Publicado

2024-06-06

An in vivo and in silico predictive study on the toxicological and modulatory effects of abused substances on sperm quality and testicular function in Wistar rats

Un estudio predictivo in vivo e in silico sobre los efectos toxicológicos y moduladores de las sustancias de abuso sobre la calidad del esperma y la función testicular en ratas Wistar

Um estudo preditivo in vivo e in silico sobre os efeitos toxicológicos e moduladores de substâncias de abuso na qualidade do esperma e na função testicular em ratos Wistar

DOI:

https://doi.org/10.15446/rcciquifa.v53n2.114454

Palabras clave:

Wistar rats, Sperm Motility, Sodium Glutamate, Tramadol, Menthol, Follicle Stimulating Hormone (en)
ratas Wistar, motilidad de los espermatozoides, glutamato de sodio, tramadol, mentol, hormona folículo estimulante (es)
Ratos Wistar, motilidade espermática, glutamato de sódio, tramadol, mentol, hormônio folículo‑estimulante (pt)

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Autores/as

  • Charles Obiora Nwonuma Department of Biochemistry, College of Pure and Applied Sciences, Landmark University P.M.B. 1001, Omu Aran, Kwara State, Nigeria.
  • Adeola Oluwaseun Adedoyin Department of Biochemistry, College of Pure and Applied Sciences, Landmark University P.M.B. 1001, Omu Aran, Kwara State, Nigeria
  • Melody Onyemaka Department of Biochemistry, College of Pure and Applied Sciences, Landmark University P.M.B. 1001, Omu Aran, Kwara State, Nigeria
  • Emenike Irokanulo Department of Chemistry, University of Lagos, Lagos State, Nigeria
  • Omokolade Oluwaseyi Alejolowo Department of Biochemistry, College of Pure and Applied Sciences, Landmark University P.M.B. 1001, Omu Aran, Kwara State, Nigeria
  • Inemesit Asukwo Udofia Department of Food Science and Microbiology, College of Pure and Applied Sciences, Landmark University P.M.B. 1001, Omu Aran, Kwara State, Nigeria
  • Oluwafemi Adeleke Ojo Phytomedicine, Molecular Toxicology, and Computational Biochemistry Research Laboratory (PMTCB‑RL), Department of Biochemistry, Bowen University, Iwo, Nigeria
  • Deborah A. Adah Department of Veterinary Physiology and Biochemistry, University of Ilorin, Ilorin, Nigeria
  • Funmilayo Abimbola Okeniyi Department of Animal Science, Landmark University, Omu‑Aran, Nigeria
  • Omorefosa O. Osemwegie Department of Food Science and Microbiology, College of Pure and Applied Sciences, Landmark University P.M.B. 1001, Omu Aran, Kwara State, Nigeria.

Introduction: Some compounds like Opioids that are commonly used may affect the biological system in addition to having a high potential for addiction. Objective: This study assessed the effects of commonly misused substances on sperm quality and testicular function in Wistar rats. Material and Methods: Twenty-five Wistar rats weighing an average of 120 ± 0.1 g were randomly assigned to five treatment groups and were orally administered with water for the control, carbonated sugar drink, 150, 300, and 300 mg/kg body weight doses of menthol, monosodium glutamate, and tramadol respectively. The rats were euthanized 24 hours after the last day of the thirty-day treatment. Biochemical assays were on carried out the plasma and testicular homogenate. Results: There was a significant increase (p<0.05) in testosterone, FSH, LH, HDL, TG, phospholipids, glycogen, reduced glutathione concentration, sperm total count; %testicular weight change, and; there was also a significant decrease in the %tail defect, and %non-motile sperm across the treatment groups compared to the control. Contrary, there was a significant increase (p<0.05) in the testicular ACP and Na-K ATPases activities but MDA levels decreased significantly across treatment groups. The ouabain-α-ATPase complex’s binding energy is comparable to that of the α-ATPase complexes with tramadol, glucose, menthol, and MSG, respectively. Conclusion: The improved sperm quality and testicular function show that these compounds were not harmful to the reproductive functions of Wistar rats. The docking analysis corroborated the effects of ATPase activity modulation on sperm motility.

