Publicado

2017-10-01

Asymbiotic seed germination and in vitro propagation of Cattleya trianae Linden & Reichb.f. (Orchidaceae)

Germinación asimbiótica de semillas y propagación in vitro de Cattleya trianae Linden y Reichb.f. (Orchidaceae)

Palabras clave:

Organic additives, coconut water, Pineapple juice, in vitro propagation (en)
Organic additives, coconut water, Pineapple juice, in vitro propagation (es)

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Autores/as

  • Seir Antonio Salazar Mercado Facultad de Ciencias Agrarias y del Medio Ambiente, grupo de Investigación Ambiente y Vida, semillero Grupo Académico de Investigaciones Agrobiotecnológicas (GAIA), Universidad Francisco de Paula Santander. Avenida Gran Colombia No. 12E-96B Colsag. San José de Cúcuta, Colombia https://orcid.org/0000-0002-3287-703X
  • Nelson Alfonso Vega Contreras Facultad de Ciencias Agrarias y del Medio Ambiente, grupo de Investigación Ambiente y Vida, semillero Grupo Académico de Investigaciones Agrobiotecnológicas (GAIA), Universidad Francisco de Paula Santander. Avenida Gran Colombia No. 12E-96B Colsag. San José de Cúcuta, Colombia
Cattleya trianae (Linden & Reichb.f., 1860), Colombian national flower, is in danger of extinction due to the destruction of its natural habitats and excessive collection for horticultural purposes. Therefore, in vitro culture is a tool for the conservation of threatened species. In this study we determined the most suitable culture medium for asimbytic seed germination and in vitro propagation of C. trianae. Initially, mature capsules were collected, the seeds were subsequently disinfected and seeded with the syringe method (Vendrame et al., 2007), to evaluate the effect of five media on the development of C. trianae after 20 weeks. The seedlings were transplanted and acclimated using different substrates. The best percentage (54.2%) of seedling formation after 20 weeks was found in MS + JP medium with significant differences (P <0.05: Tukey HSD). In this research, it is reported that the addition of organic additives to the MS medium improves the efficacy of this, and therefore, allows a greater growth and development of C. trianae under in vitro conditions.

Cattleya trianae (Linden & Reichb.f., 1860), Colombian national flower, is in danger of extinction due to the destruction of its natural habitats and excessive collection for horticultural purposes. Therefore, in vitro culture is a tool for the conservation of threatened species. In this study we determined the most suitable culture medium for asimbytic seed germination and in vitro propagation of C. trianae. Initially, mature capsules were collected, the seeds were subsequently disinfected and seeded with the syringe method (Vendrame et al., 2007), to evaluate the effect of five media on the development of C. trianae after 20 weeks. The seedlings were transplanted and acclimated using different substrates. The best percentage (54.2%) of seedling formation after 20 weeks was found in MS + JP medium with significant differences (P <0.05: Tukey HSD). In this research, it is reported that the addition of organic additives to the MS medium improves the efficacy of this, and therefore, allows a greater growth and development of C. trianae under in vitro conditions.

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Citas

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