Publicado

2020-05-01

Talinum paniculatum: a plant with antifungal potential mitigates fluconazole-induced oxidative damage-mediated growth inhibition of Candida albicans

Talinum paniculatum: una planta con potencial antifúngico atenúa la inhibición del crecimiento de Candida albicans mediada por el daño oxidativo inducido por fluconazol

DOI:

https://doi.org/10.15446/rcciquifa.v49n2.89704

Palabras clave:

Antimicrobial resistance, Candida albicans, Susceptibility, Reactive oxygen species, Talinum paniculatum, fluconazole (en)
Resistencia a los antimicrobianos, Candida albicans, especies reactivas de oxígeno, Talinum paniculatum, fluconazole (es)

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Autores/as

  • Cláudio Daniel Cerdeira Department of Biochemistry (DBc), Institute of Biomedical Sciences (IBS), Federal University of Alfenas (UNIFAL-MG), Alfenas, 37130-001, Minas Gerais (MG)
  • Jeferson J. da Silva Laboratory of Microbiology and Immunology, Department of Oral Diagnostic, Dental school of Piracicaba, State University of Campinas (FOP/UNICAMP), Piracicaba, 13414-903, São Paulo (SP)
  • Manoel F. R. Netto Laboratory of Microbiology and Immunology, Department of Oral Diagnostic, Dental school of Piracicaba, State University of Campinas (FOP/UNICAMP), Piracicaba, 13414-903, São Paulo (SP)
  • Marcelo F. G. Boriollo Laboratory of Microbiology and Immunology, Department of Oral Diagnostic, Dental school of Piracicaba, State University of Campinas (FOP/UNICAMP), Piracicaba, 13414-903, São Paulo
  • Gabriel O. I. Moraes Department of Biochemistry (DBc), Institute of Biomedical Sciences (IBS), Federal University ofAlfenas (UNIFAL-MG), Alfenas, 37130-001, Minas Gerais (MG)
  • Gérsika B. Santos Department of Biochemistry (DBc), Institute of Biomedical Sciences (IBS), Federal University of Alfenas (UNIFAL-MG), Alfenas, 37130-001, Minas Gerais (MG)
  • Luis F. C. dos Reis Laboratory of Medicinal Plants, UNIFAL-MG, Alfenas, 37130-001, MG
  • Maísa R. P. L. Brigagão Department of Biochemistry (DBc), Institute of Biomedical Sciences (IBS), Federal University of Alfenas (UNIFAL-MG), Alfenas, 37130-001, Minas Gerais (MG

Aims: This study investigated the bioactivity of the crude leaf extract (CLE) and fractions hexane (HX) and ethyl acetate (EtOAc) from Talinum paniculatum alone and in association with fluconazole (FLC) against reference strain and clinical isolates of FLC-resistant Candida albicans. Furthermore, the antioxidant capability, chemical composition of this plant, and the effect’s underlying mechanisms were evaluated. Methods: The antifungal activity was evaluated using checkerboard assay to establish the minimum inhibitory (MIC) and minimum microbicidal concentrations (MMC). During FLC and plant products challenges, the reactive oxygen species (ROS) generation (hydroxyl radicals [HO•]) were detected in C. albicans cells using the membrane-permeable fluorescent probes APF and HPF. High-performance liquid chromatography (HPLC) profile, quantitative analysis of antioxidant compounds, and free radical scavenging activity (DPPH assay) tests were performed. Results: The CLE and fractions presented outstanding antifungal activity and selectivity against C. albicans cells but had no synergistic effects with FLC. The MIC values for CLE and its fractions against C. albicans reference strain were in the order of HX (31.25 μg ml–1) < EtOAc (62.5 μg ml–1) < CLE (500 μg ml–1), and against FLC-resistant C. albicans HX (125 μg ml–1) = EtOAc < CLE (500 μg ml–1). CLE and its fractions had more potent antifungal activities than FLC against the clinical isolates. Moreover, fungicidal effects for these plant products were demonstrated against FLC-resistant C. albicans, which further confirmed an antifungal potential. Conversely, during association, plant products were shown to cause an increase in FLC MIC anywhere from 2- to 16-fold. FLC exposure led to an increase in the steady-state levels of ROS (HO•) in C. albicans cells. Next, we found that the increases in FLC MICs were owing to action of antioxidants containing-CLE and its fractions in preventing FLC-induced ROS-mediated growth inhibition of C. albicans. Conclusion: T. paniculatum can be a source of bioactive compounds with antifungal potential. However, because of the common use of its edible leaf, caution is advised during therapy with FLC (since it can decrease FLC susceptibility).

