Publicado

2018-07-01

Factores que afectan la expresión transitoria del gen GUS en yuca (Manihot esculenta Crantz)

Factors affecting the transient expression of gene GUS in cassava (Manihot esculenta Crantz)

Fatores que afetam a expressão transitória do gene GUS na mandioca (Manihot esculenta Crantz)

DOI:

https://doi.org/10.15446/rev.colomb.biote.v20n2.77063

Palabras clave:

Agrobacterium tumefaciens, Beta-glucuronidasa, VirG, agroinfiltración, yuca (es)
Agrobacterium tumefaciens, beta-glucuronidase, VirG, agroinfiltration, cassava (en)
Agrobacterium tumefaciens, Beta-glucuronidase, VirG, agroinfiltração, mandioca (pt)

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Autores/as

  • Edilene Ramírez M.SC., Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Biología, Grupo Manihot Biotec Calle 30 N 45-03, Bogota, Colombia. eramirezv@unal.edu.co
  • Boris Szurek Ph.D., Résistance des Plantes aux Bioagresseurs (RPB). Institut de Recherche pour le Développement. 911, Av. Agropolis BP 64501. Montpellier, Francia. boris.szurek@ird.fr
  • Camilo Ernesto Lopez Carrascal Ph.D., Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Biología, Grupo Manihot Biotec Calle 30 N 45-03, Bogota, Colombia. Correspondencia: celopezc@unal.edu.co

La expresión transitoria es una métodología ampliamente utilizada para el estudio de genes. Sin embargo, hasta la fecha no existe un reporte en donde se utilice esta técnica en hojas de yuca de plantas adultas. Por esta razón este trabajo se centró en la determinación de algunos parámetros críticos para la expresión transitoria del gen GUS en yuca como son: la metodología para introducir la bacteria, la cepa de Agrobacterium, el tiempo post-inoculación, la introducción del gen VirG y la expresión del gen GUS en algunas variedades de yuca. Los resultados indicaron niveles más altos de expresión del gen GUS entre 5-7 días post-inoculación (dpi), agroinfiltrando con la cepa GV3101 y un incremento en la virulencia de esta cepa mediante la introducción del gen VirG. Por último se observaron diferentes niveles de expresión del gen GUS entre las variedades de yuca evaluadas, lo que indica que el factor genético es clave en la eficiencia de la agroinfiltración en este cultivo.

Transient expression is a common technique used for the co-expression of proteins for a wide range of experiments in molecular biology. This technique has been frequently used for gene study in plant pathogen interaction and has proved to be versatile and effective. However, there are still few reports and sparse data for transient expression in cassava leaves from adult/mature plants. For this reason, in this study we evaluated some regards of transient expression for the gene GUS in cassava: bacteria introduction methods, type of Agrobacterium strains, time post-inoculation, introduction of gene VirG and GUS and their expression into different cassava varieties. The results show that GUS expression is more efficient between 5-7 days post-inoculation with the GV3101 strain, moreover the virulence of this strain increases with the VirG gene. Finally, we observed a range of GUS expression pattern between different cassava varieties, altogether showing that the genetic factor is important for an accurate and effective agroinfiltration in cassava.

A expressão transitória é uma metodologia amplamente utilizada para o estudo de genes. Entretanto, até o momento não há relato onde esta técnica é usada em folhas de mandioca de plantas adultas. Por esta razão, este trabalho focou-se na identificação de alguns parâmetros críticos para a expressão transiente do gene GUS em mandioca como são os métodos para a introdução da bactéria Agrobacterium estirpe, o tempo de pós-inoculação, a introdução do gene virG e a expressão do gene GUS em algumas variedades de mandioca. Os resultados indicam níveis mais elevados de expressão do gene GUS entre pós-inoculação (dpi), com agroinfiltrando GV3101 estirpe e aumentou de virulência desta estirpe por introdução do gene VirG 5-7 dias. Finalmente, diferentes níveis de expressão do gene GUS foram observados entre as variedades de mandioca avaliadas, o que indica que o fator genético é fundamental na eficiência da agroinfiltração nessa cultura.

