Correo Electrónico
DNINFOA - SIA
Bibliotecas
Convocatorias
Identidad U.N.
Publicado
ACTIVIDAD ANTIFÚNGICA Y CARACTERÍSTICAS DE PROMOCIÓN DE CRECIMIENTO VEGETAL DE Pseudomonas aeruginosa y Enterobacter sp. DEGRADADORAS DE HIDROCARBUROS AISLADAS DE SUELO CONTAMINADO
Antifungal activity and plant growth promotion characteristics of hydrocarbon degrading Pseudomonas aeruginosa and Enterobacter sp. Isolated from polluted soil
DOI:
https://doi.org/10.15446/abc.v27n3.92758Palabras clave:
Bacterias, Diesel, Biodegradación, Actividad contra hongos, Tolerancia a metales (es)Bacteria, Diesel, Biodegradation, Antifungal activity, Metal tolerance (en)
Descargas
El diésel es una mezcla compleja de hidrocarburos alifáticos y aromáticos, que por su amplio uso se ha convertido en un contaminante ambiental muy frecuente. Debido a esto, es imperativo explorar alternativas viables y económicas para la remoción de dicho contaminante. El propósito del presente trabajo fue analizar la degradación de diésel por bacterias aisladas de suelo contaminado con esa mezcla de hidrocarburos, así como evaluar su actividad antagónica sobre hongos fitopatógenos, sus características de promoción del crecimiento vegetal y tolerancia a metales. A partir del enriquecimiento en diésel como única fuente de carbono, se obtuvieron los aislados bacterianos J3 y S3, cuya identificación bioquímica y molecular reveló que corresponden a Pseudomonas aeruginosa y Enterobacter sp., respectivamente. Además, se observó que el crecimiento bacteriano fue mejor entre 2 y 5 % de diésel, mientras que el pH óptimo fue de 7,0 y 8,0 en presencia de 3 % de diésel. También, S3 mostró buen crecimiento a concentraciones de hasta 4 % de NaCl. Por otro lado, las bacterias mostraron inhibición del crecimiento micelial de los hongos fitopatógenos Alternaria sp., Botrytis cinerea, Colletotrichum siamense y Fusarium proliferatum. Además de características de promoción de crecimiento vegetal como producción de ácido indol acético (AIA), solubilización de fosfato, producción de sideróforos y surfactantes. También, se observó que las bacterias crecieron en presencia de metales como Zn, Cu, Ba y Pb, en concentraciones de entre 1,5 y >10 mM. En conclusión, las bacterias aisladas e identificadas en este estudio presentan características que las hacen excelentes candidatas para la remoción de hidrocarburos solas o mediante fitorremediación por sus características de promoción de crecimiento vegetal.
Diesel oil is a complex mixture of aliphatic and aromatic hydrocarbons, which due to the wide usage has become a frequent environmental pollutant. Then, it is imperative to explore viable and economic alternatives for diesel oil degradation. The purpose of this work was to analyze the degradation of diesel oil by bacteria isolated from polluted soil, as well as to evaluate the antagonistic activity against phytopathogenic fungi, the plant growth promotion characteristics, and metal tolerance. From the enrichment in diesel oil as carbon source the J3 and S3 bacterial isolates were obtained, the biochemical and molecular identification indicates that these isolates were Pseudomonas aeruginosa and Enterobacter sp., respectively. Furthermore, the bacterial growth between 2 and 5 % of diesel oil was better, whereas the optimal pH was of 7.0 and 8.0 in the presence of 3 % of diesel oil, also, S3 showed growth at concentration of 4 % of NaCl. Even more, both bacteria showed inhibition of the phytopathogenic fungi Alternaria sp., Botrytis cinerea, Colletotrichum siamense and Fusarium proliferatum and plant growth promoting traits as indoleacetic acid (IAA) production, phosphate solubilization, siderophores and surfactant production. Also, bacterial growth was observed specially for Zn, Cu, Ba, and Pb, in concentrations between 1.5 and >10 mM. In conclusion, the bacteria identified in this study presented characteristics that made them good candidates for the remotion of hydrocarbons alone or by phytoremediation due to its haracteristics of plant growth promotion.