 

Objetivos: este estudio investigó la bioactividad del extracto de hoja en bruto (EHB) y las fracciones hexano (HX) y acetato de etilo (AcOEt) de Talinum paniculatum solo y en asociación con fluconazol (FLC) contra cepas de referencia y aislados clínicos de Candida albicans resistente a FLC. Además, evaluó la capacidad antioxidante, la composición química de esta planta y los mecanismos subyacentes del efecto fungicida. Métodos: la actividad antifúngica se evaluó mediante microdilución en caldo para establecer las concentraciones inhibitorias mínimas (CIM) y microbicidas mínimas (CMM). Durante el tratamiento con FLC y productos vegetales se detectó la generación de especies reactivas de oxígeno (ERO) (radicales hidroxilo [HO•]) en células de C. albicans utilizando las sondas fluorescentes permeables a la membrana APF y HPF. El perfil de cromatografía líquida de alta resolución (CLAR), el análisis cuantitativo de compuestos antioxidantes y el ensayo DPPH fueron evaluados. Resultados: el EHB y las fracciones presentaron una excelente actividad antifúngica y selectividad contra las células de C. albicans, pero no tuvieron efectos sinérgicos con FLC. Los valores de CIM para EHB y sus fracciones contra la cepa referencia de C. albicans fueron del orden de: HX (31,25 μg ml–1) < AcOEt (62,5 μg ml–1) < EHB (500 μg ml–1), y contra C. albicans resistente a FLC: HX (125 μg ml–1)= AcOEt < EHB (500 μg ml–1). EHB y sus fracciones fueron más potentes antifúngicos que FLC contra los aislados clínicos. Además, estos productos vegetales tienen efectos fungicidas contra C. albicans resistentes a FLC, esto confirmó el potencial antifúngico. Por el contrario, durante la asociación se demostró que los productos vegetales causan un aumento en la CIM de FLC de 2 a 16 veces. La exposición a FLC aumentó los niveles de ERO (HO•) en las células de C. albicans. Los aumentos en las CIM de FLC se debieron a la acción de los antioxidantes presentes en EHB y sus fracciones para prevenir la inhibición del crecimiento mediada por ERO inducida por FLC en C. albicans. Conclusión: T. paniculatum puede ser una fuente de compuestos bioactivos con potencial antifúngico. Sin embargo, debido al uso común de su hoja comestible, se recomienda usarla con precaución durante la terapia con FLC (ya que puede disminuir la susceptibilidad a FLC).

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Cómo citar

APA

Cerdeira, C. D., da Silva, J. J., R. Netto, M. F., G. Boriollo, M. F., I. Moraes, G. O., Santos, G. B., C. dos Reis, L. F. y P. L. Brigagão, M. R. (2020). Talinum paniculatum: a plant with antifungal potential mitigates fluconazole-induced oxidative damage-mediated growth inhibition of Candida albicans. Revista Colombiana de Ciencias Químico-Farmacéuticas, 49(2). https://doi.org/10.15446/rcciquifa.v49n2.89704

ACM

[1]
Cerdeira, C.D., da Silva, J.J., R. Netto, M.F., G. Boriollo, M.F., I. Moraes, G.O., Santos, G.B., C. dos Reis, L.F. y P. L. Brigagão, M.R. 2020. Talinum paniculatum: a plant with antifungal potential mitigates fluconazole-induced oxidative damage-mediated growth inhibition of Candida albicans. Revista Colombiana de Ciencias Químico-Farmacéuticas. 49, 2 (may 2020). DOI:https://doi.org/10.15446/rcciquifa.v49n2.89704.

ACS

(1)
Cerdeira, C. D.; da Silva, J. J.; R. Netto, M. F.; G. Boriollo, M. F.; I. Moraes, G. O.; Santos, G. B.; C. dos Reis, L. F.; P. L. Brigagão, M. R. Talinum paniculatum: a plant with antifungal potential mitigates fluconazole-induced oxidative damage-mediated growth inhibition of Candida albicans. Rev. Colomb. Cienc. Quím. Farm. 2020, 49.

ABNT

CERDEIRA, C. D.; DA SILVA, J. J.; R. NETTO, M. F.; G. BORIOLLO, M. F.; I. MORAES, G. O.; SANTOS, G. B.; C. DOS REIS, L. F.; P. L. BRIGAGÃO, M. R. Talinum paniculatum: a plant with antifungal potential mitigates fluconazole-induced oxidative damage-mediated growth inhibition of Candida albicans. Revista Colombiana de Ciencias Químico-Farmacéuticas, [S. l.], v. 49, n. 2, 2020. DOI: 10.15446/rcciquifa.v49n2.89704. Disponível em: https://revistas.unal.edu.co/index.php/rccquifa/article/view/89704. Acesso em: 16 jul. 2024.

