Publicado

2024-01-22

Biofilm formation on toothbrushes by mutans group streptococci and Candida spp. isolated from oral cavity of students of the State University of Goiás, Brazil

Formación de biopelículas en cepillos de dientes por estreptococos del grupo mutans y Candida spp. aislados de cavidad oral de estudiantes de la Universidad Estatal de Goiás, Brasil

Formação de biofilme em escovas dentais por estreptococos do grupo mutans e Candida spp. isolados da cavidade oral de alunos da Universidade Estadual de Goiás, Brasil

Palabras clave:

Microbial biofilm, mouth, tooth decay, oral candidiasis, students (en)
Biopelícula microbiana, boca, caries dental, candidiasis oral, estudiantes (es)
Biofilme microbiano, boca, cárie dentária, candidíase bucal, estudantes (pt)

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Autores/as

  • Reuber Mendes Rocha Central Campus of the State University of Goiás
  • Plínio Lázaro Faleiro Naves Central Campus of the State University of Goiás

Introduction: Tooth brushing is an important measure in promoting oral health. However, the brush suffers the deposition and proliferation of microorganisms in its bristles during its use. In addition, a correlation has been demonstrated between the microbial load found on toothbrushes and the dental status of patients. Aim: To evaluate the biofilm formation on toothbrushes by mutans group streptococci and Candida spp. isolated from the oral cavity of students at the State University of Goiás. Methodology: The methodology employed in this study allowed the collection, isolation, and presumptive morphological identification of oral microorganisms, and allowed the evaluation of biofilm formation by both microorganisms in sterile toothbrushes. Results: All microbial isolates were able to form biofilms under the tested conditions, with microbial cell counts adhered to the biofilm ranging from 6.75 to 11.00 log10 cfu·mL-1 for mutans group streptococci, and 4.94 to 5.79 log10 cfu·mL-1 for Candida spp. Mutans group streptococci and Candida spp. were isolated from 92.31% of brushes used in the collection of samples from the oral cavity of the volunteers. Conclusion: All microorganisms isolated were able to form biofilm on toothbrushes, which corroborates the importance of care with decontamination of toothbrushes in promoting oral health.

Introducción: el cepillado de dientes es una medida importante en la promoción de la salud bucal. Sin embargo, el cepillo sufre la deposición y proliferación de microorganismos en sus cerdas durante su uso. Además, se demostró una correlación entre la carga microbiana encontrada en los cepillos de dientes y el estado dental de los pacientes. Objetivo: evaluar la formación de biofilm en cepillos de dientes por estreptococos del grupo mutans y Candida spp. aislados de la cavidad oral de estudiantes de la Universidad Estadual de Goiás. Metodología: la metodología utilizada en este estudio permitió la recolección, el aislamiento y la identificación morfológica presuntiva de los microorganismos orales, así como la evaluación de la formación de biofilms por los microorganismos en cepillos dentales estériles. Resultados: todos los aislados microbianos pudieron formar biofilms en las condiciones probadas, con recuentos de células microbianas adheridas a la biopelícula entre 6,75 11,00 log10 ufc·mL-1 para los estreptococos del grupo mutans y entre 4,94 - 5,79 log10 ufc·mL-1 para las Candida spp. Los estreptococos del grupo mutans y Candida spp. fueron aislados en el 92,31% de los cepillos utilizados para la toma de muestras de la cavidad bucal de los voluntarios. Conclusión: todos los microorganismos aislados fueron capaces de formar biofilms en los cepillos de dientes, lo que refuerza la importancia del cuidado de la descontaminación de los cepillos de dientes en la promoción de la salud bucal.

Introdução: a escovação dentária é uma medida importante na promoção da saúde bucal. No entanto, a escova sofre a deposição e proliferação de micro-organismos em suas cerdas durante o seu uso. Além disso, foi demonstrada uma correlação entre a carga microbiana encontrada nas escovas de dente e o estado dentário dos pacientes. Objetivo: avaliar a formação de biofilme em escovas dentais por estreptococos do grupo mutans e Candida spp. isolados da cavidade oral de estudantes da Universidade Estadual de Goiás. Metodologia: a metodologia empregada neste estudo permitiu a coleta, o isolamento e a identificação morfológica presuntiva de micro-organismos bucais, bem como a avaliação da formação de biofilme por ambos os micro-organismos em escovas dentais estéreis. Resultados: que todos os isolados microbianos foram capazes de formar biofilmes nas condições testadas, com contagens de células microbianas aderidas ao biofilme variando de 6,75 a 11,00 log10 ufc·mL-1 para estreptococos do grupo mutans e 4,94 a 5,79 log10 ufc·mL-1 para Candida spp. Foram isolados estreptococos do grupo mutans e Candida spp. em 92.31% das escovas usadas na coleta de amostras da cavidade bucal dos voluntários. Conclusão: todos os micro-organismos isolados foram capazes de formar biofilme nas escovas, o que reforça a importância dos cuidados com a descontaminação das escovas dentais na promoção da saúde bucal.