Referencias
Ahmad, S. A., Ku Ahamad, K. N. E., Wan Johari, W. L., Halmi, M. I. E., Shukor, M. Y., y Yusof, M. T. (2014). Kinetics of diesel degradation by an acrylamide degrading bacterium. Rendiconti Lincei, 25(4), 505–512. https://doi.org/10.1007/s12210-014-0344-7
Al-Hawash, A. B., Dragh, M. A., Li, S., Alhujaily, A., Abbood, H. A., Zhang, X., y Ma, F. (2018). Principles of microbial degradation of petroleum hydrocarbons in the environment. The Egyptian Journal of Aquatic Research, 44(2), 71–76. https://doi.org/10.1016/j.ejar.2018.06.001
Alshahri, F., y El-Taher, A. (2018). Assessment of Heavy and Trace Metals in Surface Soil Nearby an Oil Refinery, Saudi Arabia, Using Geoaccumulation and Pollution Indices. Archives of Environmental Contamination and Toxicology, 75(3), 390–401. https://doi.org/10.1007/s00244-018-0531-0
Arslan, M., Imran, A., Khan, Q. M., y Afzal, M. (2017). Plant–bacteria partnerships for the remediation of persistent organic pollutants. Environmental Science and Pollution Research, 24(5), 4322–4336. https://doi.org/10.1007/s11356-015-4935-3
Caballero-Flores, G. G., Acosta-Navarrete, Y. M., Ramírez-Díaz, M. I., Silva-Sánchez, J., y Cervantes, C. (2012). Chromate-resistance genes in plasmids from antibiotic-resistant nosocomial enterobacterial isolates. FEMS Microbiology Letters, 327(2), 148–154. https://doi.org/10.1111/j.1574-6968.2011.02473.x
Dahalan, S. F. A., Yunus, I., Johari, W. L. W., Shukor, M. Y., Halmi, M. I. E., Shamaan, N. A., y Syed, M. A. (2014). Growth kinetics of a diesel-degrading bacterial strain from petroleum-contaminated soil. Journal of Environmental Biology, 35(2), 399–406. http://www.ncbi.nlm.nih.gov/pubmed/24665769
Dos Santos, J. J., y Maranho, L. T. (2018). Rhizospheric microorganisms as a solution for the recovery of soils contaminated by petroleum: A review. Journal of Environmental Management, 210, 104–113. https://doi.org/10.1016/j.jenvman.2018.01.015
Feng, N.-X., Yu, J., Zhao, H.-M., Cheng, Y.-T., Mo, C.-H., Cai, Q.-Y., Li, Y.-W., Li, H., y Wong, M.-H. (2017). Efficient phytoremediation of organic contaminants in soils using plant–endophyte partnerships. Science of The Total Environment, 583, 352–368. https://doi.org/10.1016/j.scitotenv.2017.01.075
Garrido-Sanz, D., Redondo-Nieto, M., Guirado, M., Pindado Jiménez, O., Millán, R., Martin, M., y Rivilla, R. (2019). Metagenomic Insights into the Bacterial Functions of a Diesel-Degrading Consortium for the Rhizoremediation of Diesel-Polluted Soil. Genes, 10(6), 456. https://doi.org/10.3390/genes10060456
Hanafy, A. A.-E. M. EL, Anwar, Y., Mohamed, S. A., Al-Garni, S. M. S., Sabir, J. S. M., AbuZinadah, O. A., Mehdar, H. Al, Alfaidi, A. W., y Ahmed, M. M. M. (2016). Isolation and identification of bacterial consortia responsible for degrading oil spills from the coastal area of Yanbu, Saudi Arabia. Biotechnology & Biotechnological Equipment, 30(1), 69–74. https://doi.org/10.1080/13102818.2015.1086282
Hanson, K. G., Desai, J. D., y Desai, A. J. (1993). A rapid and simple screening technique for potential crude oil degrading microorganisms. Biotechnology Techniques, 7(10), 745–748. https://doi.org/10.1007/BF00152624
Hao, K., Ullah, H., Qin, X., Li, H., Li, F., y Guo, P. (2019). Effectiveness of Bacillus pumilus PDSLzg-1, an innovative Hydrocarbon-Degrading Bacterium conferring antifungal and plant growth-promoting function. 3 Biotech, 9(8), 305. https://doi.org/10.1007/s13205-019-1842-1
Hewelke, E., Szatyłowicz, J., Hewelke, P., Gnatowski, T., y Aghalarov, R. (2018). The Impact of Diesel Oil Pollution on the Hydrophobicity and CO2 Efflux of Forest Soils. Water, Air, & Soil Pollution, 229(2), 51. https://doi.org/10.1007/s11270-018-3720-6
Jiang, H., Dong, H., Zhang, G., Yu, B., Chapman, L. R., y Fields, M. W. (2006). Microbial Diversity in Water and Sediment of Lake Chaka, an Athalassohaline Lake in Northwestern China. Applied and Environmental Microbiology, 72(6), 3832–3845. https://doi.org/10.1128/AEM.02869-05
