Publicado

2023-03-23

Evaluation of the antifungal activity and mode of action of citral against Cladophialophora carrionii.

Evaluación de la actividad antifúngica y forma de acción de citral frente a Cladophialophora carrionii.

Avaliação da atividade antifúngica e modo de ação do citral contra Cladophialophora carrionii.

DOI:

https://doi.org/10.15446/rcciquifa.v51n3.100452

Palabras clave:

Antifungal, Chromoblastomycosis, Citral, Cladophialophora carrionii, Monoterpene (en)
Antifúngico, chromoblastomicosis, citral, Cladophialophora carrionii, monoterpeno (es)
Antifúngico, cromoblastomicose, citral, Cladophialophora carrionii, monoterpeno (pt)

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Autores/as

  • Camilla de Menezes Department of Pharmaceutical Sciences (DCF), Center of Health Sciences (CCS), Federal University of Paraíba (UFPB). Castelo Branco III, João Pessoa-PB.
  • Ana Perez Department of Pharmaceutical Sciences (DCF), Center of Health Sciences (CCS), Federal University of Paraíba (UFPB). Castelo Branco III, João Pessoa-PB.
  • Janiere Sousa Department of Pharmaceutical Sciences (DCF), Center of Health Sciences (CCS), Federal University of Paraíba (UFPB). Castelo Branco III, João Pessoa-PB.
  • Katharina Ramos Departament of Pharmaceutical Sciences, State University of Paraíba (UEPB). Campus Universitário, Bodocongó, Campina Grande-PB.
  • Hermes Diniz-Neto Department of Pharmaceutical Sciences (DCF), Center of Health Sciences (CCS), Federal University of Paraíba (UFPB). Castelo Branco III, João Pessoa-PB.
  • Abrahão Oliveira-Filho Rural Tecnology and Heath Center (CSTR), Federal University of Campina Grande (UFCG). Jatobá, Patos - PB.
  • Edeltrudes Lima Department of Pharmaceutical Sciences (DCF), Center of Health Sciences (CCS), Federal University of Paraíba (UFPB). Castelo Branco III, João Pessoa-PB.
Introduction: Cladophialophora carrionii is one of the most frequent etiologic agents of human chromoblastomycosis, a chronic cutaneous disease. Such fungal infections are difficult to treat and underlines the need for new antifungal agents. Citral is a monoterpene with known pharmacological properties, including antimi-crobial activity. Aims: To investigate the antifungal activity of citral against strains of C. carrionii. Methodology: The minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were determined by broth microdilution techniques. Citral was tested to evaluate its effects on C. carrionii mycelia growth and germination of fungal conidia. Next, it was investigated the possible citral action on cell walls (sorbitol assay) and cell membranes (ergosterol binding assay). Results: The MIC50 and MFC50 of citral were respectively 128 μg/mL and 512 μg/mL. The study shows that citral displayed in vitro antifungal potential against strains of C. carrionii, where it was capable of inhibiting both its mycelial growth and germination of conidia for this fungus, whilst affecting the structure of fungal cell membranes. Citral’s mechanism of action involves binding to ergosterol. Further study is needed to completely describe its effects before clinical use as a therapeutic antifungal agent.

Introducción: Cladophialophora carrionii es uno de los agentes etiológicos más frecuentes de la cromoblastomicosis humana, una enfermedad cutánea crónica. Tales infecciones fúngicas son difíciles de tratar y subrayan la necesidad de nuevos agentes antifúngicos. Citral es un monoterpeno con propiedades farmacológicas conocidas, incluida la actividad antimicrobiana. Objetivo: investigar la actividad antifúngica de citral contra cepas de C. carrionii. Metodología: la concentración inhibitoria mínima (CIM) y la concentración fungicida mínima (CFM) se determinaron mediante técnicas de microdilución de caldo. Citral se probó para evaluar sus efectos sobre C. carrionii crecimiento de micelios y germinación de conidios fúngicos. A continuación, se investigó la posible acción citral sobre las paredes celulares (ensayo de sorbitol) y las membranas celulares (ensayo de unión al ergosterol). Resultados: La CIM50 y CFM50 de citral fueron respectivamente 128 μg/mL y 512 μg/mL. El estudio muestra que citral muestra potencial antifúngico in vitro contra cepas de C. carrionii, donde fue capaz de inhibir tanto su crecimiento micelial como la germinación de conidios para este hongo, a la vez que afectaba a la estructura de las membranas celulares fúngicas. El mecanismo de acción de Citral implica la unión al ergosterol. Se necesitan estudios adicionales para describir completamente sus efectos antes del uso clínico como agente antifúngico terapéutico.

