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Analysis of phytochemical composition, antioxidant activity, and β‐glucuronidase inhibition potential of Arisaema tortuosum leaf extract
Análisis de la composición fitoquímica, actividad antioxidante y potencial de inhibición de la β-glucuronidasa del extracto de hoja de Arisaema tortuosum
Análise da composição fitoquímica, atividade antioxidante e potencial de inibição da β-glucuronidase do extrato da folha de Arisaema tortuosum
DOI:
https://doi.org/10.15446/rcciquifa.v52n2.106817Palavras-chave:
Arisaema tortuosum, antioxidant activity, anti-inflammatory potential , total flavonoid content, total polyphenolic content (en)Arisaema tortuosum, actividad antioxidante , potencial antiinflamatorio, contenido de polifenoles totales (es)
Arisaema tortuosum , atividade antioxidante , potencial anti-inflamatório , teor total de flavonoides , teor total de polifenóis (pt)
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Introduction: Medicinal plants contain essential phytochemicals and are used in traditional herbal therapy for ages. These phytochemicals are bioactive compounds and possess significant antibacterial and antifungal properties in addition to more biologically important potentials. The important bioactive phytochemicals include polyphenolic compounds and flavonoids. Arisaema tortuosum has been reported as a medicinal plant in literature and is used in treating many health issues. Aim: To investigate the phytochemical composition, total polyphenolic content (TPC), total flavonoid content (TFC), antioxidant capacity, and β-glucuronidase inhibition potential of the extract obtained from leaves of A. tortuosum. Methods: The phytochemical composition, TPC, TFC, and antioxidant capacity of the extract was analysed. β-glucuronidase inhibition assay was used to determine β-glucuronidase inhibition potential. The dose-dependent data was used to determine IC50 value of the extract. Results: The gas chromatography-mass spectroscopy (GC-MS) analysis identified the fifty-three components from the leaf extract that was observed to possess a significant volume of TPC and TFC. 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay revealed a IC50 value of 936 μg/mL indicating a high antioxidant activity of the extract. Conclusion: The study revealed the presence of many biologically important phytochemicals and a rich number of total polyphenols and flavonoids in leaf extract of A. tortuosum signifying the potential biomedical applications.
Introducción: las plantas medicinales contienen compuestos fitoquímicos esenciales y se utilizan en la terapia herbolaria tradicional desde hace siglos. Estos fitoquímicos son compuestos bioactivos y poseen importantes propiedades antibacterianas y antifúngicas, además de potenciales efectos biológicamente más importantes. Los fitoquímicos bioactivos importantes incluyen compuestos polifenólicos y flavonoides. En la literatura se ha reportado a la Arisaema tortuosum como una planta medicinal y que se usa para tratar muchos problemas de salud. Objetivo: investigar la composición fitoquímica, el contenido polifenólico total (TPC), el contenido total de flavonoides (TFC), la capacidad antioxidante y el potencial de inhibición de la β-glucuronidasa del extracto obtenido de las hojas de A. tortuosum. Métodos: se analizó la composición fitoquímica, TPC, TFC y la capacidad antioxidante del extracto. Se utilizó el ensayo de inhibición de β-glucuronidasa para determinar el potencial de inhibición de este agente. Los datos dependientes de la dosis se usaron para determinar el valor IC50 del extracto. Resultados: el análisis de cromatografía de gases-espectroscopía de masas (GC-MS) permitió identificar cincuenta y tres componentes del extracto de hojas, que se observó que poseían un volumen significativo de TPC y TFC. El ensayo de 1,1-difenil-2-picril-hidrazilo (DPPH) reveló un valor IC50 de 936 μg/mL, lo que indica una alta actividad antioxidante del extracto. Conclusión: este estudio reveló la presencia de muchos compuestos fitoquímicos biológicamente importantes y una gran cantidad de polifenoles totales y flavonoides en el extracto de hoja de A. tortuosum, lo que significa aplicaciones biomédicas potenciales.
Introdução: as plantas medicinais contêm compostos fitoquímicos essenciais e têm sido utilizadas na terapia tradicional à base de plantas há séculos. Esses fitoquímicos são compostos bioativos e possuem importantes propriedades antibacterianas e antifúngicas, além de efeitos potencialmente mais importantes biologicamente. Fitoquímicos bioativos importantes incluem compostos polifenólicos e flavonóides. Arisaema tortuosum tem sido relatada na literatura como planta medicinal e utilizada para o tratamento de diversos problemas de saúde. Objetivo: investigar a composição fitoquímica, teor de polifenóis totais (TPC), teor de flavonoides totais (TFC), capacidade antioxidante e potencial de inibição da β-glucuronidase do extrato obtido das folhas de A. tortuosum. Métodos: foram analisados a composição fitoquímica, TPC, TFC e a capacidade antioxidante do extrato. O ensaio de inibição da β-glucuronidase foi utilizado para determinar o potencial de inibição deste agente. Os dados dependentes da dose foram usados para determinar o valor IC50 do extrato. Resultados: a análise por cromatografia gasosa-espectroscopia de massa (GC-MS) permitiu a identificação de cinquenta e três componentes do extrato da folha, que apresentaram um volume significativo de TPC e TFC. O ensaio de 1,1-difenil-2-picril-hidrazil (DPPH) revelou um valor de IC50 de 936 μg/ mL, indicando uma alta atividade antioxidante do extrato. Conclusão: este estudo revelou a presença de muitos compostos fitoquímicos biologicamente importantes e uma grande quantidade de polifenóis totais e flavonóides no extrato da folha de A. tortuosum, significando potenciais aplicações biomédicas.
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