Effect of silymarin on oxidative damage in chicken liver cell membranes
Efecto de la silimarina sobre el daño oxidativo en membranas celulares de hígado de pollo
DOI:
https://doi.org/10.15446/rfmvz.v70n1.102246Keywords:
silymarin, liver, chicken, chemiluminescence (en)silimarina, hígado, pollo, quimioluminiscencia (es)
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In living beings, antioxidants are of vital importance for protection against oxidative damage caused by reactive oxygen species. Silymarin (SM), a plant-derived flavonoid present in the fruits and seeds of milk thistle Silybum marianum (L.) Gaertn., has a recognized hepatoprotective effect. In this work, the in vitro silymarin antioxidant effect on non-enzymatic peroxidation (NEP) in chicken liver mitochondria and microsomes was studied. Oxidative stress in the organelles was induced by subjecting the samples (1 mg of protein) to an ascorbate-Fe++-dependent prooxidant system at 37 °C. Oxidative damage was quantified by chemiluminescence (CL) using a Packard1900 TR liquid scintillation counter (Meriden CT, USA). CL expressed as cpm (counts per minute) was read every 10 minutes to establish the course of peroxidation as a function of time. Likewise, the total cpm value (sum of the readings) was used to compare the inhibitory effect of SM using different concentrations corresponding to 6.25; 12.5, and 25 μg of the active ingredient (silymarin phosphatide) per mg of mitochondrial and microsomal protein. Controls were run simultaneously without the addition of ascorbate. Peroxidation inhibition was dependent on the concentration of SM in the incubation mixture. The results show that a protective effect on induced oxidative damage was found for all concentrations tested.
En los seres vivos, los antioxidantes son de vital importancia para la protección contra el daño oxidativo causado por las especies reactivas del oxígeno. La silimarina (SM), un flavonoide de origen vegetal presente en los frutos y semillas del cardo mariano Silybum marianum (L.) Gaertn., tiene un reconocido efecto hepatoprotector. En este trabajo se estudió el efecto antioxidante in vitro de la silimarina sobre la peroxidación no enzimática (PNE) en mitocondrias y microsomas de hígado de pollo. El estrés oxidativo en los orgánulos se indujo sometiendo las muestras (1 mg de proteína) a un sistema prooxidante dependiente de ascorbato-Fe++ a 37 °C. El daño oxidativo se cuantificó por quimioluminiscencia (QL) utilizando un contador de centelleo líquido Packard 1900 TR (Meriden CT, EE. UU.). QL expresado como cpm (recuentos por minuto) se leyó cada 10 minutos para establecer el curso de la peroxidación en función del tiempo. Asimismo, se usó el valor total de cpm (suma de las lecturas) para comparar el efecto inhibitorio de SM mediante diferentes concentraciones correspondientes a 6,25; 12,5 y 25 μg del ingrediente activo (fosfátido de silimarina) por mg de proteína mitocondrial y microsomal. Los controles se realizaron simultáneamente sin la adición de ascorbato. La inhibición de la peroxidación dependía de la concentración de SM en la mezcla de incubación. Los resultados muestran que para todas las concentraciones probadas se encontró un efecto protector sobre el daño oxidativo inducido.
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