Publicado

2016-01-01

Evaluación de tres promotores constitutivos para la expresión GUS en arroz (Oryza sativa L., cv. J-104)

Assessment three constitutive promoters for GUS expression in rice (Oryza sativa L., var. J-104)

Palabras clave:

CaMV 35S, chimerical A9 promoter, ubiquitine-1 (es)

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Autores/as

  • Maylin Pérez Bernal Center for Genetic Engineering and Biotechnology of Sancti Spiritus. P.O.Box 83, P.C. 60200. Sancti Spiritus, Cuba
  • Daymí Abreu Remedios Research Department. Center for Genetic Engineering and Biotechnology of Sancti Spiritus
  • Onel Valdivia Pérez Research Department. Center for Genetic Engineering and Biotechnology of Sancti Spiritus
  • Magalis Delgado Rigo Research Department. Center for Genetic Engineering and Biotechnology of Sancti Spiritus
  • Raúl Armas Ramos Research Department. Center for Genetic Engineering and Biotechnology of Sancti Spiritus

Se analizó la expresión constitutiva del gen reportero de la ß-Glucuronidasa (GUS) fusionado a tres promotores: el 35S del virus del mosaico de la coliflor (CaMV), el promotor quimérico A9 que contiene la actina-1 de arroz y el promotor ubiquitina-1 de maíz. La actividad de los promotores fue analizada cualitativa y cuantitativamente en diferentes tejidos y estadíos de crecimiento de plantas de arroz (variedad J-104) transformadas mediante biobalística. Se demostró la expresión constitutiva de GUS bajo los promotores estudiados, con distintos patrones de actividad relativa en hojas, tallos y raíces de plantas in vitro y ex vitro, y en plantas de la progenie T1. Bajo el promotor quimérico A9 se lograron los mayores niveles de expresión GUS en todos los tejidos y fases de crecimiento de las plantas.

This work analyzed the constitutive expression of the ß-Glucuronidase (GUS) reporter gene fused to three promoters: the cauliflower mosaic virus (CaMV) 35S, the chimerical A9 promoter which contains rice Act1, and the Ubiquitine-1 promoter from maize. The activity of the promoters was qualitative and quantitatively obtained in different tissues and various growth stages of rice plants (cv J-104) transformed by biolistic. All the promoters were found to be active, with distinct patterns of relative activity in leaves, stem and roots from in vitro and ex vitro plants, and in plants of T1 progeny. The chimerical A9 promoter increased significantly levels of GUS expression in all the tissues and at all growth stages of the plants.

Key words: CaMV 35S, chimerical A9 promoter, ubiquitine-1.

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Citas

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Derechos de autor 2016 Revista Colombiana de Biotecnología

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