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Preparation and characterization of copper nanoparticles of Schisandra chinensis and evaluation of its antiproliferative activity
preparación y caracterización de nanopartículas de cobre de Schisandra chinensis y evaluación de su actividad antiproliferativa
Preparação e caracterização de nanopartículas de cobre de Schisandra chinensis e avaliação de sua atividade antiproliferativa
DOI:
https://doi.org/10.15446/rcciquifa.v53n1.112982Palabras clave:
Schisandra chinensis, material science, nanotechnology, green synthesis, copper nanoparticles, antiproliferative effects, carcinoma (en)Schisandra chinensis, ciencia material, nanotecnología, síntesis verde, nanopartículas de cobre, efectos antiproliferativos, carcinoma (es)
Schisandra chinensis, ciência dos materiais, nanotecnologia, síntese verde, nanopartículas de cobre, efeitos antiproliferativos, carcinoma (pt)
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Introduction: Schisandra chinensis is a plant species whose fruits have been well known for its multiple pharmacological effects. In the current work, aqueous extract of S. chinensis fruits is used to produce Copper nanoparticles (CuNPs) through green-synthesis method. Physical and biological essays characterized the nanoparticles obtained. Aim: To produce Copper nanoparticles through a greensynthesis method using the aqueous extract of S. chinensis fruits and to characterize this material using spectroscopic methods, including UV-Vis, FTIR (Fourier Transform Infrared Spectra), X-ray diffraction, and SEM (Scanning electron microscopy). Subsequently, the nanoparticulate material is evaluated against three tumor cell lines: A549 human lung cancer cell line (CRM-CCL-185TM), HT29 human colorectal adenocarcinoma cell line (ATCC® HTB-38TM) and MCF7 breast cancer cell line (ATCC® HTB-22TM). Results: Through a green synthesis method, Copper nanoparticles were synthesized from aqueous extract of S. chinensis fruits, demonstrating a spherical morphology with a size close to 26 nanometers by means of spectral methods. Furthermore, results suggest that the S. chinensis reduced CuNPs were able to induce mainly early apoptotic cell death in cancer cells in a concentration-dependent manner. Conclusions: The results proved that S. chinensis fruit aqueous extract could be applied for a greener synthesis of copper nanoparticles with potential anti-proliferative effect.
Introducción: Schisandra chinensis es una especie vegetal cuyos frutos han sido muy conocidos por sus múltiples efectos farmacológicos. En el trabajo actual, se utiliza extracto acuoso de frutos de S. chinensis para producir nanopartículas de cobre (CuNP) mediante el método de síntesis verde. Ensayos físicos y biológicos caracterizaron las nanopartículas obtenidas. Objetivo: producir nanopartículas de cobre mediante un método de síntesis verde utilizando el extracto acuoso de frutos de S. chinensis y caracterizar este material mediante métodos espectroscópicos, incluidos UV-Vis, FTIR (espectros infrarrojos por transformada de Fourier), difracción de rayos X y SEM. (Microscopía electrónica de barrido). Posteriormente, el material nanoparticulado se evalúa frente a tres líneas celulares tumorales: línea celular de cáncer de pulmón humano A549 (CRM-CCL-185TM), línea celular de adenocarcinoma colorrectal humano HT29 (ATCC® HTB-38TM) y línea celular de cáncer de mama MCF7 (ATCC® HTB -22TM). Resultados: Mediante un método de síntesis verde, se sintetizaron nanopartículas de cobre a partir de extracto acuoso de frutos de S. chinensis, demostrando mediante métodos espectrales una morfología esférica con un tamaño cercano a los 26 nanómetros. Además, los resultados sugieren que las CuNP reducidas de S. chinensis fueron capaces de inducir principalmente la muerte celular apoptótica temprana en células cancerosas de una manera dependiente de la concentración. Conclusiones: Los resultados demostraron que el extracto acuoso del fruto de S. chinensis podría aplicarse para una síntesis más ecológica de nanopartículas de cobre con potencial efecto antiproliferativo.
Introdução: Schisandra chinensis é uma espécie vegetal cujos frutos são bastante conhecidos por seus múltiplos efeitos farmacológicos. No presente trabalho, extrato aquoso de frutos de S. chinensis é utilizado para produzir nanopartículas de cobre (CuNPs) através do método de síntese verde. Ensaios físicos e biológicos caracterizaram as nanopartículas obtidas. Objetivo: Produzir nanopartículas de cobre através de um método de síntese verde usando o extrato aquoso de frutos de S. chinensis e caracterizar este material usando métodos espectroscópicos, incluindo UV-Vis, FTIR (Fourier Transform Infrared Spectra), difração de raios X e MEV. (Microscopia eletrônica de varredura). Posteriormente, o material nanoparticulado é avaliado contra três linhagens de células tumorais: linhagem celular de câncer de pulmão humano A549 (CRM-CCL-185TM), linhagem celular de adenocarcinoma colorretal humano HT29 (ATCC® HTB-38TM) e linhagem celular de câncer de mama MCF7 (ATCC® HTB -22TM). Resultados: Através de um método de síntese verde, nanopartículas de cobre foram sintetizadas a partir de extrato aquoso de frutos de S. chinensis, demonstrando uma morfologia esférica com tamanho próximo a 26 nanômetros por meio de métodos espectrais. Além disso, os resultados sugerem que os CuNPs reduzidos de S. chinensis foram capazes de induzir principalmente a morte celular apoptótica precoce em células cancerosas de uma maneira dependente da concentração. Conclusões: Os resultados provaram que o extrato aquoso da fruta S. chinensis pode ser aplicado para uma síntese mais verde de nanopartículas de cobre com potencial efeito antiproliferativo.
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