Publicado

2020-05-01

Talinum paniculatum leaves with in vitro antimicrobial activity against reference and clinical strains of Staphylococcus aureus interfere in oxacillin action

Las hojas de Talinum paniculatum con actividad antimicrobiana in vitro contra cepas de referencia y clínicas de Staphylococcus aureus interfieren con la acción de la oxacilina

DOI:

https://doi.org/10.15446/rcciquifa.v49n2.89894

Palabras clave:

Antimicrobial resistance, Staphylococcus aureus, reactive oxygen species, oxacillin, Talinum paniculatum (en)
Resistencia a los antimicrobianos, Staphylococcus aureus, oxacilina, Talinum paniculatum (es)

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Autores/as

  • Cláudio Daniel Cerdeira Department of Biochemistry (DBc), Institute of Biomedical Sciences (IBS), Federal University of Alfenas (UNIFAL-MG), Alfenas, 37130-001, Minas Gerais (MG)
  • Jeferson J. da Silva Laboratory of Microbiology and Immunology, Department of Oral Diagnostic, Dental school of Piracicaba, State University of Campinas (FOP/UNICAMP), Piracicaba, 13414-903, São Paulo (SP)
  • Manoel F. R. Netto Laboratory of Microbiology and Immunology, Department of Oral Diagnostic, Dental school of Piracicaba, State University of Campinas (FOP/UNICAMP), Piracicaba, 13414-903, São Paulo
  • Marcelo F. G. Boriollo Laboratory of Microbiology and Immunology, Department of Oral Diagnostic, Dental school of Piracicaba, State University of Campinas (FOP/UNICAMP), Piracicaba, 13414-903, São Paulo
  • Gérsika B. Santos Department of Biochemistry (DBc), Institute of Biomedical Sciences (IBS), Federal University of Alfenas (UNIFAL-MG), Alfenas, 37130-001, Minas Gerais (MG)
  • Luis F. C. dos Reis Laboratory of Medicinal Plants, UNIFAL-MG, Alfenas, 37130-001, MG
  • Maísa R. P. L. Brigagão Department of Biochemistry (DBc), Institute of Biomedical Sciences (IBS), Federal University of Alfenas (UNIFAL-MG), Alfenas, 37130-001, Minas Gerais (MG)

Propose: We evaluated the antibacterial potential of the crude leaf extract (CLE) and fractions hexane (HX) and ethyl acetate (EtOAc) from Talinum paniculatum alone and in association with oxacillin (OXA) against OXA-resistant Staphylococcus aureus (ORSA, environment isolates) and OXA-sensitive S. aureus (OSSA, ATCC 25923). Furthermore, toxicity tests were performed. Methods: The antibacterial activity was evaluated through checkerboard assay (broth microdilution) to establish the minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC). Toxicity test in mice was assessed. Results: The MIC values for the CLE and its fractions against ORSA and OSSA were in the order of HX (500 μg ml–1) = EtOAc < CLE (4000 μg ml–1). EtOAc and HX presented outstanding antibacterial activities against ORSA, and these fractions were bactericidal toward OSSA. Conversely, the associations between plant product (CLE, EtOAc, or HX) and OXA exhibited no synergistic effects. During these associations, there was an increase in OXA MICs anywhere from 2- to 4092-fold. The CLE presented absence of toxicity at a dose of 5 g kg-1 (in vivo). Conclusion: Although T. paniculatum be a good source of bioactive compounds with antistaphylococcal potential, the researchers should be cautious, since its edible leaf may interfere with OXA therapy (mitigating OXA-induced growth inhibition or killing of S. aureus and enhancing S. aureus resistance).

Propósito: evaluamos el potencial antibacteriano del extracto de hoja en bruto (EHB) y las fracciones hexano (HX) y acetato de etilo (AcOEt) de Talinum paniculatum solo y en asociación con oxacilina (OXA) contra Staphylococcus aureus resistente a OXA (ORSA, ambientales) y S. aureus sensible a OXA (OSSA, ATCC 25923). Además, se realizaron pruebas de toxicidad. Métodos: la actividad antibacteriana se evaluó mediante microdilución en caldo para establecer las concentraciones inhibitorias mínimas (CIM) y bactericidas mínimas (CBM). Se evaluó la toxicidad en ratones. Resultados: los valores de CIM para el EHB y sus fracciones contra ORSA y OSSA fueron del orden de HX (500 μg ml–1) = AcOEt < EHB (4000 μg ml–1). AcOEt y HX presentaron actividades antibacterianas sobresalientes contra ORSA, y estas fracciones fueron bactericidas hacia OSSA. Por el contrario, las asociaciones entre el producto vegetal (EHB, AcOEt o HX) y OXA no mostraron efectos sinérgicos. Durante estas asociaciones, hubo un aumento en las CIM de OXA de 2 a 4092 veces. EHB no mostró toxicidad a una dosis de 5 g kg–1. Conclusión: aunque T. paniculatum es una buena fuente de compuestos bioactivos con potencial antiestofilocócico, los investigadores deben ser cautelosos, ya que su hoja comestible puede interferir con la terapia con OXA (mitigando la inhibición del crecimiento inducida por OXA o la muerte de S. aureus y promoviendo resistencia bacteriana).

