Evaluation of the fermentation process and final quality of five cacao clones from the department of Huila, Colombia.
Evaluación de la fermentación y calidad final de cinco clones de cacao proveniente del departamento del Huila, Colombia.
DOI:
https://doi.org/10.15446/dyna.v86n210.75814Palabras clave:
Cutting test Microfermentation, Polyphenols Quality, Sensory profile (en)Prueba de corte microfermentacion, Polifenoles, prueba sensorial. (es)
Descargas
The aim of this work was to study the microfermentation process of five cacao clones, ICS 01, ICS 95, ICS 39, TSH 565 and CCN 51, from the department of Huila, Colombia. These clones were characterized by their physicochemical properties followed by their fermentation process. The behavior of temperature, pH, total acidity, total polyphenols and fermentation index was recorded. Additionally, the physical cutting test was carried out at the end of the process, as well as the sensory evaluation of the cocoa liquor, in order to establish the sensory profile of each material. Results showed that fermentation time for clones ICS 95, ICS 01 was five days t was obtaining a standard cocoa quality for ICS 01. The other three clones ICS 39, TSH 565 and CCN 51 required six days of fermentation but a premium cocoa was obtained. The establishment of fermentation parameters allows improving final cocoa quality, and thus, increasing its commercial value.
En el presente trabajo se estudió la microfermentación de cinco clones de cacao, ICS 01, ICS 95, ICS 39, TSH 565 y CCN 51, provenientes del departamento del Huila, Colombia. Estos clones se caracterizaron por sus propiedades fisicoquímicas seguidos por su proceso de fermentación. Durante el proceso de microfermentación, se registró el comportamiento de la temperatura, pH, acidez total, polifenoles totales e índice de fermentación. Adicionalmente, se realizó la prueba física de corte al final del proceso y la evaluación sensorial del licor de cacao, con el fin de determinar un perfil sensorial de cada material. Los resultados mostraron que los tiempos de fermentación fueron de cinco días para los clones ICS 95 y ICS 01, obteniendo para este último un cacao tipo corriente. Los otros clones ICS 39, TSH 565 and CCN 51, requirieron seis días de fermentación, pero el cacao alcanzado fue tipo premio. La definición de parámetros de fermentación permite mejorar la calidad final de cacao y así aumentar su valor comercial.
Referencias
Rojas, F. and Sacristan, E. Environmental guide for the cultivation of cocoa. Ministry of Agriculture and Rural Development, National Cocoa Federation. Bogotá D.C. 2013, (2nd ed.)
Chocolate, Biscuits & Confectionery of Europe (CAOBISCO). European Cocoa Association, (ECA), and Federation of Cocoa Commerce (FCC). Cocoa in Grain: Quality Requirements for the Chocolate and Cocoa Industry. [Online], M. J. End & R. Dand, Eds, Europe. September, 2015. Available at: http://www.cocoaquality.eu/data/Cacao%20en%20Grano%20Requisitos%20de%20Calidad%20de%20la%20Industria%20Apr%202016_es.pdf
Gutiérrez, M. Manual of Quality Control Practices of Cocoa in Collection Center. Regional government Piura, APPROCAP, GTZ. Piura, Peru 2007Biehl, B.; Heinrichs, J.; Voigt, G.; Bytof, G. and Serrano, P. Nature of proteases and their action on storage proteins in cocoa seeds during germination as compared with fermentation. Proceedings of the 12th Cocoa Research Conference, Salvador, 1996.
Biehl, B.; Heinrichs, J.; Voigt, G.; Bytof, G. y Serrano, P. Nature of proteases and their action on storage proteins in cocoa seeds during germination as compared with fermentation. Proceedings of the 12th Cocoa Research Conference, Salvador, 1996.
Portillo, E., Labarca, M., Grazziani, L., Cros, E., Assemat, S., Davrieux, F. and Marcano, M. Formation of the aroma of Criollo cocoa (Theobroma cacao L.) in postharvest treatment function in Venezuela. Revista Científica UDO Agricola, [online] 9 (2), 458-468, 2009. [Date of reference July 22, 2017]. Available at: https://dialnet.unirioja.es/servlet/articulo?codigo=3308653
Sánchez Vargas, A., Castellanos Domínguez, Ó., and Domínguez Martinez, K. Road mapping for improving cocoa postharvest management. Engineering and Research, [Online] 28 (3), 150-158, 2008. [Date of reference August 22, 2017]. Available at: https://revistas.unal.edu.co/index.php/ingeinv/article/view/15135
García-Cáceres, R., Perdomo, A., Perdomo, A., Ortiz, O., Ortiz, O., Beltrán, P., Beltrán, P., Lopez, K., and Lopez, K. Characterization of the supply and value chains of Colombian cocoa. DYNA, 81 (187), 30-40, 2014. https://doi.org/10.15446/dyna.v81n187.39555
FEDECACAO. In 2012, Cocoa production increased 12% and the price fell 24%. Colombia Cacaotera 6 (15), 6-8, 2013.
