Publicado

2024-06-06

Inhibitory effect of the essential oil of Schinus molle L. against pathogens causing periodontal disease

Efecto inhibitorio del aceite esencial de Schinus molle L. contra patógenos causantes de la enfermedad periodontal

Efeito inibitório do óleo essencial de Schinus molle L. contra patógenos causadores de doença periodontal

DOI:

https://doi.org/10.15446/rcciquifa.v53n2.114449

Palabras clave:

Oils, periodontal diseases, anti-bacterial agents (en)
Aceites, enfermedades periodontales, agentes antibacterianos (es)
Óleos, doenças periodontais, agentes antibacterianos (pt)

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Autores/as

  • Carlos Cadena-Viteri Specialty in Orthodontics, Catholic University of Cuenca, Azogues 030102, Ecuador;
  • Miriam Lima-Illescas Academic Unit of Health and Wellness, Faculty of Dentistry, Catholic University of Cuenca, Cuenca 010105, Ecuador;
  • Edisson-Mauricio Pacheco-Quito Academic Unit of Health and Wellness, Faculty of Dentistry, Catholic University of Cuenca, Cuenca 010105, Ecuador
  • Mariela Cumandá Balseca-Ibarra Central University of Ecuador. Quito 170521, Ecuador
  • Fernanda Sacoto-Figueroa Academic Unit of Health and Wellness, Faculty of Dentistry, Catholic University of Cuenca, Cuenca 010105, Ecuador;
  • Katherine Cuenca-León Pharmaceutical Sciences Department, University of Salamanca, 37007 Salamanca, Spain

Introduction: There are several oral diseases caused by various microorganisms. In this work, we discuss periodontal pathogens, which cause chronic degenerative damage in the supporting tissues of teeth. This is why several treatments have been developed for their eradication, including phytocomponents and essential oils as an option in antimicrobial therapy. Objective: The aim of this study was to determine the inhibitory effect of the essential oil of the plant species Schinus molle L. native to Ecuador on strain of Porphyromonas gingivalis at different concentrations. Methodology: This was a laboratory and longitudinal study in which the Porphyromonas gingivalis ATCC 33277 strain was cultured in 20 Petri dishes, working with several exposure subgroups, including Group 1 - 50% essential oil of Schinus molle leaves; Group 2 - 100% essential oil of Schinus molle L.; Group 3 - 0.12% chlorhexidine (positive control); Group 4 - saline solution (negative control) with different incubation periods of 24 and 72 hours. Results: The Porphyromonas gingivalis ATCC 33277 sample exposed to 100% plant species Schinus molle L. for 24 hours had an inhibition zone of 15 mm, demonstrating high sensitivity, and exposure for 72 hours produced a zone of 14 mm, also suggesting sensitivity. Exposure to S. molle L. at 50% for 24 hours produced a zone of inhibition of 9.65 mm, showing sensitivity; however, it is worthwhile to continue developing and evaluating this area of study. Conclusions: This study demonstrates that phytotherapy using the essential oil of the plant species Schinus molle L. represents a therapeutic option in cases of infections caused by Porphyromonas gingivalis.

