Published

1971-04-01

Método para la purificación del virus herpes simplex

Keywords:

herpesvirus, virus del herpes simple (es)

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Authors

  • Julio Enrique Ospina Universidad Nacional de Colombia
Se propone en el presente trabajo un procedimiento técnico para la purificación de virus herpes simplex por medio de centrifugación diferencial y gradientes de sucrosa en una concentración del 40 por ciento al 20 por ciento y centrifugación final a 40000 g. El 90 por ciento de las partículas obtenidas presentaron alrededor de sus capsides formaciones membranosas similares a las descritas en la literatura como de origen celular y naturaleza lipoproteica. Un 10 por ciento de las partículas se obtuvieron desnudas, algunas con sus capsides rotas. Se usó virus herpes hominis, cepa HF, en células de riñon de conejo. La infectividad de las partículas se comprobó por medio de técnicas inmunofluorescentes.

How to Cite

APA

Ospina, J. E. (1971). Método para la purificación del virus herpes simplex. Revista de la Facultad de Medicina, 37(2), 148–158. https://revistas.unal.edu.co/index.php/revfacmed/article/view/22095

ACM

[1]
Ospina, J.E. 1971. Método para la purificación del virus herpes simplex. Revista de la Facultad de Medicina. 37, 2 (Apr. 1971), 148–158.

ACS

(1)
Ospina, J. E. Método para la purificación del virus herpes simplex. Rev. Fac. Med. 1971, 37, 148-158.

ABNT

OSPINA, J. E. Método para la purificación del virus herpes simplex. Revista de la Facultad de Medicina, [S. l.], v. 37, n. 2, p. 148–158, 1971. Disponível em: https://revistas.unal.edu.co/index.php/revfacmed/article/view/22095. Acesso em: 5 aug. 2024.

Chicago

Ospina, Julio Enrique. 1971. “Método para la purificación del virus herpes simplex”. Revista De La Facultad De Medicina 37 (2):148-58. https://revistas.unal.edu.co/index.php/revfacmed/article/view/22095.

Harvard

Ospina, J. E. (1971) “Método para la purificación del virus herpes simplex”, Revista de la Facultad de Medicina, 37(2), pp. 148–158. Available at: https://revistas.unal.edu.co/index.php/revfacmed/article/view/22095 (Accessed: 5 August 2024).

IEEE

[1]
J. E. Ospina, “Método para la purificación del virus herpes simplex”, Rev. Fac. Med., vol. 37, no. 2, pp. 148–158, Apr. 1971.

MLA

Ospina, J. E. “Método para la purificación del virus herpes simplex”. Revista de la Facultad de Medicina, vol. 37, no. 2, Apr. 1971, pp. 148-5, https://revistas.unal.edu.co/index.php/revfacmed/article/view/22095.

Turabian

Ospina, Julio Enrique. “Método para la purificación del virus herpes simplex”. Revista de la Facultad de Medicina 37, no. 2 (April 1, 1971): 148–158. Accessed August 5, 2024. https://revistas.unal.edu.co/index.php/revfacmed/article/view/22095.

Vancouver

1.
Ospina JE. Método para la purificación del virus herpes simplex. Rev. Fac. Med. [Internet]. 1971 Apr. 1 [cited 2024 Aug. 5];37(2):148-5. Available from: https://revistas.unal.edu.co/index.php/revfacmed/article/view/22095

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