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Determination of phenolic bioactive compounds and evaluation of the antioxidant and hemolytic activities in the methanolic extracts of Tradescantia zebrina
Determinación de compuestos bioactivos fenólicos y evaluación de las actividades antioxidante y hemolítica en los extractos metanólicos de Tradescantia zebrina
Determinação de compostos fenólicos bioativos e avaliação das atividades antioxidante e hemolítica em extratos metanólicos de Tradescantia zebrina
DOI:
https://doi.org/10.15446/rcciquifa.v51n3.101403Keywords:
Tradescantia zebrina, antioxidants, phenolic compounds, HPLC-DAD, biological activity (en)Tradescantia zebrina, antioxidantes, compuestos fenólicos, HPLC-DAD, actividad biológica (es)
Tradescantia zebrina, antioxidantes, compostos fenólicos, HPLC-DAD, atividade biológica (pt)
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Introducción: Tradescantia zebrina es una especie utilizada principalmente en Suda-mérica, hay pocos reportes sobre su composición química y bioactividad. Objetivo: determinar compuestos bioactivos fenólicos, por primera vez en la literatura, y las actividades antioxidante y hemolítica de extractos (secos e in natura) de esta especie. Métodos: los extractos se obtuvieron por maceración en metanol y los contenidos de fenoles y flavonoides totales se expresaron en mg equivalentes de ácido gálico (EAG)/g y quercetina (EQ) de extracto, respectivamente. El potencial antioxidante fue evaluado por los métodos de captura de radicales DPPH• y ABTS•+. La identifica-ción y cuantificación de compuestos bioactivos fenólicos se realizó por cromatografía líquida de alta resolución acoplada a detector de diodo (HPLC-DAD) y el ensayo de actividad hemolítica se realizó por difusión en agar sangre, utilizando la técnica de antiobiograma de disco. Resultados: los contenidos de fenoles y flavonoides totales en los extractos (in natura y secos) fueron 67,68 y 233,94 mg EAG/g y 29,70 y 10,99 mg EQ/g de extracto concentrado, respectivamente. Los ácidos fenólicos (cafeico, elágico, ferúlico, ρ-cumárico y protocatequiico), los flavonoides (crisina y rutina) y un aldehído fenólico (vainillina) se determinaron mediante cromatografía líquida de alta resolución-detector de diodos (HPLC-DAD). Las actividades antioxidantes fueron confirmadas por la inhibición de los radicales DPPH• (44,08%) y ABTS•+(46,99%) para muestras in natura, respectivamente. Conclusión: los extractos metanólicos de Tradescantia zebrina probados no presentaron toxicidades cuando se probaron para la actividad hemolítica después de 72 horas. Por lo tanto, Tradescantia zebrina es una fuente potencial de sustancias bioactivas.
Introdução: Tradescantia zebrina é uma espécie utilizada principalmente na América do Sul, há poucos relatos sobre sua composição química e bioatividade. Objetivo: determinar compostos bioativos fenólicos, pela primeira vez na literatura, e as atividades antioxidante e hemolítica de extratos (secos e in natura) desta espécie.Métodos: os extratos foram obtidos por maceração em methanol e os teores de compostos fenólicos e flavonóides totais foram expressos em mg equivalente de ácido gálico (EAG)/g e quercetina (EQ) de extrato, respectivamente. O potencial antioxidante foi avaliado pelos métodos de captura do radical DPPH• e ABTS•+. A identificação e quantificação de compostos bioativos fenólicos foi realizada por cromatografia líquida de alta eficiência acoplada a detector de diodo, na região do ultravioleta e o ensaio de atividade hemolítica foi realizado por difusão em ágar sangue, pela técnica de antiobiograma em discos. Resultados: os teores de fenóis totais e flavonoides nos extratos (in natura e seco) foram 67,68 e 233,94 mg EAG/g e 29,70 e 10,99 mg EQ/g de extrato concentrado, respectivamente. Ácidos fenólicos (caféico, elágico, ferúlico, ρ-cumárico e protocatecúico), flavonoides (crisina e rutina) e um aldeído fenólico (vanilina) foram determinados por cromatografia líquida de alta eficiência-detector de diodo (CLAE-DAD). As atividades antioxidantes foram confirmadas pela inibição dos radicais DPPH• (44,08%) e ABTS•+ (46,99%) para amostras in natura, respectivamente. Conclusão: os extratos metanólicos de Tradescantia zebrina testados não apresentaram toxicidade quando testados para atividade hemolítica após 72 horas. Portanto, Tradescantia zebrina é uma fonte potencial de substâncias bioativas.
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