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Chemical composition and larvicidal activity of essential oil of the bark of Citrus sinensis (L.) Osbeck
Composición química y actividad larvicida del aceite esencial de corteza de Citrus sinensis (L.) Osbeck
Composição química e atividade larvicida do óleo essencial das cascas do Citrus sinensis (L.) Osbeck
DOI:
https://doi.org/10.15446/rcciquifa.v50n1.95448Keywords:
Aedes aegypti, larvae, volatile compounds, Citrus sinensis (L.) Osbeck, distilled oil (en)Aedes aegypti, larvas, compuestos volátiles, Citrus sinensis (L.) Osbeck, aceite destilado (es)
Aedes aegypti, compostos voláteis, Citrus sinensis (L.) Osbeck, óleo destilado, larvas (pt)
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Aim: We determined the chemical composition and larvicidal activity of the essential oil distilled from the Citrus sinensis (L.) Osbeck husks and the pattern of the major constituent. Materials and methods: For this, we distill the oil by hydrodistillation, identify the components by gas chromatography coupled with mass spectrometry (GC-MS), test the larvicidal activity against Aedes aegypti and calculate the lethal concentration at 50% (LC50) by the Reed-Muench method and the confidence interval by the Pizzi method for both oil and standard. Results: it showed that the oil consists mostly of limonene and showed larvicidal activity (LC50 of 199.01(± 2.10) μg·mL-1) greater than the lemonade standard (126.03 (± 2.09) μg·mL-1). Conclusion: Therefore, we conclude that distilled oil has the potential to replace chemical larvicides.
Objetivo: determinamos la composición química y actividad larvicida del aceite esencial destilado de las cáscaras de Citrus sinensis (L.) Osbeck y el patrón del constituyente mayoritario. Materiales y métodos: para ello destilamos el aceite por hidrodestilación, identificamos los componentes mediante cromatografía de gases acoplada a espectrometría de masas (CG-EM), testeamos la actividad larvicida frente a Aedes aegypti y calculamos la concentración letal al 50% (CL50) por el método Reed- Muench y el intervalo de confianza por el método de Pizzi tanto para aceite como para la muestra estándar. Resultados: el aceite consiste principalmente en limoneno y mostró actividad larvicida (CL50 de 199,01 (± 2,10) μg·mL-1) mayor que el estándar de limonada (126,03 (± 2,09) μg·mL-1). Conclusiones: por lo tanto, concluimos que el aceite destilado tiene el potencial de reemplazar los larvicidas químicos.
Objetivo: determinamos a composição química e a atividade larvicida do óleo essencial destilado das cascas do Citrus sinensis (L.) Osbeck e do padrão do constituinte majoritário. Materiais e métodos: para isso, destilamos o óleo por hidrodestilação, identificamos os componentes por cromatografia gasosa acoplada à espectrometria de massas (CG-EM), testamos a atividade larvicida contra o Aedes aegypti e calculamos a concentração letal a 50% (CL50) pelo método Reed-Muench e o intervalo de confiança pelo método de Pizzi tanto para o óleo quanto para o padrão. Resultados: o óleo é constituído em sua maior parte por limoneno e apresentou atividade larvicida (CL50 de 199.01 (± 2,10) μg·mL-1) maior que o padrão limonento (126,03 (± 2,09) μg·mL-1). Conclusões: portanto, concluímos que o óleo destilado tem potencial para substituir os larvicida químicos.
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