Técnica para obtener deyecciones hialianas como inoculo y para microanálisis, con ninfas de quinto estadio de R. prolixus, infectadas y normales
Keywords:
deyectograma, Rhodnius prolixus, ninfas de quinto estadio, Schizotrypanum cruzi, inóculo (es)fifth instar nymphs, inoculum (en)
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Se describe una técnica original para colectar, en buenas condiciones, deyecciones hialinas para microanálisis o para inoculo, seleccionando la muestra según el "Deyectograma". Se dan detalles para la fijación de los ejemplares en el mondadientes y la colocación de éstos en el dispositivo para recoger la muestra. Se anotan las ventajas del inóculo hialino con otros métodos de infección experimental. Se anota la posibilidad de hacer cultivos de formas meta cíclicas de S. cruzi, ampliando el número de experimentos, que en el presente estudio es insuficiente. Para fines micro analíticos de las excretas pastosas, se describe otro dispositivo para la recolección de las muestras, según el estudio del "Deyectograma", en cuanto a colorido y duración
.A new original technique for collecting is described, with favorable conditions, hyaline dejections for microscopie analysis or for inoculation, selecting the type according to the "Deyec tograma". Details are given for the placing of the samples on the toothpick and the collection of these in the dispositive for gathering the sample. The advantages of hyaline inoculation with other methods of experimental infection are noted. To get micro-analytical pasty excretions another dispositive is described, for the gathering of samples according to the study of "Deyectograma", as far as coloring and duration.
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