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Cómo citar

APA

Ramírez, E., Szurek, B. y Lopez Carrascal, C. E. (2018). Factores que afectan la expresión transitoria del gen GUS en yuca (Manihot esculenta Crantz). Revista Colombiana de Biotecnología, 20(2), 57–67. https://doi.org/10.15446/rev.colomb.biote.v20n2.77063

ACM

[1]
Ramírez, E., Szurek, B. y Lopez Carrascal, C.E. 2018. Factores que afectan la expresión transitoria del gen GUS en yuca (Manihot esculenta Crantz). Revista Colombiana de Biotecnología. 20, 2 (jul. 2018), 57–67. DOI:https://doi.org/10.15446/rev.colomb.biote.v20n2.77063.

ACS

(1)
Ramírez, E.; Szurek, B.; Lopez Carrascal, C. E. Factores que afectan la expresión transitoria del gen GUS en yuca (Manihot esculenta Crantz). Rev. colomb. biotecnol. 2018, 20, 57-67.

ABNT

RAMÍREZ, E.; SZUREK, B.; LOPEZ CARRASCAL, C. E. Factores que afectan la expresión transitoria del gen GUS en yuca (Manihot esculenta Crantz). Revista Colombiana de Biotecnología, [S. l.], v. 20, n. 2, p. 57–67, 2018. DOI: 10.15446/rev.colomb.biote.v20n2.77063. Disponível em: https://revistas.unal.edu.co/index.php/biotecnologia/article/view/77063. Acesso em: 18 jul. 2024.

Chicago

Ramírez, Edilene, Boris Szurek, y Camilo Ernesto Lopez Carrascal. 2018. «Factores que afectan la expresión transitoria del gen GUS en yuca (Manihot esculenta Crantz)». Revista Colombiana De Biotecnología 20 (2):57-67. https://doi.org/10.15446/rev.colomb.biote.v20n2.77063.

Harvard

Ramírez, E., Szurek, B. y Lopez Carrascal, C. E. (2018) «Factores que afectan la expresión transitoria del gen GUS en yuca (Manihot esculenta Crantz)», Revista Colombiana de Biotecnología, 20(2), pp. 57–67. doi: 10.15446/rev.colomb.biote.v20n2.77063.

IEEE

[1]
E. Ramírez, B. Szurek, y C. E. Lopez Carrascal, «Factores que afectan la expresión transitoria del gen GUS en yuca (Manihot esculenta Crantz)», Rev. colomb. biotecnol., vol. 20, n.º 2, pp. 57–67, jul. 2018.

MLA

Ramírez, E., B. Szurek, y C. E. Lopez Carrascal. «Factores que afectan la expresión transitoria del gen GUS en yuca (Manihot esculenta Crantz)». Revista Colombiana de Biotecnología, vol. 20, n.º 2, julio de 2018, pp. 57-67, doi:10.15446/rev.colomb.biote.v20n2.77063.

Turabian

Ramírez, Edilene, Boris Szurek, y Camilo Ernesto Lopez Carrascal. «Factores que afectan la expresión transitoria del gen GUS en yuca (Manihot esculenta Crantz)». Revista Colombiana de Biotecnología 20, no. 2 (julio 1, 2018): 57–67. Accedido julio 18, 2024. https://revistas.unal.edu.co/index.php/biotecnologia/article/view/77063.

Vancouver

1.
Ramírez E, Szurek B, Lopez Carrascal CE. Factores que afectan la expresión transitoria del gen GUS en yuca (Manihot esculenta Crantz). Rev. colomb. biotecnol. [Internet]. 1 de julio de 2018 [citado 18 de julio de 2024];20(2):57-6. Disponible en: https://revistas.unal.edu.co/index.php/biotecnologia/article/view/77063

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