Pinzon, J. O., and Rojas, O. Technical Guide for the Cultivation of Cocoa. Bogotá D.C, 2009.
Flórez M., D. H., Morales, A., Uribe G., C. P., & Contreras P., C. A. (2013). Analysis of trends in basic research for agroindustrial production chains. Corpoica Ciencia y Tecnología Agropecuaria, 13(2),121-135. https://doi.org/10.21930/rcta.vol13_num2_art:247
Philips, W., Arciniegas, A. and Mata Quirós, A. Catalog of cocoa clones. Costa Rica: Agronomic Center for Research and Teaching (CATIE), 2012.
ICONTEC. Colombian Institute of Technical Standards and Certification. Cocoa beans NTC 1252, Bogotá, D.C., 2013, 3rd ed., P12.
AGROSAVIA - The Colombian Corporation for Agricultural Research. Internal Cocoa Evaluation Protocols. 2015
ICONTE Colombian Institute of Technical Standards and Certification. Sensory Analysis, Methodology. Taste profile methods. NTC 3929, Bogotá, D.C., 1st Ed, 2014.
Rivera, R., Mecías Gallo, M., Guzmán Cedeño, G., Mercedes Peña, M., Medina Quinteros, H., Casanova Ferrín, M., Barrera Álvarez, A. and Nivela Morante, P. Effect of the type and time of fermentation in the physical and chemical quality of cocoa (Theobroma cacao L.) Science and Technology, [Online] 5 (1), 7-12, 2012 [date of reference July 28, 2017]. Available at:https://dialnet.unirioja.es/servlet/articulo?codigo=4149700
Amores, F.; Palacios, A.; Jiménez, J. and Zhang, D. Environmental environment, genetics, attributes of quality and singularization of Cacao in the northeast of the province of Esmeralda. Technical Bulletin.2009.
Recalde, S. Evaluation of the effect of pre-drying and fermentation time, on the contents of total polyphenols, alkaloids and volatile acids in two cocoa genotypes. PhD. Thesis Faculty of Chemical Sciences, Central University of Ecuador. Ecuador, 2007.
Hasing, M. Study of the variation in the contents of polyphenols and alkaloids, in cocoa almonds by the effect of the fermentation and roasting processes. PhD Thesis, Faculty of Sciences, School of Biochemistry and Pharmacy, Polytechnic School of Chimborazo. Riobamba, Ecuador, 2004.
Bertazzo, A., Comai, S., Brunato, I., Zancato, M. and Costa, C. The content of protein and non-protein (free and protein-bound) tryptophan in Theobroma cacao beans. FOOD chemistry, 124 (1), 93-96, 2011.https://doi.org/10.1016/j.foodchem.2010.05.110
Portillo, E.; Labarca, M.; Grazziani, L.; Cros, E., Assemat,S.; Davrieux, F. and Marcano, M. Formation of the aroma of Criollo cocoa (Theobroma cacao L.) as a function of post-harvest treatment in Venezuela. Revista Científica UDO Agrícola, [Online] 9 (2), 458-468, 2009. [Date of reference August 2, 2017]. Available at: http://www.bioline.org.br/pdf?cg09058
Fedecacao. Physicochemical characterization and benefit of cocoa bean (Theobroma cacao L.) National cocoa federation. Bogotá D.C, 2005.
Ulrich K., Ronald F., Hubert K., Böle B., Johannes W. and Jürgen V. Subunit structure of the vicilin-like globular storage protein of cocoa sedes and the origin of cocoa- and chocolate-specific aroma precursors. Food Chemistry 113, 903–913, 2009. https://doi.org/10.1016/j.foodchem.2008.08.017
Afoakwa, E. Chocolate Science and Technology. (1st ed.) Ghana: Wiley-Blackwell, 2010.
Cubillos, G., Merizalde, G. and Correa, E. Cocoa benefit manual 2008 for: technicians, professionals of the agricultural sector and producers. Medellin, Colombia, 2008.
López, A. Factors associated with cacao bean acidity and the possibility of its reduction by improved fermentation. Revista Theobroma, 13(3), p 233-248, 1983.
Portillo, E. Influence of fermentation on the quality of porcelain Creole cocoa (Theobroma cacao L.) in the South of Lake Maracaibo. Master's Thesis, Central University of Venezuela, Maracay - Venezuela, 2000.
Beckett, S. Industrial chocolate manufacture and use. (4th ed.) UK: Blackwell Publishing, 2009.