Introducción: Existen varias enfermedades orales causadas por diversos microorganismos. En este trabajo, discutimos los patógenos periodontales, que causan daños crónicos degenerativos en los tejidos de soporte de los dientes. Por ello, se han desarrollado diversos tratamientos para su erradicación, entre los que destacan los fitocomponentes y aceites esenciales como opción en la terapia antimicrobiana. Objetivo: El objetivo de este estudio fue determinar el efecto inhibitorio del aceite esencial de la especie vegetal Schinus molle L. nativa del Ecuador sobre una cepa de Porphyromonas gingivalis a diferentes concentraciones. Metodología: Se trató de un estudio de laboratorio y longitudinal en el que se cultivó la cepa Porphyromonas gingivalis ATCC 33277 en 20 placas Petri, se trabajó con varios subgrupos de exposición, tales como: Grupo 1 - 50% aceite esencial de hojas de Schinus molle; Grupo 2 - 100% aceite esencial de Schinus molle L.; Grupo 3 - clorhexidina al 0,12% (control positivo); Grupo 4 - solución salina (control negativo) a diferentes periodos de incubación de 24 a 72 horas. Resultados: La muestra de Porphyromonas gingivalis ATCC 33277 expuesta al 100% de la especie vegetal Schinus molle L. durante 24 horas presentó un halo de inhibición de 15 mm, lo que demuestra una alta sensibilidad, y la exposición durante 72 horas produjo un halo de 14 mm, lo que también sugiere sensibilidad. La exposición a S. molle L. al 50% durante 24 horas produjo un halo de inhibición de 9,65 mm, demostrando sensibilidad; sin embargo, vale la pena continuar desarrollando y evaluando esta área de estudio. Conclusiones: Este estudio demuestra que la fitoterapia utilizando el aceite esencial de la especie vegetal Schinus molle L. representa una opción terapéutica, en casos de infecciones ocasionadas por Porphyromonas gingivalis.

Introdução: Existem diversas doenças bucais causadas por diversos microrganismos. Neste trabalho, discutimos os patógenos periodontais, que causam danos degenerativos crônicos aos tecidos de suporte dos dentes. Por este motivo, vários tratamentos têm sido desenvolvidos para a sua erradicação, entre os quais se destacam os fitocomponentes e os óleos essenciais como opção na terapia antimicrobiana. Objetivo: O objetivo deste estudo foi determinar o efeito inibitório do óleo essencial da espécie vegetal Schinus molle L. nativa do Equador sobre uma cepa de Porphyromonas gingivalis em diferentes concentrações. Metodologia: Trata-se de um estudo laboratorial e longitudinal em que a cepa Porphyromonas gingivalis ATCC 33277 foi cultivada em 20 placas de Petri, trabalhando com diversos subgrupos de exposição, tais como: Grupo 1 - 50% de óleo essencial de folhas de Schinus molle L.; Grupo 2 - óleo essencial 100% de Schinus molle L.; Grupo 3 - clorexidina 0,12% (controle positivo); Grupo 4 - solução salina (controle negativo) em diferentes períodos de incubação de 24 a 72 horas. Resultados: A amostra de Porphyromonas gingivalis ATCC 33277 exposta a 100% da espécie vegetal Schinus molle L. por 24 horas apresentou zona de inibição de 15 mm, o que demonstra alta sensibilidade, e a exposição por 72 horas produziu zona de inibição de 14 mm, o que também sugere sensibilidade. A exposição a S. molle L. a 50% durante 24 horas produziu uma zona de inibição de 9,65 mm, demonstrando sensibilidade; no entanto, vale a pena continuar a desenvolver e avaliar esta área de estudo. Conclusões: Este estudo demonstra que a fitoterapia utilizando o óleo essencial da espécie vegetal Schinus molle L. representa uma opção terapêutica em casos de infecções causadas por Porphyromonas gingivalis.

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Cómo citar

APA

Cadena-Viteri, C., Lima-Illescas, M., Pacheco-Quito, E.-M., Balseca-Ibarra, M. C., Sacoto-Figueroa, F. y Cuenca-León, K. (2024). Inhibitory effect of the essential oil of Schinus molle L. against pathogens causing periodontal disease. Revista Colombiana de Ciencias Químico-Farmacéuticas, 53(2), 414–429. https://doi.org/10.15446/rcciquifa.v53n2.114449

ACM

[1]
Cadena-Viteri, C., Lima-Illescas, M., Pacheco-Quito, E.-M., Balseca-Ibarra, M.C., Sacoto-Figueroa, F. y Cuenca-León, K. 2024. Inhibitory effect of the essential oil of Schinus molle L. against pathogens causing periodontal disease. Revista Colombiana de Ciencias Químico-Farmacéuticas. 53, 2 (jun. 2024), 414–429. DOI:https://doi.org/10.15446/rcciquifa.v53n2.114449.

