Publicado

2020-05-01

Study of the antifungal activity of ibuprofen and its association with amphotericin B or ketoconazole against Candida spp.

Estudio de la actividad antifúngica del ibuprofeno y su asociación con anfotericina B o ketoconazol contra Candida spp.

Estudo da atividade antifúngica do ibuprofeno e sua associação com anfotericina B ou cetoconazol contra Candida spp.

DOI:

https://doi.org/10.15446/rcciquifa.v49n2.89516

Palabras clave:

Candida, ibuprofen, antifungals, association (en)
Candida, ibuprofeno, antifúngicos, associação (pt)
Candida, ibuprofeno, antifúngicos, asociación (es)

Autores/as

  • Paula Mariane Silva Sousa Academic Unit of Health, Education and Health Center, Federal University of Campina Grande, 58175-000, Cuité, Paraíba
  • Jefferson Rodrigues Nóbrega Academic Unit of Health, Education and Health Center, Federal University of Campina Grande, 58175-000, Cuité, Paraíba
  • Laísa Vilar Cordeiro PhD student of Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos at Universidade Federal da Paraíba, 58033-455, João Pessoa, Paraíba
  • Francisco Patricio de Andrade Júnio Master in natural and synthetic bioactive products at Universidade Federal da Paraíba, 58033-455, João Pessoa, Paraíba
  • Wylly Araújo de Oliveira Professor PhD at Universidade Federal de Campina Grande, 58429-900, Cuité, Paraíba

The objective was evaluating the antifungal activity of ibuprofen alone and when associated with amphotericin B or ketoconazole against Candida species. Strains of C. albicans, C. tropicalis, C. guilliermondii, C. krusei and C. parapsilosis were used. The minimum inhibitory concentration (MIC) was determined by the microdilution method and the association study performed through the checkerboard assay. The concentration of 512 µg/mL inhibited approximately 65% of the tested strains, while against 35% of the strains presented MIC values above 2048 µg/mL. Associations of ibuprofen with amphotericin B against C. tropicalis and ibuprofen with ketoconazole against C. krusei showed synergistic effect. Antagonistic effects were evidenced in the combination of ibuprofen with amphotericin B against C. guilliermondii and C. albicans, as well as in the association of ibuprofen with ketoconazole against C. albicans and C. tropicalis. Through the experiments, it was found that ibuprofen showed antifungal activity against most of the Candida species tested. The combinations of ibuprofen and antifungals had synergistic effects. However, antagonistic results were evidenced in the association with ibuprofen, which would make clinical applicability difficult. Therefore, studies of this combined activity should be investigated, considering that this association may be positive for antifungal therapy.

El objetivo fue evaluar la actividad antifúngica del ibuprofeno solo y asociado con anfotericina B o ketoconazol contra especies de Candida. Se utilizaron cepas de C. albicans, C. tropicalis, C. guilliermondii, C. krusei y C. parapsilosis. La concentración inhibitoria mínima (MIC) se determinó mediante el método de microdilución y el estudio de asociación fue realizado a través del ensayo de checkboard. La concentración de 512 µg/mL inhibió aproximadamente el 65% de las cepas analizadas, mientras 35% de las cepas presentaron valores de MIC superiores a 2048 µg/mL. Las asociaciones de ibuprofeno con anfotericina B contra C. tropicalis e ibuprofeno con ketoconazol contra C. krusei mostraron un efecto sinérgico. Se evidenciaron efectos antagonistas en la combinación de ibuprofeno con anfotericina B contra C. guilliermondii y C. albicans, así como en la asociación de ibuprofeno con ketoconazol contra C. albicans y C. tropicalis. Se descubrió, a través de los experimentos, que el ibuprofeno mostró actividad antifúngica contra la mayoría de las especies de Candida probadas. Las combinaciones de ibuprofeno y antifúngicos tuvieron efectos sinérgicos. Sin embargo, se evidenciaron resultados antagónicos en la asociación con ibuprofeno, lo que dificultaría la aplicabilidad clínica. Por lo tanto, los estudios de esta actividad combinada deben investigarse, considerando que esta asociación puede ser positiva para la terapia antimicótica.

O objetivo do estudo foi avaliar a atividade antifúngica do ibuprofeno sozinho e quando associado com anfotericina B ou cetoconazol contra espécies de Candida. Foram utilizadas cepas fúngicas de C. albicans, C. tropicalis, C. guilliermondii, C. krusei e C. parapsilosis, entre isolados clínicos e cepas padrão. A concentração inibitória mínima (CIM) foi determinada pela técnica de microdiluição e o estudo de associação realizado através do ensaio checkerboard. A concentração de 512 µg/mL foi capaz de inibir, aproximadamente, 65% das cepas ensaiadas, enquanto que 35% das estirpes apresentaram valores da CIM acima de 2048 µg/mL. Associações do ibuprofeno com anfotericina B contra C. tropicalis e ibuprofeno mais cetoconazol contra C. krusei mostraram efeito sinérgico. Efeitos antagônicos foram evidenciados na combinação do ibuprofeno com anfotericina B contra C. guilliermondii e C. albicans, como também na associação do ibuprofeno com cetoconazol contra C. albicans e C. tropicalis. Por meio dos experimentos, pôde-se afirmar que o ibuprofeno exerceu atividade antifúngica contra a maioria das espécies de Candida ensaiadas. Os efeitos das combinações entre o ibuprofeno e os antifúngicos promoveram efeitos sinérgicos. No entanto, resultados antagônicos foram evidenciados na associação com o ibuprofeno, o que dificultaria aplicabilidade clínica. Logo, estudos dessa atividade combinada devem ser investigados, pois esta associação pode traçar pontos positivos na terapia antifúngica.

