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The selective obtaining of amyrins from Amazonian Protium oleoresins
La obtención selectiva de amirinas a partir de oleorresinas de Protium amazónicas
DOI:
https://doi.org/10.15446/rcciquifa.v49n2.89923Palabras clave:
Protium, alpha and beta-amyrins, yielding extraction, extraction optimization (en)Protium, amirinas alfa y beta, rendimientos de extracción, optimización de extracción (es)
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The oleoresin produced by species of genus Protium sp. is rich in alpha and beta-amyrins, two triterpenes with many pharmacogical activities. Considering the need to make the improved obtainment of these products feasible, this study sought to optimize techniques for the extraction and isolation of amyrins from resin. Two methods of extraction (maceration and sonication) with different solvents were compared to direct isolation from crude resin. The isolation of triterpenes was performed by chromatographic columns and the yields of extracts and fractions were analyzed by analysis of variance. The best extraction solvent for amyrins was hexane for both maceration and sonication methods (38.16±2.06% and 37.67±8.21%, respectively). There was no statistical difference between these methods and the direct method (32.05±2.40%). Additionally, the direct method is cheaper and more environmentally friendly. Thus, this study showed that it is possible to obtain a large quantity of amyrins by means of cheap, fast and ecological methods.
La oleorresina producida por especies del género Protium sp. es rica en amirinas alfa y beta, dos triterpenos con muchas actividades farmacológicas. Esta investigación buscó optimizar las técnicas de extracción y aislamiento de amirinas de la resina para hacer factible la obtención mejorada de esos productos. Se compararon dos métodos de extracción (maceración y sonicación) con diferentes solventes con aislamiento directo de la resina cruda. El aislamento de los triterpenos se realizó mediante columnas cromatográficas y los rendimientos de extractos y fracciones fueron hechos mediante análisis de varianza. El mejor solvente para la extracción de amirinas fue el hexano para ambos métodos de maceración y sonicación (38,16±2,06% y 37,67±8,21%, respectivamente). No hubo diferencia estadística entre estos métodos y el método directo (32,05±2,40%). Además, el método directo es más barato y ecológico. De este modo, esta investigación demostró que es posible obtener una gran cantidad de amirinas a través de métodos rápidos, baratos y ecológicos.
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1. Laise de Jesus dos Santos, Elesandra da Silva Araujo, Mário Sérgio Lorenço, Bianca Bueno Rosário, Sabrina Benmuyal Vieira, Agust Sales, Marco Antonio Siviero, Luiz Eduardo de Lima Melo, Graciene da Silva Mota, Gabriela Aguiar Campolina, Fabio Akira Mori. (2024). Characterization of the bark of Protium tenuifolium Engl. from the Amazonian biome as a source of natural antioxidants. Holzforschung, 78(1), p.16. https://doi.org/10.1515/hf-2023-0039.
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