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Antifungal activity of acidic electrolyzed water against strawberry postharvest molds (Fragaria x ananassa Duch cv. Camarosa)
Actividad antifúngica del agua electrolizada ácida contra mohos postcosecha de fresa (Fragaria x ananassa Duch cv. Camarosa)
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https://doi.org/10.15446/acag.v68n2.78247Palabras clave:
Acidic Electrolyzed Water, antifungal, Botrytis cinerea, Postharvest Molds, Rhizopus stolonifer. (en)Agua ácida electrolizada, antifúngicos, Botrytis cinerea, Mohos Poscosecha, Rhizopus stolonifer (es)
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The aim of this word was to assess the in vitro antifungal activity of acidic electrolyzed water (AEW) and the required contact time to inhibit the development of post-harvest molds common in strawberry fruit. Ninety-four strawberry samples were used. A five-fold assay in a randomized design was carried out using standard inoculum of fungal conidia, which were subjected to an array of contact times and AEW concentrations, using a controlled agitation (120 r.p.m) at 23°C. The conidia treated with AEW were grown on potato dextrose agar and their germination was evaluated. Mycelial growth of the treated samples was measured and compared against conidia control samples treated with distilled water instead of AEW. Botrytis cinerea and Rhizopus stolonifer were the most commonly isolated species at 54.25% and 28.72%, respectively. AEW inhibited conidia germination after 7 minutes of exposure. The AEW concentration required to inhibit conidia germination was 10 ppm and 50 ppm for R. stolonifer and B. cinerea respectively, a variance possibly due to the constitution of their cellular walls. A short exposure of AEW is able to inhibit post-harvest fungi growth, and is thus recommended as an efficient, economical, and environmentally sustainable sanitation agent, with the ability of extending the strawberry post-harvest consumption period.
El objetivo de este trabajo fue evaluar la actividad antifúngica in vitro del agua electrolizada ácida (AEA) y el tiempo de contacto requerido para inhibir el desarrollo de mohos postcosecha comunes en fresa. Se utilizaron 94 frutos dañados y sanos. En un ensayo al azar con cinco repeticiones, utilizando un inóculo estándar de conidios fúngicos, tratados durante una serie de tiempos de contacto y diferentes concentraciones de AEA, utilizando una agitación controlada (120 r.p.m) a 23 °C. Los conidios tratados con AEA se cultivaron en agar papa-dextrosa para evaluar su germinación. Se midió el crecimiento micelial de las muestras tratadas y se comparó con las muestras de control de conidias tratadas con agua destilada en lugar de AEA. Botrytis cinerea y Rhizopus stolonifer fueron las especies más comúnmente aisladas con 54.25% y 28.72%, respectivamente. AEA inhibió la germinación de conidios después de 7 minutos de exposición. La concentración de AEA requerida para inhibir la germinación de los conidios fue de 10 ppm y 50 ppm para R. stolonifer y B. cinerea, respectivamente, las diferencias podrían estar relacionadas con la constitución de sus paredes celulares. Una exposición corta de AEA es capaz de inhibir el crecimiento de hongos poscosecha y, por tanto, con base en los resultados en este trabajo es posible recomendar al AEA como higienizante eficiente, económico y ambientalmente sostenible, para uso en frutos poscosecha y así prolongar el periodo de consumo.
Referencias
Ainsworth, G.C.; Sparrow, F.K.; Sussman, A.S. 1973. The Fungi: an advanced treatise. Academic Press, New York 4A:1-621.