Espin, S. and Samaniego, I. Quality chemical parameters in almonds and cocoa liquor. Department of Nutrition and Quality, INIAP, 2006.
Zapata, S., Tamayo, A. and Rojano, BA. Effect of fermentation on the antioxidant activity of different Colombian cocoa clones. Cuban Journal Plant Med, [Online] vol.18, no.3, 91-404. Medellín, Colombia, 2013. [Date of reference August 5, 2017]. Available at: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962013000300007
Biehl, B., Meyer, B., Said, M.B., Samarakoddy, R.J., 1990. Bean spreading: a method of pulp preconditioning to impair strong nib acidification during cocoa fermentation in Malaysian. J. Food Agric. 51, 35–45.
Aprotosoaie A., Miron A., Trifan A., Simon V. and Costache I. 20016. The Cardiovascular Effects of Cocoa Polyphenols—An Overview. Diseases: 4, 39; doi:10.3390/diseases4040039
Ortiz de Bertorelli, L., Graziani de Fariñas, L. and Rovedas, G. Influence of several factors on characteristics of fermented cocoa beans and sun-dried. Tropical Agronomy, [Online]. vol.59, n.2 2009, [Date of reference October 31, 2017], pp. 119-127. Available at: http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0002-192X2009000200001
González Muñoz, Y., Pérez Sira, E. and Palomino Camargo, C. Factors that affect the sensory quality of chocolate. Nutrition, Vol.13 (4), 314-331, 2012.
Jinap, S., and Dimick, P. S. Acidic characteristics of fermented and dried cocoabeans from different countries of origin. Journal of Food Science, 55, 547-551, 1990. https://doi.org/10.1111/j.1365-2621.1990.tb06806.x
Mermet, G. Cacao: influence of roasting parameters on the consumption of aroma precursors and the formation of some volatile compounds. PhD, Thesis, Montpellier II University, Montpellier, France, 1989.
Cros, E., Jeanjean, N. and Tontillon, J. Formation of the cocoa aroma. Cocoa and chocolate production, use characteristics. Collection of science and agro-food techniques. [Online] 187-206, 1997. [Date of reference to September 22nd of 2017]. Available at: http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=15722
Jinap, S., Rosli, W. I. W., Russly, A. R., and Nordin, L. M. Effect of roasting time and temperature on volatile component profiles during nib roasting of cocoa beans (Theobroma cacao). Journal of the Science of Food and Agriculture, 77(4), 441-448, 1198. https://doi.org/10.1002/(SICI)1097-0010(199808)77:4<441::AID-JSFA46>3.0.CO;2-#
Cómo citar
IEEE
ACM
ACS
APA
ABNT
Chicago
Harvard
MLA
Turabian
Vancouver
Descargar cita
CrossRef Cited-by
1. V. Chaudhary, G. Mongia, MaryAnne Drake. (2025). Approaches to Determine the Flavor and Flavor Chemistry of Cocoa Beans and Cocoa Liquor. Journal of Food Science, 90(9) https://doi.org/10.1111/1750-3841.70481.
2. Camila R. Vizcaino‐Almeida, Daniel Guajardo‐Flores, Rodrigo Caroca‐Cáceres, Sergio O. Serna‐Saldívar, Miriam Briones‐García, Marco A. Lazo‐Vélez. (2022). Non‐conventional fermentation at laboratory scale of cocoa beans: Using probiotic microorganisms and substitution of mucilage by fruit pulps. International Journal of Food Science & Technology, 57(7), p.4307. https://doi.org/10.1111/ijfs.15757.
3. Edwin O. Baldeón, Lizbeth R. Mamani-Olgado, Bernardo O. Yépez Silva-Santisteban, Gabriela C. Chire-Fajardo, Nubia C. Martínez-Guerrero, Milber O. Ureña-Peralta. (2025). Impact of hot-air and infrared-assisted solar drying on the drying characteristics and physicochemical properties of cacao beans (Theobroma cacao L.). Frontiers in Sustainable Food Systems, 9 https://doi.org/10.3389/fsufs.2025.1596519.
4. César R. Balcázar-Zumaeta, Alexa J. Pajuelo-Muñoz, Deisy F. Trigoso-Rojas, Angel F. Iliquin-Chavez, Editha Fernández-Romero, Ives Yoplac, Lucas D. Muñoz-Astecker, Nadia Rodríguez-Hamamura, Ily M. Maza Mejía, Ilse S. Cayo-Colca, Gilson C. A. Chagas-Junior, Jorge L. Maicelo-Quintana, Efrain M. Castro-Alayo. (2023). Reduction in the Cocoa Spontaneous and Starter Culture Fermentation Time Based on the Antioxidant Profile Characterization. Foods, 12(17), p.3291. https://doi.org/10.3390/foods12173291.
