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Biodesulfurización de queroseno con células inmovilizadas de Pseudomonas sp. No. 6 (aislado nativo) en un novedoso biorreactor de lecho de tubos con recirculación
KEROSENE BIODESULFURIZATION BY IMMOBILIZED CELLS Pseudomonas sp. NO. 6 (NATIVE ISOLATED) AT A NEW BIOREACTOR OF PIPE BED WITH RECIRCULATION
Keywords:
biodesulfurizacion, inmovilización celular, Pseudomonas (es)biodesulfurization, immobilized cell, Pseudomonas. (en)
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En este trabajo se realizó la biodesulfurización de queroseno con células inmovilizadas de Pseudomonas sp. ATCC 39327, en un sistema emulsionado, W/O 30/70 (v/v), con medio mínimo de sales (MMS) como fase dispersa. El sistema de inmovilización consta de dos paneles de 60 tubos cada uno. Los tubos de alta alúmina tienen 140 mm de largo, 6,6 mm de diámetro externo y 3,2 mm de diámetro interno, con una porosidad del 43% y capacidad de retención de 9,5x109 células/g de soporte, colocados dentro de una columna de vidrio enchaquetada, de 80 por 400 mm de zona de reacción, conectada a un tanque de recirculación, y el fluido se mueve con aire a razón de 8,8 pies3/h. Después de 120 horas de proceso a 30 ºC y presión atmosférica se obtuvo una remoción del contenido de azufre de 43% y una pérdida calórica menor del 2%.
In this work, the kerosene biodesulfurization was developed by immobilized cells of Pseudomonas spp. ATCC 39327, in an emulsioned system W/O 30/70 (v/v) with sales minimal medium as disperse phase. The immobilization system has two panels of 60 pipes each one. The “Alta Alumina†pipes has 140 mm long, 6,6 mm of external diameter and internal diameter 3,2 mm, with 43% porosity and retention capacity of 9,5x109 cells/g of support, put into jacked column, with a reaction zone of 80 by 400 mm of reaction zone, connected to recirculation tank, and the fluid is moved by air at 8,8 feet 3 /h. The process rendered a sulfur removal of 43% with a caloric lost of less than 2,0%, after 120 hours of treatment at 30 ºC and normal pressure.
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