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Cómo citar

APA

Cerdeira, C. D., da Silva, J. J., R. Netto, M. F., G. Boriollo, M. F., Santos, G. B., C. dos Reis, L. F. y P. L. Brigagão, M. R. (2020). Talinum paniculatum leaves with in vitro antimicrobial activity against reference and clinical strains of Staphylococcus aureus interfere in oxacillin action. Revista Colombiana de Ciencias Químico-Farmacéuticas, 49(2). https://doi.org/10.15446/rcciquifa.v49n2.89894

ACM

[1]
Cerdeira, C.D., da Silva, J.J., R. Netto, M.F., G. Boriollo, M.F., Santos, G.B., C. dos Reis, L.F. y P. L. Brigagão, M.R. 2020. Talinum paniculatum leaves with in vitro antimicrobial activity against reference and clinical strains of Staphylococcus aureus interfere in oxacillin action. Revista Colombiana de Ciencias Químico-Farmacéuticas. 49, 2 (may 2020). DOI:https://doi.org/10.15446/rcciquifa.v49n2.89894.

ACS

(1)
Cerdeira, C. D.; da Silva, J. J.; R. Netto, M. F.; G. Boriollo, M. F.; Santos, G. B.; C. dos Reis, L. F.; P. L. Brigagão, M. R. Talinum paniculatum leaves with in vitro antimicrobial activity against reference and clinical strains of Staphylococcus aureus interfere in oxacillin action. Rev. Colomb. Cienc. Quím. Farm. 2020, 49.

ABNT

CERDEIRA, C. D.; DA SILVA, J. J.; R. NETTO, M. F.; G. BORIOLLO, M. F.; SANTOS, G. B.; C. DOS REIS, L. F.; P. L. BRIGAGÃO, M. R. Talinum paniculatum leaves with in vitro antimicrobial activity against reference and clinical strains of Staphylococcus aureus interfere in oxacillin action. Revista Colombiana de Ciencias Químico-Farmacéuticas, [S. l.], v. 49, n. 2, 2020. DOI: 10.15446/rcciquifa.v49n2.89894. Disponível em: https://revistas.unal.edu.co/index.php/rccquifa/article/view/89894. Acesso em: 14 ago. 2024.

Chicago

Cerdeira, Cláudio Daniel, Jeferson J. da Silva, Manoel F. R. Netto, Marcelo F. G. Boriollo, Gérsika B. Santos, Luis F. C. dos Reis, y Maísa R. P. L. Brigagão. 2020. «Talinum paniculatum leaves with in vitro antimicrobial activity against reference and clinical strains of Staphylococcus aureus interfere in oxacillin action». Revista Colombiana De Ciencias Químico-Farmacéuticas 49 (2). https://doi.org/10.15446/rcciquifa.v49n2.89894.

Harvard

Cerdeira, C. D., da Silva, J. J., R. Netto, M. F., G. Boriollo, M. F., Santos, G. B., C. dos Reis, L. F. y P. L. Brigagão, M. R. (2020) «Talinum paniculatum leaves with in vitro antimicrobial activity against reference and clinical strains of Staphylococcus aureus interfere in oxacillin action», Revista Colombiana de Ciencias Químico-Farmacéuticas, 49(2). doi: 10.15446/rcciquifa.v49n2.89894.

IEEE

[1]
C. D. Cerdeira, «Talinum paniculatum leaves with in vitro antimicrobial activity against reference and clinical strains of Staphylococcus aureus interfere in oxacillin action», Rev. Colomb. Cienc. Quím. Farm., vol. 49, n.º 2, may 2020.

MLA

Cerdeira, C. D., J. J. da Silva, M. F. R. Netto, M. F. G. Boriollo, G. B. Santos, L. F. C. dos Reis, y M. R. P. L. Brigagão. «Talinum paniculatum leaves with in vitro antimicrobial activity against reference and clinical strains of Staphylococcus aureus interfere in oxacillin action». Revista Colombiana de Ciencias Químico-Farmacéuticas, vol. 49, n.º 2, mayo de 2020, doi:10.15446/rcciquifa.v49n2.89894.

Turabian

Cerdeira, Cláudio Daniel, Jeferson J. da Silva, Manoel F. R. Netto, Marcelo F. G. Boriollo, Gérsika B. Santos, Luis F. C. dos Reis, y Maísa R. P. L. Brigagão. «Talinum paniculatum leaves with in vitro antimicrobial activity against reference and clinical strains of Staphylococcus aureus interfere in oxacillin action». Revista Colombiana de Ciencias Químico-Farmacéuticas 49, no. 2 (mayo 1, 2020). Accedido agosto 14, 2024. https://revistas.unal.edu.co/index.php/rccquifa/article/view/89894.

Vancouver

1.
Cerdeira CD, da Silva JJ, R. Netto MF, G. Boriollo MF, Santos GB, C. dos Reis LF, P. L. Brigagão MR. Talinum paniculatum leaves with in vitro antimicrobial activity against reference and clinical strains of Staphylococcus aureus interfere in oxacillin action. Rev. Colomb. Cienc. Quím. Farm. [Internet]. 1 de mayo de 2020 [citado 14 de agosto de 2024];49(2). Disponible en: https://revistas.unal.edu.co/index.php/rccquifa/article/view/89894

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1. Cláudio Daniel Cerdeira, Maísa R. P. L. Brigagão. (2024). Targeting Macrophage Polarization in Infectious Diseases: M1/M2 Functional Profiles, Immune Signaling and Microbial Virulence Factors. Immunological Investigations, , p.1. https://doi.org/10.1080/08820139.2024.2367682.

2. Marina Silalahi, Anisatu Z. Wakhidah. (2023). RETRACTED ARTICLE: The food plants trade in the Kranggan Mas traditional market, West Java Province, Indonesia: food security and local cuisine. Journal of Ethnic Foods, 10(1) https://doi.org/10.1186/s42779-023-00192-5.

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