Introducão: Cladophialophora carrionii é um dos agentes etiológicos mais frequentes da cromoblastomia humana, uma doença cutânea crônica. Tais infecções fúngicas são difíceis de tratar e sublinham a necessidade de novos agentes antifúngicos. Citral é um monoterpeno com propriedades farmacológicas conhecidas, incluindo atividade antimicrobiana. Objetivos: investigar a atividade antifúngica do citral contra cepas de C. carrionii. Metodologia: a concentração inibitória mínima (CIM) e a concentração fungicida mínima (CFM) foram determinadas por técnicas de microdiluição. Citral foi testado para avaliar seus efeitos em crescimento micelar e germinação conidialde C. carrionii. Em seguida, foi investigada a possível ação do citral na parede celular (ensaio de sorbitol) e membrana celular (ensaio de ligação ergosterol) fúngicas. Resultados: a CIM50 e CFM50 do citral foram respectivamente 128 μg/mL e 512 μg/mL. O estudo mostra que o citral exibiu potencial antifúngico in vitrocontra C. carrionii, onde era capaz de inibir tanto seu crescimento micelar quanto a germinação conidial, ao mesmo tempo em que afetava a estrutura da membrana celular fúngica. O mecanismo de ação do citral envolve a ligação ao ergosterol. Mais estudos são necessários para descrever completamente seus efeitos antes do uso clínico como um agente antifúngico terapêutico.

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Cómo citar

APA

de Menezes, C., Perez, A., Sousa, J., Ramos, K., Diniz-Neto, H., Oliveira-Filho, A. y Lima, E. (2023). Evaluation of the antifungal activity and mode of action of citral against Cladophialophora carrionii. Revista Colombiana de Ciencias Químico-Farmacéuticas, 51(3). https://doi.org/10.15446/rcciquifa.v51n3.100452

ACM

[1]
de Menezes, C., Perez, A., Sousa, J., Ramos, K., Diniz-Neto, H., Oliveira-Filho, A. y Lima, E. 2023. Evaluation of the antifungal activity and mode of action of citral against Cladophialophora carrionii. Revista Colombiana de Ciencias Químico-Farmacéuticas. 51, 3 (mar. 2023). DOI:https://doi.org/10.15446/rcciquifa.v51n3.100452.

ACS

(1)
de Menezes, C.; Perez, A.; Sousa, J.; Ramos, K.; Diniz-Neto, H.; Oliveira-Filho, A.; Lima, E. Evaluation of the antifungal activity and mode of action of citral against Cladophialophora carrionii. Rev. Colomb. Cienc. Quím. Farm. 2023, 51.

ABNT

DE MENEZES, C.; PEREZ, A.; SOUSA, J.; RAMOS, K.; DINIZ-NETO, H.; OLIVEIRA-FILHO, A.; LIMA, E. Evaluation of the antifungal activity and mode of action of citral against Cladophialophora carrionii. Revista Colombiana de Ciencias Químico-Farmacéuticas, [S. l.], v. 51, n. 3, 2023. DOI: 10.15446/rcciquifa.v51n3.100452. Disponível em: https://revistas.unal.edu.co/index.php/rccquifa/article/view/100452. Acesso em: 26 abr. 2025.

Chicago

de Menezes, Camilla, Ana Perez, Janiere Sousa, Katharina Ramos, Hermes Diniz-Neto, Abrahão Oliveira-Filho, y Edeltrudes Lima. 2023. «Evaluation of the antifungal activity and mode of action of citral against Cladophialophora carrionii». Revista Colombiana De Ciencias Químico-Farmacéuticas 51 (3). https://doi.org/10.15446/rcciquifa.v51n3.100452.

Harvard

de Menezes, C., Perez, A., Sousa, J., Ramos, K., Diniz-Neto, H., Oliveira-Filho, A. y Lima, E. (2023) «Evaluation of the antifungal activity and mode of action of citral against Cladophialophora carrionii»., Revista Colombiana de Ciencias Químico-Farmacéuticas, 51(3). doi: 10.15446/rcciquifa.v51n3.100452.

IEEE

[1]
C. de Menezes, «Evaluation of the antifungal activity and mode of action of citral against Cladophialophora carrionii»., Rev. Colomb. Cienc. Quím. Farm., vol. 51, n.º 3, mar. 2023.

MLA

de Menezes, C., A. Perez, J. Sousa, K. Ramos, H. Diniz-Neto, A. Oliveira-Filho, y E. Lima. «Evaluation of the antifungal activity and mode of action of citral against Cladophialophora carrionii». Revista Colombiana de Ciencias Químico-Farmacéuticas, vol. 51, n.º 3, marzo de 2023, doi:10.15446/rcciquifa.v51n3.100452.

Turabian

de Menezes, Camilla, Ana Perez, Janiere Sousa, Katharina Ramos, Hermes Diniz-Neto, Abrahão Oliveira-Filho, y Edeltrudes Lima. «Evaluation of the antifungal activity and mode of action of citral against Cladophialophora carrionii». Revista Colombiana de Ciencias Químico-Farmacéuticas 51, no. 3 (marzo 23, 2023). Accedido abril 26, 2025. https://revistas.unal.edu.co/index.php/rccquifa/article/view/100452.

Vancouver

1.
de Menezes C, Perez A, Sousa J, Ramos K, Diniz-Neto H, Oliveira-Filho A, Lima E. Evaluation of the antifungal activity and mode of action of citral against Cladophialophora carrionii. Rev. Colomb. Cienc. Quím. Farm. [Internet]. 23 de marzo de 2023 [citado 26 de abril de 2025];51(3). Disponible en: https://revistas.unal.edu.co/index.php/rccquifa/article/view/100452

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