-Vásquez LM and Gaviria-Montoya CA. 2017. Changes in the physico-chemical properties of four lettuce (Lactuca sativa L.) varieties during storage. Ciencia y Tecnología Agropecuaria 18(2): 257-273. doi: 10.21930/rcta.vol18_num2_art:632
Zambrano-Moreno EL, Chávez-Jáuregui RN, Plaza ML and Wessel-Beaver L. 2015. Phenolic content and antioxidant capacity in organically and conventionally grown eggplant (Solanum melongena) fruits following thermal processing. Food Science and Technology (Campinas) 35(3): 414-420. doi: 10.1590/1678-457X.6656
Zhou YH, Zhang YY, Zhao X, Yu HJ, Shi K and Yu JQ. 2009. Impact of light variation on development of photoprotection, antioxidants, and nutritional value in Lactuca sativa L. Journal of Agriculture and Food Chemistry 57(12): 5494-5500. doi: 10.1021/jf8040325
How to Cite
License
Copyright (c) 2020 Revista Facultad Nacional de Agronomía Medellín

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The journal allows the author(s) to maintain the exploitation rights (copyright) of their articles without restrictions. The author(s) accept the distribution of their articles on the web and in paper support (25 copies per issue) under open access at local, regional, and international levels. The full paper will be included and disseminated through the Portal of Journals and Institutional Repository of the Universidad Nacional de Colombia, and in all the specialized databases that the journal considers pertinent for its indexation, to provide visibility and positioning to the article. All articles must comply with Colombian and international legislation, related to copyright.
Author Commitments
The author(s) undertake to assign the rights of printing and reprinting of the material published to the journal Revista Facultad Nacional de Agronomía Medellín. Any quotation of the articles published in the journal should be made given the respective credits to the journal and its content. In case content duplication of the journal or its partial or total publication in another language, there must be written permission of the Director.
Content Responsibility
The Faculty of Agricultural Sciences and the journal are not necessarily responsible or in solidarity with the concepts issued in the published articles, whose responsibility will be entirely the author or the authors.