Introducción: Algunos compuestos como los opioides, que se usan comúnmente, pueden afectar el sistema biológico, además de tener un alto potencial de adicción. Objetivo: Este estudio evaluó los efectos de sustancias comúnmente utilizadas indebidamente sobre la calidad del esperma y la función testicular en ratas Wistar. Material y métodos: Veinticinco ratas Wistar con un peso promedio de 120 ± 0,1 g fueron asignadas aleatoriamente a cinco grupos de tratamiento y se les administró por vía oral, agua para el control, bebida azucarada carbonatada, dosis de 150, 300 y 300 mg/ kg de peso corporal de mentol, glutamato monosódico y tramadol, respectivamente. Las ratas fueron sacrificadas 24 horas después del último día del tratamiento de treinta días. Se realizaron ensayos bioquímicos sobre el plasma y el homogeneizado testicular. Resultados: Hubo aumento significativo (p<0,05) en testosterona, FSH, LH, HDL, TG, fosfolípidos, glucógeno, reducción de la concentración de glutatión, recuento total de espermatozoides, % de cambio de peso testicular, y también hubo una disminución significativa en el porcentaje de defectos en la cola y en el porcentaje de espermatozoides no móviles en los grupos de tratamiento en comparación con el control. Por el contrario, hubo un aumento significativo (p<0,05) en las actividades testiculares de ACP y Na-K ATPasas, pero los niveles de MDA disminuyeron significativamente en todos los grupos de tratamiento. La energía de unión del complejo ouabaína-α-ATPasa es comparable a la de los complejos α-ATPasa con tramadol, glucosa, mentol y glutamato monosódico, respectivamente. Conclusión: La mejora de la calidad del esperma y la función testicular muestran que estos compuestos no fueron perjudiciales para las funciones reproductivas de las ratas Wistar. El análisis de acoplamiento corroboró los efectos de la modulación de la actividad de la ATPasa sobre la motilidad de los espermatozoides.

Introdução: Alguns compostos como os opioides, comumente utilizados, podem afetar o sistema biológico, além de apresentarem alto potencial de dependência. Objetivo: Este estudo avaliou os efeitos de substâncias comumente mal utilizadas na qualidade do esperma e na função testicular em ratos Wistar. Material e métodos: Vinte e cinco ratos Wistar com peso médio de 120 ± 0,1 g foram distribuídos aleatoriamente em cinco grupos de tratamento e receberam por via oral água para controle, bebida gaseificada açucarada, doses de 150, 300 e 300 mg/kg de peso corporal de mentol, glutamato monossódico e tramadol, respectivamente. Os ratos foram sacrificados 24 horas após o último dia do tratamento de trinta dias. Ensaios bioquímicos foram realizados em plasma e homogeneizado testicular. Resultados: Houve aumento significativo (p<0,05) de testosterona, FSH, LH, HDL, TG, fosfolipídios, glicogênio, redução na concentração de glutationa, contagem total de espermatozoides, % de alteração no peso testicular, e também houve diminuição significativa de a porcentagem de defeitos na cauda e a porcentagem de espermatozoides imóveis nos grupos de tratamento em comparação com o controle. Em contraste, houve um aumento significativo (p<0,05) nas atividades testiculares de ACP e Na-K ATPase, mas os níveis de MDA diminuíram significativamente em todos os grupos de tratamento. A energia de ligação do complexo ouabaína-α-ATPase é comparável à dos complexos α-ATPase com tramadol, glicose, mentol e glutamato monossódico, respectivamente. Conclusão: A melhoria da qualidade espermática e da função testicular mostram que estes compostos não foram prejudiciais às funções reprodutivas de ratos Wistar. A análise de docking corroborou os efeitos da modulação da atividade da ATPase na motilidade espermática.

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APA

Nwonuma, C. O., Adedoyin, A. O., Onyemaka, M., Irokanulo, E., Alejolowo, O. O., Udofia, I. A., Ojo, O. A., Adah, D. A., Okeniyi, F. A. y Osemwegie, O. O. (2024). An in vivo and in silico predictive study on the toxicological and modulatory effects of abused substances on sperm quality and testicular function in Wistar rats. Revista Colombiana de Ciencias Químico-Farmacéuticas, 53(2), 513–551. https://doi.org/10.15446/rcciquifa.v53n2.114454

ACM

[1]
Nwonuma, C.O., Adedoyin, A.O., Onyemaka, M., Irokanulo, E., Alejolowo, O.O., Udofia, I.A., Ojo, O.A., Adah, D.A., Okeniyi, F.A. y Osemwegie, O.O. 2024. An in vivo and in silico predictive study on the toxicological and modulatory effects of abused substances on sperm quality and testicular function in Wistar rats. Revista Colombiana de Ciencias Químico-Farmacéuticas. 53, 2 (jun. 2024), 513–551. DOI:https://doi.org/10.15446/rcciquifa.v53n2.114454.