Lee, D. W., Lee, H., Kwon, B.-O., Khim, J. S., Yim, U. H., Kim, B. S., y Kim, J.-J. (2018). Biosurfactant-assisted bioremediation of crude oil by indigenous bacteria isolated from Taean beach sediment. Environmental Pollution, 241, 254–264. https://doi.org/10.1016/j.envpol.2018.05.070
Liu, J., Bacosa, H. P., y Liu, Z. (2017). Potential Environmental Factors Affecting Oil-Degrading Bacterial Populations in Deep and Surface Waters of the Northern Gulf of Mexico. Frontiers in Microbiology, 7. https://doi.org/10.3389/fmicb.2016.02131
Mariano, A. P., Bonotto, D. M., Angelis, D. de F. de, Pirôllo, M. P. S., y Contiero, J. (2008). Biodegradability of commercial and weathered diesel oils. Brazilian Journal of Microbiology, 39(1), 133–142. https://doi.org/10.1590/S1517-83822008000100028
Martínez-Absalón, S., Rojas-Solís, D., Hernández-León, R., Prieto-Barajas, C., Orozco-Mosqueda, M. del C., Peña-Cabriales, J. J., Sakuda, S., Valencia-Cantero, E., y Santoyo, G. (2014). Potential use and mode of action of the new strain Bacillus thuringiensis UM96 for the biological control of the grey mould phytopathogen Botrytis cinerea. Biocontrol Science and Technology, 24(12), 1349–1362. https://doi.org/10.1080/09583157.2014.940846
Menezes Bento, F., de Oliveira Camargo, F. A., Okeke, B. C., y Frankenberger, W. T. (2005). Diversity of biosurfactant producing microorganisms isolated from soils contaminated with diesel oil. Microbiological Research, 160(3), 249–255. https://doi.org/10.1016/j.micres.2004.08.005
Muthukamalam, S., Sivagangavathi, S., Dhrishya, D., y Sudha Rani, S. (2017). Characterization of dioxygenases and biosurfactants produced by crude oil degrading soil bacteria. Brazilian Journal of Microbiology, 48(4), 637–647. https://doi.org/10.1016/j.bjm.2017.02.007
Nwaichi, E. O., Wegwu, M. O., y Nwosu, U. L. (2014). Distribution of selected carcinogenic hydrocarbon and heavy metals in an oil-polluted agriculture zone. Environmental Monitoring and Assessment, 186(12), 8697–8706. https://doi.org/10.1007/s10661-014-4037-6
Obi, L. U., Atagana, H. I., y Adeleke, R. A. (2016). Isolation and characterisation of crude oil sludge degrading bacteria. SpringerPlus, 5(1), 1946. https://doi.org/10.1186/s40064-016-3617-z
Palanisamy, N., Ramya, J., Kumar, S., Vasanthi, N., Chandran, P., y Khan, S. (2014). Diesel biodegradation capacities of indigenous bacterial species isolated from diesel contaminated soil. Journal of Environmental Health Science and Engineering, 12(1), 142. https://doi.org/10.1186/s40201-014-0142-2
Pawlik, M., Cania, B., Thijs, S., Vangronsveld, J., y Piotrowska-Seget, Z. (2017). Hydrocarbon degradation potential and plant growth-promoting activity of culturable endophytic bacteria of Lotus corniculatus and Oenothera biennis from a long-term polluted site. Environmental Science and Pollution Research, 24(24), 19640–19652. https://doi.org/10.1007/s11356-017-9496-1
Peixoto, F. B. S., Peixoto, J. C. D. C., Motta, D. C. L., Peixoto, A. T. M., Pereira, J. O., y Astolfi-Filho, S. (2018). Assessment of petroleum biodegradation for Bacillus toyonensis by the using redox indicator 2,6 dichlorophenol indophenol. Acta Scientiarum. Biological Sciences, 40(1), 35640. https://doi.org/10.4025/actascibiolsci.v40i1.35640
Pereira, S. I. A., y Castro, P. M. L. (2014). Phosphate solubilizing rhizobacteria enhance Zea mays growth in agricultural P-deficient soils. Ecological Engineering, 73, 526–535. https://doi.org/10.1016/j.ecoleng.2014.09.060
Ramadass, K., Megharaj, M., Venkateswarlu, K., y Naidu, R. (2016). Soil bacterial strains with heavy metal resistance and high potential in degrading diesel oil and n-alkanes. International Journal of Environmental Science and Technology, 13(12), 2863–2874. https://doi.org/10.1007/s13762-016-1113-1
