Publicado

2019-01-01

CdCl2 has zero-order kinetic cellular influx and induces cytotoxicity and genotoxicity at low concentrations in human leukocytes in vitro

El CdCl2 tiene un influjo celular cinético de orden cero e induce citotoxicidad y genotoxicidad a bajas concentraciones en leucocitos humanos in vitro

Palabras clave:

Cadmium, cellular damage, DNA damage, defense cells (en)
Cadmio, daño celular, daño en el ADN, células de defensa (es)

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Autores/as

  • Vanessa Fontoura dos Santos Universidade Federal do Pampa (UNIPAMPA)
  • Elvio Adílio Serpa Universidade Federal do Pampa (UNIPAMPA)
  • Fabiana Ernestina Barcellos da Silva Universidade Federal do Pampa (UNIPAMPA)
  • Érico Marlon de Moraes Flores Universidade Federal de Santa Maria (UFSM) - Chemistry Department
  • Michel Mansur Machado Universidade Federal do Pampa (UNIPAMPA)
  • Luís Flávio Souza de Oliveira Universidade Federal do Pampa (UNIPAMPA)
Cadmium (Cd2+) is a nonessential heavy metal that possesses a high capacity of bioaccumulation and exhibits toxic characteristics even at low concentrations. This study evaluated the genotoxicity and cytotoxicity in human leukocytes in vitro after exposure to a lower range of Cd2+concentration (1-25 μg/mL) using an unprecedented strategy by correlating between intracellular Cd2+ levels after exposure and cellular damage. Results demonstrated that Cd2+exposure from 5 to 25 μg/mL significantly increased the unviability of leukocytes, as well as the DNA damage, which was dose-dependent. The intracellular Cd2+ levels in leukocytes ranged from 9.85 to 94.38 pg/cell, and cytotoxicity and genotoxicity were induced at a concentration of 24.22 pg/cell. The relationship between exposure concentration and intracellular Cd2+ levels suggests that its influx occurs in human leukocytes under zeroorder kinetics.
El cadmio (Cd2+) es un metal pesado no esencial que posee una alta capacidad de bioacumulación y presenta características tóxicas incluso en bajas concentraciones. Este estudio evaluó la genotoxicidad y la citotoxicidad en leucocitos humanos in vitro después de la exposición a un rango inferior de concentración de Cd2+ (1-25 μg / mL) mediante una estrategia sin precedentes al correlacionar los niveles intracelulares de Cd2+ después de la exposición y el daño celular. Los resultados demostraron que la exposición a Cd2+ de 5 a 25 μg/mL aumentó significativamente la inviabilidad de los leucocitos, así como el daño en el ADN, que era dependiente de la dosis. Los niveles intracelulares de Cd2+ en leucocitos oscilaron entre 9,85 y 94,38 pg/célula, y se indujo la citotoxicidad y la genotoxicidad a una concentración de 24,22 pg/ célula. La relación entre la concentración de la exposición y los niveles intracelulares de Cd2+ sugiere que su influjo se produce en leucocitos humanos bajo una cinética de orden cero.

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