5. Indah Anita Sari, Rudi Hari Murti, Misnawi, Eka Tarwaca Susila Putra, Bayu Setyawan, Miftahur Rizqi Akbar, Tri Rini Nuringtyas, Agung Wahyu Susilo. (2025). The differences non-volatile compound and flavor attributes in several Indonesian cocoa genotypes using medium scale fermentation. Beverage Plant Research, 5(1) https://doi.org/10.48130/bpr-0024-0041.
6. Desiana Nuriza Putri, Hans De Steur, Joel G. Juvinal, Xavier Gellynck, Joachim J. Schouteten. (2024). Sensory attributes of fine flavor cocoa beans and chocolate: A systematic literature review. Journal of Food Science, 89(4), p.1917. https://doi.org/10.1111/1750-3841.17006.
7. Eman Abbas Abduljawad. (2024). Exploring the Potential Advantages of Chocolate and Cocoa for Cardio and Cerebrovascular Health. Current Research in Nutrition and Food Science Journal, 12(3), p.1000. https://doi.org/10.12944/CRNFSJ.12.3.3.
8. Estefania García Gonzalez, John H. Mendez Orejuela, Jhon S. Sierra Banguera, Daniela I. Chamorro Moreno, Ginna A. Ordoñez Narváez, Andrés F. Ochoa Muñoz, Constanza Montalvo Rodriguez. (2022). Ecology and population dynamics of yeast starter cultures in cocoa beans fermentation. BioTechnologia, 103(4), p.343. https://doi.org/10.5114/bta.2022.120704.
9. Jenifer Criollo Nuñez, Cristina Ramirez‐Toro, German Bolivar, Angélica Piedad Sandoval A., María D. Lozano Tovar. (2023). Effect of microencapsulated inoculum of Pichia kudriavzevii on the fermentation and sensory quality of cacao CCN51 genotype. Journal of the Science of Food and Agriculture, 103(5), p.2425. https://doi.org/10.1002/jsfa.12433.
10. David Condori, Fabio Espichan, Ana Lucy Siccha Macassi, Luz Carbajal, Rosario Rojas. (2024). Study of the post-harvest processes of the peruvian chuncho cocoa using multivariate and multi-block analysis. Food Chemistry, 431, p.137123. https://doi.org/10.1016/j.foodchem.2023.137123.
11. María Alicia Peña González, Jennifer Pamela Ortiz Urgiles, Fernanda Alegría Santander Pérez, Marco Antonio Lazo Vélez, Rodrigo Sebastián Caroca Cáceres. (2023). Physicochemical changes during controlled laboratory fermentation of cocoa (CCN-51) with the inclusion of fruits and on-farm inoculation. Brazilian Journal of Food Technology, 26 https://doi.org/10.1590/1981-6723.01323.
12. Franco Lucio Ruiz-Santiago, Facundo Joaquín Márquez-Rocha, Pedro García-Alamilla, Areli Carrera-Lanestosa, Carolina Ramírez-López, Erik Ocaranza-Sánchez, David Jesús Jiménez-Rodríguez. (2024). Physicochemical and Biochemical Changes in Cocoa during the Fermentation Step. Fermentation, 10(8), p.405. https://doi.org/10.3390/fermentation10080405.
13. Myriam Rojas, Arne Hommes, Hero Jan Heeres, Farid Chejne. (2022). Physicochemical Phenomena in the Roasting of Cocoa (Theobroma cacao L.). Food Engineering Reviews, 14(3), p.509. https://doi.org/10.1007/s12393-021-09301-z.
14. E. L. Grimaldo-Pretel, M. A. Guerrero-Navarro, C.R. Vidal-Tovar, Y. Gordon-Hernández, P. J. Fragoso-Castilla. (2022). Incident factors in the quality of cocoa almonds obtained in small agricultural production units. IOP Conference Series: Materials Science and Engineering, 1253(1), p.012003. https://doi.org/10.1088/1757-899X/1253/1/012003.
Dimensions
PlumX
Visitas a la página del resumen del artículo
Descargas
Licencia
Derechos de autor 2019 DYNA

Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-SinDerivadas 4.0.
El autor o autores de un artículo aceptado para publicación en cualquiera de las revistas editadas por la facultad de Minas cederán la totalidad de los derechos patrimoniales a la Universidad Nacional de Colombia de manera gratuita, dentro de los cuáles se incluyen: el derecho a editar, publicar, reproducir y distribuir tanto en medios impresos como digitales, además de incluir en artículo en índices internacionales y/o bases de datos, de igual manera, se faculta a la editorial para utilizar las imágenes, tablas y/o cualquier material gráfico presentado en el artículo para el diseño de carátulas o posters de la misma revista.