ACS

(1)
Cadena-Viteri, C.; Lima-Illescas, M.; Pacheco-Quito, E.-M.; Balseca-Ibarra, M. C.; Sacoto-Figueroa, F.; Cuenca-León, K. Inhibitory effect of the essential oil of Schinus molle L. against pathogens causing periodontal disease. Rev. Colomb. Cienc. Quím. Farm. 2024, 53, 414-429.

ABNT

CADENA-VITERI, C.; LIMA-ILLESCAS, M.; PACHECO-QUITO, E.-M.; BALSECA-IBARRA, M. C.; SACOTO-FIGUEROA, F.; CUENCA-LEÓN, K. Inhibitory effect of the essential oil of Schinus molle L. against pathogens causing periodontal disease. Revista Colombiana de Ciencias Químico-Farmacéuticas, [S. l.], v. 53, n. 2, p. 414–429, 2024. DOI: 10.15446/rcciquifa.v53n2.114449. Disponível em: https://revistas.unal.edu.co/index.php/rccquifa/article/view/114449. Acesso em: 16 ago. 2024.

Chicago

Cadena-Viteri, Carlos, Miriam Lima-Illescas, Edisson-Mauricio Pacheco-Quito, Mariela Cumandá Balseca-Ibarra, Fernanda Sacoto-Figueroa, y Katherine Cuenca-León. 2024. «Inhibitory effect of the essential oil of Schinus molle L. against pathogens causing periodontal disease». Revista Colombiana De Ciencias Químico-Farmacéuticas 53 (2):414-29. https://doi.org/10.15446/rcciquifa.v53n2.114449.

Harvard

Cadena-Viteri, C., Lima-Illescas, M., Pacheco-Quito, E.-M., Balseca-Ibarra, M. C., Sacoto-Figueroa, F. y Cuenca-León, K. (2024) «Inhibitory effect of the essential oil of Schinus molle L. against pathogens causing periodontal disease», Revista Colombiana de Ciencias Químico-Farmacéuticas, 53(2), pp. 414–429. doi: 10.15446/rcciquifa.v53n2.114449.

IEEE

[1]
C. Cadena-Viteri, M. Lima-Illescas, E.-M. Pacheco-Quito, M. C. Balseca-Ibarra, F. Sacoto-Figueroa, y K. Cuenca-León, «Inhibitory effect of the essential oil of Schinus molle L. against pathogens causing periodontal disease», Rev. Colomb. Cienc. Quím. Farm., vol. 53, n.º 2, pp. 414–429, jun. 2024.

MLA

Cadena-Viteri, C., M. Lima-Illescas, E.-M. Pacheco-Quito, M. C. Balseca-Ibarra, F. Sacoto-Figueroa, y K. Cuenca-León. «Inhibitory effect of the essential oil of Schinus molle L. against pathogens causing periodontal disease». Revista Colombiana de Ciencias Químico-Farmacéuticas, vol. 53, n.º 2, junio de 2024, pp. 414-29, doi:10.15446/rcciquifa.v53n2.114449.

Turabian

Cadena-Viteri, Carlos, Miriam Lima-Illescas, Edisson-Mauricio Pacheco-Quito, Mariela Cumandá Balseca-Ibarra, Fernanda Sacoto-Figueroa, y Katherine Cuenca-León. «Inhibitory effect of the essential oil of Schinus molle L. against pathogens causing periodontal disease». Revista Colombiana de Ciencias Químico-Farmacéuticas 53, no. 2 (junio 6, 2024): 414–429. Accedido agosto 16, 2024. https://revistas.unal.edu.co/index.php/rccquifa/article/view/114449.

Vancouver

1.
Cadena-Viteri C, Lima-Illescas M, Pacheco-Quito E-M, Balseca-Ibarra MC, Sacoto-Figueroa F, Cuenca-León K. Inhibitory effect of the essential oil of Schinus molle L. against pathogens causing periodontal disease. Rev. Colomb. Cienc. Quím. Farm. [Internet]. 6 de junio de 2024 [citado 16 de agosto de 2024];53(2):414-29. Disponible en: https://revistas.unal.edu.co/index.php/rccquifa/article/view/114449

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