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Bioactive compounds against Moniliophthora roreri (Cif & Par) identified in locally produced liquid amendments (Biols)
Compuestos bioactivos contra Moniliophthora roreri (Cif & Par) identificados en enmiendas líquidas producidas localmente (Bioles)
DOI:
https://doi.org/10.15446/rfnam.v76n2.99365Keywords:
Agriculture, Biopreparations, Fermentative process, Phytopathogens, Plaguicide (en)Agricultura, Biopreparados, Proceso fermentativo, Fitopatógenos, Plaguicidas (es)
The use of liquid organic amendments (biols) is a common practice for farmers due to the multiple benefits in the management and production of their crops, including the control of pests and diseases. The present study analyzes the chemical composition of the pure compound C1 and fractions F2 and F3 of local biols produced in two provinces of Ecuador and their antifungal activity against Moniliophthora roreri (Cif & Par). This work incorporates the use of Gas Chromatography coupled to Mass Spectrometry (GC-MS), Nuclear Magnetic Resonance (NMR) and in vitro inhibition assays for sample analysis. C1 was identified as Mannitol. The percentage of inhibition against M. roreri in F2 and F3 was 44.37% and 8.34%, respectively; and, for C1, 28.63%. The values of the median lethal dose (LD50) obtained corroborate that the F2 fraction was the one with the highest controlling activity against the pathogen. The 1,2-benzenedicarboxylic acid diisooctyl ester compound was the main compound in F2 (30.88%) and the Pentadecanoic acid, 14-methyl-, methyl ester in F3. Finally, all compounds obtained from the biol fractions were identified and it was determined that the fermentative process was suitable for producing bioactive compounds of interest to inhibit the growth of Moniliophthora roreri.
El uso de enmiendas orgánicas líquidas (bioles) es una práctica común para los agricultores por los múltiples beneficios en el manejo y producción de sus cultivos, incluyendo el control de plagas y enfermedades. El presente estudio analiza la composición química del compuesto puro C1 y fracciones F2 y F3 de bioles locales producidos en dos provincias del Ecuador y su actividad antifúngica frente a Moniliophthora roreri (Cif & Par). Este trabajo incorpora el uso de Cromatografía de Gases acoplado a Espectrometría de Masas (CG-EM), Resonancia Magnética Nuclear (RMN) y ensayos de inhibición in vitro para el análisis de las muestras. C1 fue identificado como Manitol. El porcentaje de inhibición contra M. roreri en F2 y F3 fue del 44,37% y 8,34% respectivamente; y de C1, 28,63 %. Los valores de las dosis letales media (DL50) obtenidos corroboraron que la fracción F2 fue la que mayor actividad controladora tuvo frente al patógeno. Los compuestos éster diisooctil del ácido 1,2-bencenicarboxílico fue el compuesto mayoritario en F2 (30,88%) y el ácido pentadecanoico, 14-metil-, éster metílico en F3. Finalmente, se identificaron todos los compuestos de las fracciones obtenidas a partir de biol, y se determinó que el proceso fermentativo era adecuado para producir compuestos bioactivos de interés para inhibir el crecimiento de Moniliophthora roreri.
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