Publicado

2024-03-19

Comparison of maize performance between doubled haploids and different selfing generations in the Mexican subtropics

Comparación del rendimiento del maíz entre dobles haploides y diferentes generaciones de autofecundación en el subtrópico mexicano

DOI:

https://doi.org/10.15446/acag.v72n2.85349

Palabras clave:

double haploid, homozygosis, line by tester, maize, yield trails (en)
homocigosis, línea por probador, maíz, pruebas de rendimiento (es)

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Maize (Zea mays) hybrid breeding programs are increasingly adopting double haploid (DH) technology to enhance efficiency and meet the growing demand for food in the face of population growth and climate change. However, in developing countries such as those in subtropical regions, the utilization of DH technology is still limited. This study aims to evaluate the potential benefits of incorporating this technology into both new and established maize breeding programs. Using a pedigree selfing population from the CIMMYT Genebank, DH lines were developed and compared to the conventional pedigree selfing method through a line-by-tester evaluation. Fifteen DH lines and fourteen lines from each of the S2, S4, and S6 selfing generations were subjected to multilocation yield trials in the subtropics of Mexico. The results showed that it took three years for the S1 population to identify DH lines with 100 % homozygosity and the highest general combining ability (GCA), which was one year earlier than the S6 lines (98,4 % homozygous). DH lines exhibited more genotype-by-environment interactions (GxE) compared to S2 testcrosses but demonstrated similar heritability to the S6 generation. Significant statistical differences were observed between the DH evaluations and the S4 and S6 testcrosses, while no significant difference was found between the S2 and DH evaluations. Based on these findings, a gradual implementation of DH technology in hybrid breeding programs is recommended. However, the conventional pedigree method should not be abandoned entirely, and testing in selfing generations, particularly in S6, remains crucial. This study provides a statistical comparison of DH versus the conventional pedigree method using a line-by-tester approach, contributing valuable insights for plant breeders and researchers in the field of maize breeding.

Los programas de fitomejoramiento de maíz (Zea mays) híbrido están adoptando cada vez más la tecnología de dobles haploides (DH) para mejorar la eficiencia y satisfacer la creciente demanda de alimentos en el contexto del crecimiento de la población y el cambio climático. Sin embargo, en países en desarrollo, como aquellos en regiones subtropicales, la utilización de la tecnología DH aún es limitada. Este estudio tiene como objetivo evaluar los posibles beneficios de incorporar la tecnología DH en programas de mejoramiento de maíz, tanto nuevos como establecidos. Utilizando una población de autofecundación de pedigrí proveniente del Banco de Germoplasma CIMMYT, se desarrollaron líneas DH y se compararon con el método convencional de autofecundación de pedigrí a través de una evaluación línea por probador. Quince líneas DH y catorce líneas de cada una de las generaciones de autofecundación S2, S4 y S6 se sometieron a ensayos de rendimiento en múltiples localidades de la región subtropical de México. Los resultados mostraron que se necesitaron tres años en la población S1 para identificar líneas DH con 100 % de homocigosidad y la mayor habilidad combinatoria general (HCG), lo cual ocurrió un año antes que las líneas S6 (98,4 % homocigotas). Las líneas DH mostraron más interacciones genotipo por ambiente (GxE) en comparación con los híbridos S2, pero demostraron una heredabilidad similar a la generación S6. Se encontraron diferencias estadísticamente significativas entre las evaluaciones DH y los híbridos S4 y S6, mientras que no se encontraron diferencias significativas entre las evaluaciones S2 y DH. Con base en estos hallazgos, se recomienda una implementación gradual de la tecnología DH en programas de mejoramiento de híbridos. Sin embargo, el método convencional de pedigrí no debe ser abandonado por completo y las pruebas en generaciones de autofecundación, especialmente en S6, siguen siendo fundamentales. Este estudio proporciona una comparación estadística de DH versus el método convencional de pedigrí utilizando un enfoque línea por probador, aportando ideas valiosas para los fitomejoradores de plantas e investigadores en el campo del mejoramiento del maíz.

Referencias

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Cómo citar

APA

Vargas Escobar, E. A. y García Dávila, M. A. (2024). Comparison of maize performance between doubled haploids and different selfing generations in the Mexican subtropics. Acta Agronómica, 72(2), 168–175. https://doi.org/10.15446/acag.v72n2.85349

ACM

[1]
Vargas Escobar, E.A. y García Dávila, M.A. 2024. Comparison of maize performance between doubled haploids and different selfing generations in the Mexican subtropics. Acta Agronómica. 72, 2 (mar. 2024), 168–175. DOI:https://doi.org/10.15446/acag.v72n2.85349.

ACS

(1)
Vargas Escobar, E. A.; García Dávila, M. A. Comparison of maize performance between doubled haploids and different selfing generations in the Mexican subtropics. Acta Agron. 2024, 72, 168-175.

ABNT

VARGAS ESCOBAR, E. A.; GARCÍA DÁVILA, M. A. Comparison of maize performance between doubled haploids and different selfing generations in the Mexican subtropics. Acta Agronómica, [S. l.], v. 72, n. 2, p. 168–175, 2024. DOI: 10.15446/acag.v72n2.85349. Disponível em: https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/85349. Acesso em: 10 jul. 2024.

Chicago

Vargas Escobar, Ever Andrés, y Mario Augusto García Dávila. 2024. «Comparison of maize performance between doubled haploids and different selfing generations in the Mexican subtropics». Acta Agronómica 72 (2):168-75. https://doi.org/10.15446/acag.v72n2.85349.

Harvard

Vargas Escobar, E. A. y García Dávila, M. A. (2024) «Comparison of maize performance between doubled haploids and different selfing generations in the Mexican subtropics», Acta Agronómica, 72(2), pp. 168–175. doi: 10.15446/acag.v72n2.85349.

IEEE

[1]
E. A. Vargas Escobar y M. A. García Dávila, «Comparison of maize performance between doubled haploids and different selfing generations in the Mexican subtropics», Acta Agron., vol. 72, n.º 2, pp. 168–175, mar. 2024.

MLA

Vargas Escobar, E. A., y M. A. García Dávila. «Comparison of maize performance between doubled haploids and different selfing generations in the Mexican subtropics». Acta Agronómica, vol. 72, n.º 2, marzo de 2024, pp. 168-75, doi:10.15446/acag.v72n2.85349.

Turabian

Vargas Escobar, Ever Andrés, y Mario Augusto García Dávila. «Comparison of maize performance between doubled haploids and different selfing generations in the Mexican subtropics». Acta Agronómica 72, no. 2 (marzo 19, 2024): 168–175. Accedido julio 10, 2024. https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/85349.

Vancouver

1.
Vargas Escobar EA, García Dávila MA. Comparison of maize performance between doubled haploids and different selfing generations in the Mexican subtropics. Acta Agron. [Internet]. 19 de marzo de 2024 [citado 10 de julio de 2024];72(2):168-75. Disponible en: https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/85349

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