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Effect of different concentrations of indole butyric acid, putrescine and hydrogen peroxide on stem cuttings of the rootstock GF677(Prunus amygdalus × Prunus persica) according to the cutting season
Efecto de diferentes concentraciones de ácido indol butírico, putrescina y peróxido de hidrógeno en el enraizamiento de esquejes de tallo de durazno GF677 (Prunus amygdalus × Prunus persica) según las temporadas de corte
DOI:
https://doi.org/10.15446/rfnam.v74n2.92414Keywords:
Adventitious rooting, semi-hardwood cuttings, carbohydrate, woody cuttings, cofactors (en)Enraizamiento adventicio, esquejes de madera semidura, carbohidratos, esquejes leñosos, cofactores (es)
The rootstock GF677 is an interspecific hybrid with an important economic and horticultural value. In this research, the effect of indole butyric acid (IBA) in combination with putrescine (Put) and hydrogen peroxide (H2O2) on rooting of GF677 semi-hardwood stem cuttings in three cutting seasons (July, March and October) was investigated. Treatments as IBA (0, 1000, 2000 and 3000 mg L-1), Put (0, 800, 1600 and 3200 mg L-1) and H2O2 (1.5, 3 and 6% w/v) were included. The results showed that in July cuttings, the highest levels of callogenesis were observed in IBA treated cuttings in both concentrations of 1000 and 2000 mg L-1. The rooting was very low in July cuttings, while the highest percentage of rooting (14%) was observed in the combination of 2000 mg L-1 IBA+ 3% H2O2. In March, the cuttings treated by 1000 mg L-1 IBA+800 mg L-1 Put and 1000 mg L-1 IBA+1600 mg L-1 Put revealed the highest percentages of callus formation 83.31 and 83.33%, respectively. In these cuttings, the highest percentage of rooting (63.88%) was gained at 2000 mg L-1 IBA+3200 mg L-1 Put. The application of 1000 mg L-1 IBA+800 mg L-1 Put increased root fresh weight. In cuttings prepared in October, only 800 mg L-1 Put caused callus formation in more than 55% of the cuttings. The rooting of cuttings at this time was as low as the July cuttings, whereas the highest rooting percentage was observed in cuttings treated with IBA at a concentration of 1000 mg L-1. Overall, the experiment showed that the season of the cutting and the treatments with IBA+Put or H2O2 could improve rooting properties of the rootstock GF677.
El portainjerto GF677 es un híbrido interespecífico con un importante valor económico y hortícola. En este estudio, se investigó el efecto del ácido indol butírico (IBA) en combinación con putrescina (Put) y peróxido de hidrógeno (H2O2) en el enraizamiento de esquejes de tallos de madera semidura GF677 en tres temporadas de corte (julio, marzo y octubre). Los tratamientos incluyeron IBA (0, 1000, 2000 y 3000 mg L-1), Put (0, 800, 1600 y 3200 mg L-1) y H2O2 (1.5, 3 y 6% p/v). Los resultados mostraron que en los esquejes de julio, la callogénesis más alta se observó en los esquejes tratados con IBA en ambas concentraciones de 1000 y 2000 mg L-1. El enraizamiento fue muy bajo en los esquejes de julio y el mayor porcentaje de enraizamiento (14%) se observó en la combinación de IBA 2000 mg L-1+H2O2 3%. En los esquejes de marzo, el mayor porcentaje de formación de callos se alcanzó en los tratados con IBA 1000 mg L-1+Put 800 mg L-1 e IBA 1000 mg L-1+Put 1600 mg L-1, 83.31 y 83.33%, respectivamente. En estos esquejes, el mayor porcentaje de enraizamiento (63.88%) se obtuvo con IBA 2000 mg L-1+Put 3200 mg L-1. La aplicación de IBA 1000 mg L-1+Put 800 mg L-1 aumentó el peso fresco de la raíz. En esquejes preparados en octubre, el Put solo a una concentración de 800 mg L-1provocó la formación de callos en más del 55% de los esquejes. El enraizamiento de los esquejes en este momento era tan bajo como los esquejes de julio y el mayor porcentaje de enraizamiento se observó en los esquejes tratados con IBA a una concentración de 1000 mg L-1. En general, el experimento mostró que la temporada de corte y el tratamiento con IBA+Put o H2O2 podría mejorar las propiedades de enraizamiento de los esquejes de GF677.
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1. Romel S. Romano, Shierel F. Vallesteros, Romnick L. Pascua . (2024). Vegetative propagation of Bagoadlau (Xanthosthemon philippinensis Merr.) using indolebutyric acid . Journal of Degraded and Mining Lands Management, 11(4), p.6081. https://doi.org/10.15243/jdmlm.2023.114.6081.
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