Al-Haq, M.I.; Sugiyama, J.; Isobe, S. 2005. Applications of Electrolyzed Water in Agriculture and Food Industries. Food Sci. Technol. Res. 11 (2):135-150. https://doi.org/10.3136/fstr.11.135
Barnes, I.; Roux, J.; Wingfield, M.J.; Coetzee, MPA. 2001. Characterization of Seiridium spp. associated with cypress canker based on p-tubulin and histone sequences. Plant Dis.85:317-321. https://apsjournals.apsnet.org/doi/10.1094/PDIS.2001.85.3.317
Bialka, K.L.; Demirci, A.; Knabel, S.J.; Patterson, P.H.; Puri, V.M. 2004 Efficacy of electrolyzed oxidizing water for the microbial safety and quality of eggs. J Poult Sci. 83(12): 2071-2078. https://doi.org/10.1093/ps/83.12.2071
Cao, W.; Zhu, Z.W.; Shi, Z.X.; Wang, C.Y.; Li, B.M 2009. Efficiency of slightly acidic electrolyzed water for inactivation of Salmonella Enteritidis and its contaminated shell eggs. Int J Foof Microbiol. 130(2):88-93. https://doi.org/10.1016/j.ijfoodmicro.2008.12.021
Cary, J.W.; Beltz, S.B.; Bennett, CA. 2003 Identification of Common Aspergillus Species. Utrecht, Netherlands: Centraalbureau voor Schimmelcultures. 116 pp.
Coley-Smith, J.R. 1980 Sclerotia and other structures in survival. In: Coley-Smith JR, Verhoeff K and Jarvis WR (eds) The Biology of Botrytis. 85-114. Academic Press, London, UK
Callejas, T.A.; Martínez, G.B.; Artes, F.; Artes, H. F 2010. Neutral and acid electrolyzed water as emergent sanitizer for fresh-cut mizuna baby levels. Postharvest Biol Technol. 59(3):298-306. https://doi.org/10.1016/j.postharvbio.2010.09.013
Cui, X.; Shang, Y.; Shi, Z.; Xin, H.; Cao, W 2009. Physicochemical properties and bactericidal efficiency of neutral and acidic electrolyzed water under different storage conditions. J. Food Eng. 91, 582-586. https://doi.org/10.1016/j.jfoodeng.2008.10.006
Cravero, F.; Englezos, V.; Torchio, F.; Giacosa, S.; Segade, S. R.; Rantsiou, V. G. K.; Rolle, L. and Cocolin, L. 2016. Post-harvest control of wine- grape mycobiota using electrolyzed water. Inn. Food Sci. Emer. Technol. 35:21-28. https://doi.org/10.1016/j.ifset.2016.03.010
Dean, R.; Van Kan, J.A.; Pretorius, Z.A.; Hammond- Kosack K.E.; Di Pietro, A.; Spanu, P.D.; Rudd, J.J.; Dickman, M.; Kahmann, R.; Ellis, J and Foster GD 2012. The Top 10 fungal pathogens in molecular plant pathology. Mol. Plant Pathol. 13(4):414-430. https://doi.org/10.1111/j.1364-3703.2011.00783.x
Domsch, K.H.; Gams, W and Anderson, T.H. 1980. Compendium of Soil Fungi, 1-2: 1308. Acad Press, New York.
Feliziani, E.; Lichterb, A.; Smilanickc, J. L. and Ippolito, A. 2016. Disinfecting agents for controlling fruit and vegetable diseases after harvest. Postharvest Biol Technol . 122:53-69. https://doi.org/10.1016/j.postharvbio.2016.04.016
Fernández, A.F.J.; Carbú, M.; Garrido, C.; Vallejo, I.; Cantoral J.M. 2007 Proteomic advances in phytopathogenic fungi. Curr Proteomics. 4:79-88. https://doi.org/10.3390/ijms12010795
Guentzel, J. L.; Callan, M. A.; Lam, K. L.; Emmons, S. A.; and Dunham, V. L. 2011. Evaluation of electrolyzed oxidizing water for phytotoxic effects and pre-harvest management of gray mold disease on strawberry plants. Crop Prot. 30(10):1274-1279. https://doi.org/10.1016/j.cropro.2011.05.021.
