Inhibition and stimulation of mycelial growth of Moniliophthora roreri by flutolanil in populations of Ecuador

Darlyn José Amaya Márquez; Fernando Espinoza-Lozano; Mirian Elizabeth Villavicencio-Vásquez; Daynet Sosa del Castillo; Simón Pérez-Martínez

doi:10.15446/acag.v70n3.88905

Publicado

2022-03-25

Inhibition and stimulation of mycelial growth of Moniliophthora roreri by flutolanil in populations of Ecuador

Inhibición y estimulación del crecimiento micelial de Moniliophthora roreri por flutolanil en poblaciones de Ecuador

DOI:

https://doi.org/10.15446/acag.v70n3.88905

Palabras clave:

Sensitivity to fungicide, moniliasis, frosty pod rot. (en)
Sensibilidad a fungicidas, Moniliasis, Pudrición helada de la mazorca (es)

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Autores/as

Darlyn José Amaya Márquez Universidad Agraria del Ecuador https://orcid.org/0000-0003-4961-0283
Fernando Espinoza-Lozano Escuela Superior Politécnica del Litoral–ESPOL https://orcid.org/0000-0002-2051-2682
Mirian Elizabeth Villavicencio-Vásquez Escuela Superior Politécnica del Litoral–ESPOL https://orcid.org/0000-0003-3237-0295
Daynet Sosa del Castillo Escuela Superior Politécnica del Litoral–ESPOL https://orcid.org/0000-0001-5403-9072
Simón Pérez-Martínez Universidad Estatal de Milagro https://orcid.org/0000-0001-7349-7036

Resumen (en)
Resumen (es)

Frosty pod rot (FPR) of cacao is caused by Moniliophthora roreri (MR). Effective management must include chemical or biological control, in addition to agronomic tactics. Flutolanil has been effective in controlling FPR. The objective of this research was to determine the response to flutolanil of MR isolates from the Amazon and Coast regions of Ecuador. Percentage of mycelial growth inhibition (PGI), and medium inhibitory concentration (IC₅₀) against three concentrations of the fungicide were determined. One µg mL^-1 of flutolanil in the culture medium inhibited completely the growth of the 76 MR isolates. At 0.1 μg mL^-1, 74/76 were inhibited between 70-97 % compared to the control. Conversely, at the lower concentrations (0.01-0.001 μg mL^-1) the results of inhibition were only of 22 % and 47 %, respectively. Four groups were identified: one included the most sensitive (66/76) to different degrees, and three groups representing 10 isolates that were stimulated at low concentrations of flutolanil. IC₅₀ values were low, indicating very high sensitivity in the MR population. IC₅₀max were 0.1342 and 0.1457 in two isolates from the Coast and the Amazon regions, respectively. Isolates from the Coast were significantly less sensitive to flutolanil than those from the Amazon ( IC₅₀ = 0.046 ± 0.03 and IC₅₀ = 0.030 ± 0.02, respectively), however, the differences were minimal. There were no significant differences when comparing the provinces. The most stimulated isolates were found in the provinces of Orellana and Los Ríos. It is concluded that flutolanil seems effective against the causal agent of FPR in Ecuador, both Coast and Amazon regions.

La pudrición helada de la mazorca (PHM) del cacao es causada por Moniliophthora roreri (MR). El manejo efectivo debe incluir el control químico o biológico, además de las prácticas agronómicas. El flutolanil ha sido eficaz controlando la PHF. El objetivo de esta investigación fue determinar la respuesta al flutolanil de aislados de MR de las regiones Amazonas y Costa de Ecuador. Se determinaron el porcentaje de inhibición del crecimiento micelial (PGI) y la concentración inhibitoria media (IC₅₀) en respuesta a tres concentraciones del fungicida. A 1 µg mL^-1 de flutolanil en el medio de cultivo se inhibió totalmente el crecimiento de los 76 aislados de MR. A 0.1 μg mL^-1; 74/76 fueron inhibidas entre el 70-97 % en comparación con el control. Por el contrario, a las concentraciones más bajas (0.01-0.001 μg mL-¹) los resultados de inhibición fueron solo del 22 % y 47 %, respectivamente. Se identificaron cuatros grupos: uno incluyó a la mayoría sensible (66/76) en diferente medida y tres grupos representaron 10 aislamientos que fueron estimulados a bajas concentraciones de flutolanil. Los IC₅₀ fueron bajos, lo que indica una alta sensibilidad de la población de MR. Los IC₅₀max fueron 0.1342 y 0.1457 en dos aislamientos de la costa y el Amazonas, respectivamente. Los aislados de la costa fueron significativamente menos sensibles al flutolanil que los del Amazonas ( IC₅₀ = 0.046 ± 0.03 y IC₅₀ = 0.030 ± 0.02, respectivamente), sin embargo, las diferencias fueron mínimas. No hubo diferencias significativas al comparar las provincias. Los aislados más estimulados se encontraron en las provincias de Orellana y de Los Ríos. Se concluye que el flutolanil parece efectivo para el manejo del agente causal de la PHM en la Costa y en el Amazonas.