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Cómo citar

APA

Mendes Rocha, R. y Faleiro Naves, P. L. (2024). Biofilm formation on toothbrushes by mutans group streptococci and Candida spp. isolated from oral cavity of students of the State University of Goiás, Brazil. Revista Colombiana de Ciencias Químico-Farmacéuticas, 52(2). https://revistas.unal.edu.co/index.php/rccquifa/article/view/104480

ACM

[1]
Mendes Rocha, R. y Faleiro Naves, P.L. 2024. Biofilm formation on toothbrushes by mutans group streptococci and Candida spp. isolated from oral cavity of students of the State University of Goiás, Brazil. Revista Colombiana de Ciencias Químico-Farmacéuticas. 52, 2 (ene. 2024).

ACS

(1)
Mendes Rocha, R.; Faleiro Naves, P. L. Biofilm formation on toothbrushes by mutans group streptococci and Candida spp. isolated from oral cavity of students of the State University of Goiás, Brazil. Rev. Colomb. Cienc. Quím. Farm. 2024, 52.

ABNT

MENDES ROCHA, R.; FALEIRO NAVES, P. L. Biofilm formation on toothbrushes by mutans group streptococci and Candida spp. isolated from oral cavity of students of the State University of Goiás, Brazil. Revista Colombiana de Ciencias Químico-Farmacéuticas, [S. l.], v. 52, n. 2, 2024. Disponível em: https://revistas.unal.edu.co/index.php/rccquifa/article/view/104480. Acesso em: 9 ago. 2024.

Chicago

Mendes Rocha, Reuber, y Plínio Lázaro Faleiro Naves. 2024. «Biofilm formation on toothbrushes by mutans group streptococci and Candida spp. isolated from oral cavity of students of the State University of Goiás, Brazil». Revista Colombiana De Ciencias Químico-Farmacéuticas 52 (2). https://revistas.unal.edu.co/index.php/rccquifa/article/view/104480.

Harvard

Mendes Rocha, R. y Faleiro Naves, P. L. (2024) «Biofilm formation on toothbrushes by mutans group streptococci and Candida spp. isolated from oral cavity of students of the State University of Goiás, Brazil», Revista Colombiana de Ciencias Químico-Farmacéuticas, 52(2). Disponible en: https://revistas.unal.edu.co/index.php/rccquifa/article/view/104480 (Accedido: 9 agosto 2024).

IEEE

[1]
R. Mendes Rocha y P. L. Faleiro Naves, «Biofilm formation on toothbrushes by mutans group streptococci and Candida spp. isolated from oral cavity of students of the State University of Goiás, Brazil», Rev. Colomb. Cienc. Quím. Farm., vol. 52, n.º 2, ene. 2024.

MLA

Mendes Rocha, R., y P. L. Faleiro Naves. «Biofilm formation on toothbrushes by mutans group streptococci and Candida spp. isolated from oral cavity of students of the State University of Goiás, Brazil». Revista Colombiana de Ciencias Químico-Farmacéuticas, vol. 52, n.º 2, enero de 2024, https://revistas.unal.edu.co/index.php/rccquifa/article/view/104480.

Turabian

Mendes Rocha, Reuber, y Plínio Lázaro Faleiro Naves. «Biofilm formation on toothbrushes by mutans group streptococci and Candida spp. isolated from oral cavity of students of the State University of Goiás, Brazil». Revista Colombiana de Ciencias Químico-Farmacéuticas 52, no. 2 (enero 22, 2024). Accedido agosto 9, 2024. https://revistas.unal.edu.co/index.php/rccquifa/article/view/104480.

Vancouver

1.
Mendes Rocha R, Faleiro Naves PL. Biofilm formation on toothbrushes by mutans group streptococci and Candida spp. isolated from oral cavity of students of the State University of Goiás, Brazil. Rev. Colomb. Cienc. Quím. Farm. [Internet]. 22 de enero de 2024 [citado 9 de agosto de 2024];52(2). Disponible en: https://revistas.unal.edu.co/index.php/rccquifa/article/view/104480

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