Chicago

Cerdeira, Cláudio Daniel, Jeferson J. da Silva, Manoel F. R. Netto, Marcelo F. G. Boriollo, Gabriel O. I. Moraes, Gérsika B. Santos, Luis F. C. dos Reis, y Maísa R. P. L. Brigagão. 2020. «Talinum paniculatum: a plant with antifungal potential mitigates fluconazole-induced oxidative damage-mediated growth inhibition of Candida albicans». Revista Colombiana De Ciencias Químico-Farmacéuticas 49 (2). https://doi.org/10.15446/rcciquifa.v49n2.89704.

Harvard

Cerdeira, C. D., da Silva, J. J., R. Netto, M. F., G. Boriollo, M. F., I. Moraes, G. O., Santos, G. B., C. dos Reis, L. F. y P. L. Brigagão, M. R. (2020) «Talinum paniculatum: a plant with antifungal potential mitigates fluconazole-induced oxidative damage-mediated growth inhibition of Candida albicans», Revista Colombiana de Ciencias Químico-Farmacéuticas, 49(2). doi: 10.15446/rcciquifa.v49n2.89704.

IEEE

[1]
C. D. Cerdeira, «Talinum paniculatum: a plant with antifungal potential mitigates fluconazole-induced oxidative damage-mediated growth inhibition of Candida albicans», Rev. Colomb. Cienc. Quím. Farm., vol. 49, n.º 2, may 2020.

MLA

Cerdeira, C. D., J. J. da Silva, M. F. R. Netto, M. F. G. Boriollo, G. O. I. Moraes, G. B. Santos, L. F. C. dos Reis, y M. R. P. L. Brigagão. «Talinum paniculatum: a plant with antifungal potential mitigates fluconazole-induced oxidative damage-mediated growth inhibition of Candida albicans». Revista Colombiana de Ciencias Químico-Farmacéuticas, vol. 49, n.º 2, mayo de 2020, doi:10.15446/rcciquifa.v49n2.89704.

Turabian

Cerdeira, Cláudio Daniel, Jeferson J. da Silva, Manoel F. R. Netto, Marcelo F. G. Boriollo, Gabriel O. I. Moraes, Gérsika B. Santos, Luis F. C. dos Reis, y Maísa R. P. L. Brigagão. «Talinum paniculatum: a plant with antifungal potential mitigates fluconazole-induced oxidative damage-mediated growth inhibition of Candida albicans». Revista Colombiana de Ciencias Químico-Farmacéuticas 49, no. 2 (mayo 1, 2020). Accedido julio 16, 2024. https://revistas.unal.edu.co/index.php/rccquifa/article/view/89704.

Vancouver

1.
Cerdeira CD, da Silva JJ, R. Netto MF, G. Boriollo MF, I. Moraes GO, Santos GB, C. dos Reis LF, P. L. Brigagão MR. Talinum paniculatum: a plant with antifungal potential mitigates fluconazole-induced oxidative damage-mediated growth inhibition of Candida albicans. Rev. Colomb. Cienc. Quím. Farm. [Internet]. 1 de mayo de 2020 [citado 16 de julio de 2024];49(2). Disponible en: https://revistas.unal.edu.co/index.php/rccquifa/article/view/89704

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CrossRef citations2

1. Cláudio Daniel Cerdeira, Maísa R. P. L. Brigagão. (2024). Targeting Macrophage Polarization in Infectious Diseases: M1/M2 Functional Profiles, Immune Signaling and Microbial Virulence Factors. Immunological Investigations, , p.1. https://doi.org/10.1080/08820139.2024.2367682.

2. Bruno Fonsêca Feitosa, Charlene Maria de Alcântara, Amanda Beatriz Sales de Lima, Adriano Sant’Ana Silva, Alfredina dos Santos Araújo, Mônica Tejo Cavalcanti, Edna Mori, Isaac Moura Araújo, Pablo Antonio Maia de Farias, Polrat Wilairatana, Henrique Douglas Melo Coutinho. (2022). Bioactive Natural Products for Chemical Control of Microorganisms: Scientific Prospecting (2001–2021) and Systematic Review. Molecules, 27(18), p.5917. https://doi.org/10.3390/molecules27185917.

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