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APA

Amaya Márquez, D. J., Espinoza-Lozano, F., Villavicencio-Vásquez, M. E., Sosa del Castillo, D. y Pérez-Martínez, S. (2022). Inhibition and stimulation of mycelial growth of Moniliophthora roreri by flutolanil in populations of Ecuador. Acta Agronómica, 70(3). https://doi.org/10.15446/acag.v70n3.88905

ACM

[1]

Amaya Márquez, D.J., Espinoza-Lozano, F., Villavicencio-Vásquez, M.E., Sosa del Castillo, D. y Pérez-Martínez, S. 2022. Inhibition and stimulation of mycelial growth of Moniliophthora roreri by flutolanil in populations of Ecuador. Acta Agronómica. 70, 3 (mar. 2022). DOI:https://doi.org/10.15446/acag.v70n3.88905.

ACS

(1)

Amaya Márquez, D. J.; Espinoza-Lozano, F.; Villavicencio-Vásquez, M. E.; Sosa del Castillo, D.; Pérez-Martínez, S. Inhibition and stimulation of mycelial growth of Moniliophthora roreri by flutolanil in populations of Ecuador. Acta Agron. 2022, 70.

ABNT

AMAYA MÁRQUEZ, D. J.; ESPINOZA-LOZANO, F.; VILLAVICENCIO-VÁSQUEZ, M. E.; SOSA DEL CASTILLO, D.; PÉREZ-MARTÍNEZ, S. Inhibition and stimulation of mycelial growth of Moniliophthora roreri by flutolanil in populations of Ecuador. Acta Agronómica, [S. l.], v. 70, n. 3, 2022. DOI: 10.15446/acag.v70n3.88905. Disponível em: https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/88905. Acesso em: 23 jul. 2024.

Chicago

Amaya Márquez, Darlyn José, Fernando Espinoza-Lozano, Mirian Elizabeth Villavicencio-Vásquez, Daynet Sosa del Castillo, y Simón Pérez-Martínez. 2022. «Inhibition and stimulation of mycelial growth of Moniliophthora roreri by flutolanil in populations of Ecuador». Acta Agronómica 70 (3). https://doi.org/10.15446/acag.v70n3.88905.

Harvard

Amaya Márquez, D. J., Espinoza-Lozano, F., Villavicencio-Vásquez, M. E., Sosa del Castillo, D. y Pérez-Martínez, S. (2022) «Inhibition and stimulation of mycelial growth of Moniliophthora roreri by flutolanil in populations of Ecuador», Acta Agronómica, 70(3). doi: 10.15446/acag.v70n3.88905.

IEEE

[1]

D. J. Amaya Márquez, F. Espinoza-Lozano, M. E. Villavicencio-Vásquez, D. Sosa del Castillo, y S. Pérez-Martínez, «Inhibition and stimulation of mycelial growth of Moniliophthora roreri by flutolanil in populations of Ecuador», Acta Agron., vol. 70, n.º 3, mar. 2022.

MLA

Amaya Márquez, D. J., F. Espinoza-Lozano, M. E. Villavicencio-Vásquez, D. Sosa del Castillo, y S. Pérez-Martínez. «Inhibition and stimulation of mycelial growth of Moniliophthora roreri by flutolanil in populations of Ecuador». Acta Agronómica, vol. 70, n.º 3, marzo de 2022, doi:10.15446/acag.v70n3.88905.

Turabian

Amaya Márquez, Darlyn José, Fernando Espinoza-Lozano, Mirian Elizabeth Villavicencio-Vásquez, Daynet Sosa del Castillo, y Simón Pérez-Martínez. «Inhibition and stimulation of mycelial growth of Moniliophthora roreri by flutolanil in populations of Ecuador». Acta Agronómica 70, no. 3 (marzo 25, 2022). Accedido julio 23, 2024. https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/88905.

Vancouver

1.

Amaya Márquez DJ, Espinoza-Lozano F, Villavicencio-Vásquez ME, Sosa del Castillo D, Pérez-Martínez S. Inhibition and stimulation of mycelial growth of Moniliophthora roreri by flutolanil in populations of Ecuador. Acta Agron. [Internet]. 25 de marzo de 2022 [citado 23 de julio de 2024];70(3). Disponible en: https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/88905

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