Hao, J.; Qui, S.; Li, H.; Cheng, T.; Liu, H.; Li, L. 2012. Roles of hidroxyl radical in electrolyzed oxidizing water (EOW) for the inactivation of Escherichia coli. Int. J. Food Microbiol. 155(3):99-104. https://doi.org/10.1016/j.ijfoodmicro.2011.12.031
Holz, G.; Coertze, S.; Williamson, B. 2007. The ecology of Botrytis onplant surfaces. Eds (Elad, Y. ; Williamson, B. ; Tudzynski, P.; Denle, N) In: Botrytis: Biology, Pathology and Control. Heidelberg: Springer.9-27. https://doi.org/10.1007/978-1-4020-2626-3_2.
Huang, Y.R.; Hung, Y.C.; Hsu, S.Y.; Huang, Y.W.; and Hwang D.F (2008). Application of electrolyzed water in the food industry. Food Control. 19(4):329-345. https://doi.org/10.1016/j.foodcont.2007.08.012
Korsten, L.; Wehner, F.C. 2003. Fungi, In: Bartz, J.A.; Brecht, J.K. (Eds.), Postharvest Physiology and Pathology of Vegetables. Second edition Marcel Dekker, Inc., New York, 485-518.
Koseki, S.; Yoshida, K.; Isobe, S.; & Itoh, K. 2004. Efficacy of acidic electrolyzed water for microbial decontamination of cucumbers and strawberries. J. Food Prot. 67(6): 1247-1251. https://doi.org/10.4315/0362-028X-67.6.1247.
Lopes, U.P. 2011. Podridoes em pós-colheita de morango: Etiologia e efeito de produtos a alternativos. MSc Dissertation, Universidade Federal de Vigosa. Vigosa, MG. http://locus.ufv.br/bitstream/handle/123456789/4399/texto%20completo.pdf?sequence=1&i.
Nelson, P.E.; Toussoun, T.A.; and Marasas, W.F.O. 1983. Fusarium Species: An Illustrated Manual for Identification. The Penn. St. Univ.Press, Univ. Park, Pennsylvania. 203.
Newmeyer, D. 1990 Filtering small quantities of conidial suspensions to remove mycelial fragments, Fungal Genetics Reports: Vol. 37, Article 15. https://doi.org/10.4148/1941-4765.1480
O’Donnell, K.L. 1979. Zygomycetos in culture. Univ. Georgia Press, Georgia EE. UU., 257
Park, C.M.; Hung, Y.C. and Kim, C. 2002. Efectiveness of electrolyzed water as a sanitizer for treating diferent surfaces. J. Food Prot . 65:1276-1280 https://doi.org/10.4315/0362-028X-65.8.1276
Rigotti, S. ; Gindro, K. ; Richter, H.; Viret, O. 2002. Characterization of molecular markers for specific and sensitive detection of Botrytis cinérea Pers.: Fr. in strawberry (Fragaria x ananassa Duch.) using PCR. FEMS Microbiol Lett. 209:169-174. https://doi.org/10.1016/S0378-1097(02)00491-3
Seifert, K.A.; Gams, W. 2011 The genera of hyphomycetes. Persoonia. 27:119-129. https:/doi.org/10.3767/003158511X617435
White T.J.; Bruns, T.; Lee, S.; Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: a guide to methods and applications. (Innis, M.A. ; Gelfand, D.H. ; Sninsky, J.J. ; White, T.J, eds). Academic Press, San Diego, U.S.A.: 315-322
Zacharia, I.A.; Kamitani, Y.; Morita, K.; Iwasaki, K. 2010. Sanitization potency of slightly acidic electrolyzed water against pure cultures of Escherichia coli and Staphylococcus aureus, in comparison with that of other food sanitizers. Food Control 21(5):740-745. https://doi.org/10.1016/j.foodcont.2009.11.002
Zhou, Y.J.; Zhang, J.; Wang, X.D.; Yang., L.; Jiang , D.H..; Li , G.Q..; Hsiangand, T.; Zhuang, W.Y.. 2014. Morphological and phylogenetic identification of Botrytis sinoviticola, a novel cryptic species causing gray mold disease of table grapes (Vitis vinifera) in China. Mycologia 106:1.43-56. https://doi.org/10.3852/13-032
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