ACS

(1)
Nwonuma, C. O.; Adedoyin, A. O.; Onyemaka, M.; Irokanulo, E.; Alejolowo, O. O.; Udofia, I. A.; Ojo, O. A.; Adah, D. A.; Okeniyi, F. A.; Osemwegie, O. O. An in vivo and in silico predictive study on the toxicological and modulatory effects of abused substances on sperm quality and testicular function in Wistar rats. Rev. Colomb. Cienc. Quím. Farm. 2024, 53, 513-551.

ABNT

NWONUMA, C. O.; ADEDOYIN, A. O.; ONYEMAKA, M.; IROKANULO, E.; ALEJOLOWO, O. O.; UDOFIA, I. A.; OJO, O. A.; ADAH, D. A.; OKENIYI, F. A.; OSEMWEGIE, O. O. An in vivo and in silico predictive study on the toxicological and modulatory effects of abused substances on sperm quality and testicular function in Wistar rats. Revista Colombiana de Ciencias Químico-Farmacéuticas, [S. l.], v. 53, n. 2, p. 513–551, 2024. DOI: 10.15446/rcciquifa.v53n2.114454. Disponível em: https://revistas.unal.edu.co/index.php/rccquifa/article/view/114454. Acesso em: 17 jul. 2024.

Chicago

Nwonuma, Charles Obiora, Adeola Oluwaseun Adedoyin, Melody Onyemaka, Emenike Irokanulo, Omokolade Oluwaseyi Alejolowo, Inemesit Asukwo Udofia, Oluwafemi Adeleke Ojo, Deborah A. Adah, Funmilayo Abimbola Okeniyi, y Omorefosa O. Osemwegie. 2024. «An in vivo and in silico predictive study on the toxicological and modulatory effects of abused substances on sperm quality and testicular function in Wistar rats». Revista Colombiana De Ciencias Químico-Farmacéuticas 53 (2):513-51. https://doi.org/10.15446/rcciquifa.v53n2.114454.

Harvard

Nwonuma, C. O., Adedoyin, A. O., Onyemaka, M., Irokanulo, E., Alejolowo, O. O., Udofia, I. A., Ojo, O. A., Adah, D. A., Okeniyi, F. A. y Osemwegie, O. O. (2024) «An in vivo and in silico predictive study on the toxicological and modulatory effects of abused substances on sperm quality and testicular function in Wistar rats», Revista Colombiana de Ciencias Químico-Farmacéuticas, 53(2), pp. 513–551. doi: 10.15446/rcciquifa.v53n2.114454.

IEEE

[1]
C. O. Nwonuma, «An in vivo and in silico predictive study on the toxicological and modulatory effects of abused substances on sperm quality and testicular function in Wistar rats», Rev. Colomb. Cienc. Quím. Farm., vol. 53, n.º 2, pp. 513–551, jun. 2024.

MLA

Nwonuma, C. O., A. O. Adedoyin, M. Onyemaka, E. Irokanulo, O. O. Alejolowo, I. A. Udofia, O. A. Ojo, D. A. Adah, F. A. Okeniyi, y O. O. Osemwegie. «An in vivo and in silico predictive study on the toxicological and modulatory effects of abused substances on sperm quality and testicular function in Wistar rats». Revista Colombiana de Ciencias Químico-Farmacéuticas, vol. 53, n.º 2, junio de 2024, pp. 513-51, doi:10.15446/rcciquifa.v53n2.114454.

Turabian

Nwonuma, Charles Obiora, Adeola Oluwaseun Adedoyin, Melody Onyemaka, Emenike Irokanulo, Omokolade Oluwaseyi Alejolowo, Inemesit Asukwo Udofia, Oluwafemi Adeleke Ojo, Deborah A. Adah, Funmilayo Abimbola Okeniyi, y Omorefosa O. Osemwegie. «An in vivo and in silico predictive study on the toxicological and modulatory effects of abused substances on sperm quality and testicular function in Wistar rats». Revista Colombiana de Ciencias Químico-Farmacéuticas 53, no. 2 (junio 6, 2024): 513–551. Accedido julio 17, 2024. https://revistas.unal.edu.co/index.php/rccquifa/article/view/114454.

Vancouver

1.
Nwonuma CO, Adedoyin AO, Onyemaka M, Irokanulo E, Alejolowo OO, Udofia IA, Ojo OA, Adah DA, Okeniyi FA, Osemwegie OO. An in vivo and in silico predictive study on the toxicological and modulatory effects of abused substances on sperm quality and testicular function in Wistar rats. Rev. Colomb. Cienc. Quím. Farm. [Internet]. 6 de junio de 2024 [citado 17 de julio de 2024];53(2):513-51. Disponible en: https://revistas.unal.edu.co/index.php/rccquifa/article/view/114454

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