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Cómo citar

APA

Silva Sousa, P. M., Rodrigues Nóbrega, J., Vilar Cordeiro, L., de Andrade Júnio, F. P. y Araújo de Oliveira, W. (2020). Study of the antifungal activity of ibuprofen and its association with amphotericin B or ketoconazole against Candida spp. Revista Colombiana de Ciencias Químico-Farmacéuticas, 49(2). https://doi.org/10.15446/rcciquifa.v49n2.89516

ACM

[1]
Silva Sousa, P.M., Rodrigues Nóbrega, J., Vilar Cordeiro, L., de Andrade Júnio, F.P. y Araújo de Oliveira, W. 2020. Study of the antifungal activity of ibuprofen and its association with amphotericin B or ketoconazole against Candida spp. Revista Colombiana de Ciencias Químico-Farmacéuticas. 49, 2 (may 2020). DOI:https://doi.org/10.15446/rcciquifa.v49n2.89516.

ACS

(1)
Silva Sousa, P. M.; Rodrigues Nóbrega, J.; Vilar Cordeiro, L.; de Andrade Júnio, F. P.; Araújo de Oliveira, W. Study of the antifungal activity of ibuprofen and its association with amphotericin B or ketoconazole against Candida spp. Rev. Colomb. Cienc. Quím. Farm. 2020, 49.

ABNT

SILVA SOUSA, P. M.; RODRIGUES NÓBREGA, J.; VILAR CORDEIRO, L.; DE ANDRADE JÚNIO, F. P.; ARAÚJO DE OLIVEIRA, W. Study of the antifungal activity of ibuprofen and its association with amphotericin B or ketoconazole against Candida spp. Revista Colombiana de Ciencias Químico-Farmacéuticas, [S. l.], v. 49, n. 2, 2020. DOI: 10.15446/rcciquifa.v49n2.89516. Disponível em: https://revistas.unal.edu.co/index.php/rccquifa/article/view/89516. Acesso em: 7 oct. 2024.

Chicago

Silva Sousa, Paula Mariane, Jefferson Rodrigues Nóbrega, Laísa Vilar Cordeiro, Francisco Patricio de Andrade Júnio, y Wylly Araújo de Oliveira. 2020. «Study of the antifungal activity of ibuprofen and its association with amphotericin B or ketoconazole against Candida spp». Revista Colombiana De Ciencias Químico-Farmacéuticas 49 (2). https://doi.org/10.15446/rcciquifa.v49n2.89516.

Harvard

Silva Sousa, P. M., Rodrigues Nóbrega, J., Vilar Cordeiro, L., de Andrade Júnio, F. P. y Araújo de Oliveira, W. (2020) «Study of the antifungal activity of ibuprofen and its association with amphotericin B or ketoconazole against Candida spp»., Revista Colombiana de Ciencias Químico-Farmacéuticas, 49(2). doi: 10.15446/rcciquifa.v49n2.89516.

IEEE

[1]
P. M. Silva Sousa, J. Rodrigues Nóbrega, L. Vilar Cordeiro, F. P. de Andrade Júnio, y W. Araújo de Oliveira, «Study of the antifungal activity of ibuprofen and its association with amphotericin B or ketoconazole against Candida spp»., Rev. Colomb. Cienc. Quím. Farm., vol. 49, n.º 2, may 2020.

MLA

Silva Sousa, P. M., J. Rodrigues Nóbrega, L. Vilar Cordeiro, F. P. de Andrade Júnio, y W. Araújo de Oliveira. «Study of the antifungal activity of ibuprofen and its association with amphotericin B or ketoconazole against Candida spp». Revista Colombiana de Ciencias Químico-Farmacéuticas, vol. 49, n.º 2, mayo de 2020, doi:10.15446/rcciquifa.v49n2.89516.

Turabian

Silva Sousa, Paula Mariane, Jefferson Rodrigues Nóbrega, Laísa Vilar Cordeiro, Francisco Patricio de Andrade Júnio, y Wylly Araújo de Oliveira. «Study of the antifungal activity of ibuprofen and its association with amphotericin B or ketoconazole against Candida spp». Revista Colombiana de Ciencias Químico-Farmacéuticas 49, no. 2 (mayo 1, 2020). Accedido octubre 7, 2024. https://revistas.unal.edu.co/index.php/rccquifa/article/view/89516.

Vancouver

1.
Silva Sousa PM, Rodrigues Nóbrega J, Vilar Cordeiro L, de Andrade Júnio FP, Araújo de Oliveira W. Study of the antifungal activity of ibuprofen and its association with amphotericin B or ketoconazole against Candida spp. Rev. Colomb. Cienc. Quím. Farm. [Internet]. 1 de mayo de 2020 [citado 7 de octubre de 2024];49(2). Disponible en: https://revistas.unal.edu.co/index.php/rccquifa/article/view/89516

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1. Ksenia V. Drozd, Denis E. Boycov, Alex N. Manin, Andrei V. Churakov, German L. Perlovich. (2023). Two Novel Drug–Drug Cocrystals of Antifungal Clotrimazole with Nonsteroidal Anti-Inflammatory Drugs. Crystal Growth & Design, 23(9), p.6597. https://doi.org/10.1021/acs.cgd.3c00520.

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