Ramasamy, S., Arumugam, A., y Chandran, P. (2017). Optimization of Enterobacter cloacae (KU923381) for diesel oil degradation using response surface methodology (RSM). Journal of Microbiology, 55(2), 104–111. https://doi.org/10.1007/s12275-017-6265-2
Schwyn, B., y Neilands, J. B. (1987). Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry, 160(1), 47–56. https://doi.org/10.1016/0003-2697(87)90612-9
Sheng, X.-F., y Xia, J.-J. (2006). Improvement of rape (Brassica napus) plant growth and cadmium uptake by cadmium resistant bacteria. Chemosphere, 64(6), 1036–1042. https://doi.org/10.1016/j.chemosphere.2006.01.051
Tamura, K., Stecher, G., Peterson, D., Filipski, A., y Kumar, S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution, 30(12), 2725–2729. https://doi.org/10.1093/molbev/mst197
Varjani, S. J. (2017). Microbial degradation of petroleum hydrocarbons. Bioresource Technology, 223, 277–286. https://doi.org/10.1016/j.biortech.2016.10.037
Wilson, K. (2001). Preparation of Genomic DNA from Bacteria. Current Protocols in Molecular Biology, 56(1). https://doi.org/10.1002/0471142727.mb0204s56
Wu, T., Xu, J., Liu, J., Guo, W.-H., Li, X.-B., Xia, J.-B., Xie, W.-J., Yao, Z.-G., Zhang, Y.-M., y Wang, R.-Q. (2019). Characterization and Initial Application of Endophytic Bacillus safensis Strain ZY16 for Improving Phytoremediation of Oil-Contaminated Saline Soils. Frontiers in Microbiology, 10. https://doi.org/10.3389/fmicb.2019.00991
Wu, T., Xu, J., Xie, W., Yao, Z., Yang, H., Sun, C., y Li, X. (2018). Pseudomonas aeruginosa L10: A Hydrocarbon-Degrading, Biosurfactant-Producing, and Plant-Growth-Promoting Endophytic Bacterium Isolated From a Reed (Phragmites australis). Frontiers in Microbiology, 9. https://doi.org/10.3389/fmicb.2018.01087
Xu, X., Liu, W., Tian, S., Wang, W., Qi, Q., Jiang, P., Gao, X., Li, F., Li, H., y Yu, H. (2018). Petroleum Hydrocarbon-Degrading Bacteria for the Remediation of Oil Pollution Under Aerobic Conditions: A Perspective Analysis. Frontiers in Microbiology, 9. https://doi.org/10.3389/fmicb.2018.02885
Zanaroli, G., Di Toro, S., Todaro, D., Varese, G. C., Bertolotto, A., y Fava, F. (2010). Characterization of two diesel fuel degrading microbial consortia enriched from a non acclimated, complex source of microorganisms. Microbial Cell Factories, 9(1), 10. https://doi.org/10.1186/1475-2859-9-10
Cómo citar
APA
ACM
ACS
ABNT
Chicago
Harvard
IEEE
MLA
Turabian
Vancouver
Descargar cita
Licencia
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-CompartirIgual 4.0.
1. La aceptación de manuscritos por parte de la revista implicará, además de su edición electrónica de acceso abierto bajo licencia Attribution-NonCommercial-ShareAlike 4.0 (CC BY NC SA), la inclusión y difusión del texto completo a través del repositorio institucional de la Universidad Nacional de Colombia y en todas aquellas bases de datos especializadas que el editor considere adecuadas para su indización con miras a incrementar la visibilidad de la revista.
2. Acta Biológica Colombiana permite a los autores archivar, descargar y compartir, la versión final publicada, así como las versiones pre-print y post-print incluyendo un encabezado con la referencia bibliográfica del articulo publicado.
3. Los autores/as podrán adoptar otros acuerdos de licencia no exclusiva de distribución de la versión de la obra publicada (p. ej.: depositarla en un archivo telemático institucional o publicarla en un volumen monográfico) siempre que se indique la publicación inicial en esta revista.
4. Se permite y recomienda a los autores/as difundir su obra a través de Internet (p. ej.: en archivos institucionales, en su página web o en redes sociales cientificas como Academia, Researchgate; Mendelay) lo cual puede producir intercambios interesantes y aumentar las citas de la obra publicada. (Véase El efecto del acceso abierto).
