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Effect of Lactobacillus acidophilus added to a starch coating related to the microbiological contamination, quality and acceptability of fresh cheese
Efecto de Lactobacillus acidophilus añadido a un revestimiento de almidón en relación a la contaminación microbiológica, calidad y aceptabilidad de queso fresco
DOI:
https://doi.org/10.15446/rfnam.v74n3.90246Keywords:
Coated cheese , Inhibition, Salmonella, Satisfaction test, Sensory analysis (en)Queso recubierto, Inhibición , Salmonella, Prueba de satisfacción (es)
The fresh cheese produced in the province of Manabí is an Ecuadorian artisan cheese. The processing conditions commonly do not comply Ecuadorian regulations, resulting in the presence of pathogenic microorganisms such as Salmonella. The high number of cases of Salmonellosis in the province of Manabí justifies the need to identify and control the possible sources of this pathogenic microorganism. In the present work, the effect of the addition of Lactobacillus acidophilus to fresh cheese was studied, by immersing it in a starch solution with 1x108 CFU mL-1 of L. acidophilus with further storage for 30 days at 4 °C. The pH, acidity, weight loss, instrumental firmness, number of CFU of mesophilic aerobic bacteria and acceptability of fresh cheese were analyzed. At the same time, a duo-trio analysis was carried out, followed by a satisfaction analysis with the participation of semi-trained panelists. The presence of L. acidophilus reduced the pH and acidity in the fresh cheese in relation to the control sample. Satisfaction results, on a five-point hedonic scale, which ranged from 1 (I dislike it very much) to 5 (I like it very much), showed that the cheese treated with L. acidophilus and the control sample received a rating between “I neither like nor dislike” and “I like it moderately”, with values of 3.63 for the treated sample and 3.50 for the control. The application of L. acidophilus did not affect the organoleptic acceptability of cheese and produced less changes in pH, acidity and weight loss during storage for 30 days at 4 °C in relation to the control sample.
El queso fresco producido en la provincia de Manabí es un queso artesanal ecuatoriano, cuyas condiciones de procesamiento comúnmente no cumplen con la normativa ecuatoriana, dando como resultado la presencia de microorganismos patógenos como la Salmonella. El alto número de casos de Salmonelosis en la provincia de Manabí justifica la necesidad de identificar y controlar las posibles fuentes de este microorganismo patógeno. En el presente trabajo se estudió el efecto de la adición de Lactobacillus acidophilus al queso fresco, mediante la inmersión de este en una solución de almidón con 1x108 UFC mL-1 de L. acidophilus y su posterior almacenamiento por 30 días a 4 °C, durante los cuales se analizó el pH, acidez, pérdida de peso, firmeza instrumental, número de UFC de bacterias aerobias mesófilas y satisfacción del queso fresco. Al mismo tiempo se realizó un análisis dúo-trío y seguidamente uno de satisfacción con la participación de panelistas semientrenados. La presencia de Lactobacillus acidophilus redujo el pH y acidez en el queso fresco en relación con la muestra control. Los resultados de satisfacción, en una escala hedónica de cinco puntos, que varió desde 1 (me desagrada mucho) a 5 (me agrada mucho), mostraron que el queso tratado con L. acidophilus y la muestra control recibieron una calificación entre “ni me gusta ni me disgusta” y “me gusta moderadamente”, con valores de 3.63 para la muestra tratada y 3.50 para el control. La aplicación de L. acidophilus no afectó el nivel de agrado de los panelistas por el queso fresco y produjo menores cambios de pH, acidez y pérdida de peso durante su almacenamiento por 30 días a 4 °C en relación a la muestra control.
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