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El modelo de ovario artificial como prueba celular para evaluar la seguridad de los extractos de Spirulina maxima y Kéfir: un estudio preliminar
he model of artificial ovary as a cellular test to evaluate the safety of Spirulina máxima and Kefir extracts: a preliminary study
DOI:
https://doi.org/10.15446/rcciquifa.v49n3.91342Palabras clave:
pruebas sin animales, efectos adversos, suplemento dietético, probiótico, bienestar, folículo preantral (es)Non animal testing, adverse effect, dietary supplement, pre-biotic, wellness, preantral follicle (en)
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S. maxima y Kefir son conocidos y utilizados por sus propiedades antioxidantes e inmunoestimulantes. El objetivo en este estudio fue evaluar los extractos naturales
de estos dos agentes con la técnica de manipulación de ovocitos incluidos en folículos preantrales (MFP), porque la técnica puede reemplazar el uso de animales de
laboratorio y también podría armonizar las leyes que intentan reducir y mejorar el uso de animales para el estudio de nuevos fármacos y para integrar a las buenas prácticas de laboratorio. Los extractos naturales estudiados fueron obtenidos a partir de S. maxima y Kéfir, las dos sustancias son conocidas en el mercado por su actividad
antioxidante e inmunoestimulante. Los dos extractos fueron evaluados en suspensiones de 10, 50, 100 and 200 µg.mL-1. Los resultados muestran que Spirulina
produjo disminución en la sobrevivencia, el desarrollo y el diámetro folicular. Mientras que Kéfir no mostró influencias positivas o negativas sobre el crecimiento y desarrollo de los folículos preantrales, sólo la concentración de 200 µg.mL-1 disminuyó la sobrevivencia folicular. La técnica MFP demostró encajar en la política de las 3R
(reemplazo, reducción y refinamiento) y permitió evaluar la citotoxicidad mostrando que la técnica puede ser usada como una prueba de seguridad en extractos naturales.
S. maxima and Kéfir are known and used for their antioxidant and immunostimulant properties. This study aimed to evaluate the natural extracts of these two
agents with the technique of manipulation of oocytes included in preantral follicles (MFP), because the technique can replace the use of laboratory animals and could
also harmonize the laws that try to reduce and improve the use of animals for the study of new drugs and to integrate good laboratory practices. The natural extracts
studied were obtained from S. maxima and Kefir, both substances are known in the market for their antioxidant and immunostimulating activity. Both were evaluated in suspensions of 10, 50, 100 and 200 µg.mL-1. The results show that Spirulina produced a decrease in survival, development, and follicular diameter. While Kefir
did not show positive or negative influences on the growth and development of preantral follicles, only the concentration of 200 µg.mL-1 decreased follicular
survival. The MFP technique proved to fit into the 3R policy (replacement, reduction, and refinement) and allowed to evaluate cytotoxicity, showing that the technique can be used as a safety test in natural extracts.
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